This study presents an experimental rig of a multi-evaporator refrigeration system, in which the pressure difference between two evaporators can be maintained by using both the pressure-regulating valve (PRV) and electronic expansion valve (EEV)-based ejector. The proposed EEV-based ejector that is used to partially recover the throttling losses of the PRV consists of an EEV and the main body of an ejector. The established experimental system can work in both PRV-based mode and ejector-based mode by switching the valves. Via experimental means, the performances of both modes were evaluated by varying the cooling loads. Moreover, the effects of the spindle-blocking area percentage of the EEV-based ejector and the condensing temperature on the system performance were identified. The results showed that: (1) the system performance of the ejector-based mode was 3.6% higher than the PRV-based mode; (2) both entrainment ratio and coefficient of performance dropped along with the increase in ejector spindle-blocking area percentage; (3) compared to ejector spindle-blocking area percentage, the condensing temperature had a more evident influence on the system performance.