Progress and challenges in applying CRISPR/Cas techniques to the genome editing of trees

Pak, Solme; Li, Chenghao

doi:10.48130/fr-2022-0006

Cited by 7 publications

(2 citation statements)

References 99 publications

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“…However, this strategy seems not efficient for lincRNA, as the transcriptional level of PRIR1 could be suppressed in none of all 5 independent transgenic lines of PRIR1-RNAi (Supporting Information: Figure 5). Further CRISPR/Cas techniques should be applied to knock out PRIR1 (Pak & Li, 2022). In any case, the PRIR1 overexpression results suggested that PRIR1 may function in promoting bud break in hybrid aspen.…”

Section: Identification Of New Regulators Mediating Seasonal Growthmentioning

confidence: 99%

Multilayer regulatory landscape and new regulators identification for bud dormancy release and bud break in Populus

Hu,

Wu,

Zhu

et al. 2024

Plant Cell & Environment

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For trees originating from boreal and temperate regions, the dormancy‐to‐active transition, also known as bud dormancy release and bud break, are crucial processes that allow trees to reactive growth in the spring. The molecular mechanisms underlying these two processes remain poorly understood. Here, through integrative multiomics analysis of the transcriptome, DNA methylome, and proteome, we gained insights into the reprogrammed cellular processes associated with bud dormancy release and bud break. Our findings revealed multilayer regulatory landscapes governing bud dormancy release and bud break regulation, providing a valuable reference framework for future functional studies. Based on the multiomics analysis, we have determined a novel long intergenic noncoding RNA named Phenology Responsive Intergenic lncRNA 1 (PRIR1) plays a role in the activation of bud break. that the molecular mechanism of PRIR1 has been preliminary explored, and it may partially promote bud break by activating its neighbouring gene, EXORDIUM LIKE 5 (PtEXL5), which has also been genetically confirmed as an activator for bud break. This study has revealed a lncRNA‐mediated regulatory mechanism for the control of bud break in Populus, operating independently of known regulatory pathways.

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Section: Identification Of New Regulators Mediating Seasonal Growthmentioning

confidence: 99%

Multilayer regulatory landscape and new regulators identification for bud dormancy release and bud break in Populus

Hu,

Wu,

Zhu

et al. 2024

Plant Cell & Environment

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“…Method optimization [127] The polyethylene glycol (PEG)-mediated delivery method has also been employed in fruit tree genome-editing systems (Table 1) since it is especially useful for these species where the production of transgenic plants is very slow [128]. This CRISPR/Cas delivery method has been proposed as a good strategy to produce transgene-free edited plants by delivering ribonucleoprotein [127].…”

Section: Ph5mentioning

confidence: 99%

CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding

Martín-Valmaseda,

Devin,

Ortuño-Hernández

et al. 2023

IJMS

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CRISPR (short for “Clustered Regularly Interspaced Short Palindromic Repeats”) is a technology that research scientists use to selectively modify the DNA of living organisms. CRISPR was adapted for use in the laboratory from the naturally occurring genome-editing systems found in bacteria. In this work, we reviewed the methods used to introduce CRISPR/Cas-mediated genome editing into fruit species, as well as the impacts of the application of this technology to activate and knock out target genes in different fruit tree species, including on tree development, yield, fruit quality, and tolerance to biotic and abiotic stresses. The application of this gene-editing technology could allow the development of new generations of fruit crops with improved traits by targeting different genetic segments or even could facilitate the introduction of traits into elite cultivars without changing other traits. However, currently, the scarcity of efficient regeneration and transformation protocols in some species, the fact that many of those procedures are genotype-dependent, and the convenience of segregating the transgenic parts of the CRISPR system represent the main handicaps limiting the potential of genetic editing techniques for fruit trees. Finally, the latest news on the legislation and regulations about the use of plants modified using CRISPR/Cas systems has been also discussed.

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Conventional Versus Non-Conventional Methods of Propagation of Forest Tree Species: Applications and Limitations

Manohar,

Shukla,

Shahina

et al. 2024

Biotechnological Approaches for Sustaining Forest Trees and Their Products

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Progress and challenges in applying CRISPR/Cas techniques to the genome editing of trees

Cited by 7 publications

References 99 publications

Multilayer regulatory landscape and new regulators identification for bud dormancy release and bud break in Populus

Multilayer regulatory landscape and new regulators identification for bud dormancy release and bud break in Populus

CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding

Conventional Versus Non-Conventional Methods of Propagation of Forest Tree Species: Applications and Limitations

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