Progress and controversies in unraveling the glomerular filtration mechanism

Kuppe, Christoph; Moeller, Marcus J.

doi:10.1097/mnh.0000000000000116

Cited by 9 publications

(7 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The electrokinetic model is an expansion of the pore model, which considers also electrical effects [20,29,[34][35][36][37][38]. According to this model, glomerular filtration works in two steps, which occur simultaneously (Figure 5B).…”

Section: Mathematical Modelling Of Vegf Back-filtrationmentioning

confidence: 99%

Inverse correlation between vascular endothelial growth factor back-filtration and capillary filtration pressures

Kuppe

Rohlfs

Grepl

et al. 2018

Nephrology Dialysis Transplantation

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Mathematical Modelling Of Vegf Back-filtrationmentioning

confidence: 99%

Inverse correlation between vascular endothelial growth factor back-filtration and capillary filtration pressures

Kuppe

Rohlfs

Grepl

et al. 2018

Nephrology Dialysis Transplantation

Self Cite

View full text Add to dashboard Cite

show abstract

“…On average about 180 liters of plasma holding 13 ± 3 kilograms of protein is filtered by the kidneys daily . The ratio of urine protein concentration to plasma protein concentration is also known as sieving coefficient.…”

Section: Human Urine Composition and Anatomical Inputsmentioning

confidence: 99%

“…A comparison of high quality proteomics data of disparate patient origins found about 26% of total urine protein identifications were also found in plasma after removing proteins known to be prostatic secretions, and only about 15% of total plasma proteins were also found in urine [24]. On average about 180 liters of plasma holding 13 ± 3 kilograms of protein is filtered by the kidneys daily [25]. The ratio of urine protein concentration to plasma protein concentration is also known as sieving coefficient.…”

Section: Human Urine Composition and Anatomical Inputsmentioning

confidence: 99%

Biomarker discovery in mass spectrometry‐based urinary proteomics

Thomas

Hao

Ricke

et al. 2016

Proteomics Clinical Apps

119

View full text Add to dashboard Cite

Urinary proteomics has become one of the most attractive topics in disease biomarker discovery. Mass spectrometry (MS)-based proteomic analysis has advanced continuously and emerged as a prominent tool in the field of clinical bioanalysis. However, only few protein biomarkers have made their way to validation and clinical practice. Biomarker discovery is challenged by many clinical and analytical factors including, but not limited to, the complexity of urine and the wide dynamic range of endogenous proteins in the sample. This article highlights promising technologies and strategies in the MS-based biomarker discovery process, including study design, sample preparation, protein quantification, instrumental platforms, and bioinformatics. Different proteomics approaches are discussed, and progresses in maximizing urinary proteome coverage and standardization are emphasized in this review. MS-based urinary proteomics has great potential in the development of noninvasive diagnostic assays in the future, which will require collaborative efforts between analytical scientists, systems biologists, and clinicians.

show abstract

“…Nevertheless, an extensive understanding of, for example, key kidney functions, including mechanisms of glomerular filtration and the formation of urine from primary urine to the final voided urine, remains to be achieved (Saritas et al 2015; Maas et al 2016). The combined use of proteomics, lipidomics, metabolomics and comprehensive genomic analyses, together with advanced systems biology algorithms, offers the promise of major new discoveries for the benefit of patients with kidney disease.…”

Section: Introductionmentioning

confidence: 99%

Urinary extracellular vesicles. A promising shortcut to novel biomarker discoveries

Barreiro

Holthöfer

2017

Cell Tissue Res

View full text Add to dashboard Cite

Proteomic and genomic techniques have reached full maturity and are providing unforeseen details for the comprehensive understanding of disease pathologies at a fraction of previous costs. However, for kidney diseases, many gaps in such information remain to inhibit major advances in the prevention, treatment and diagnostics of these devastating diseases, which have enormous global impact. The discovery of ubiquitous extracellular vesicles (EV) in all bodily fluids is rapidly increasing the fundamental knowledge of disease mechanisms and the ways in which cells communicate with distant locations in processes of cancer spread, immunological regulation, barrier functions and general modulation of cellular activity. In this review, we describe some of the most prominent research streams and findings utilizing urinary extracellular vesicles as highly versatile and dynamic tools with their extraordinary protein and small regulatory RNA species. While being a highly promising approach, the relatively young field of EV research suffers from a lack of adherence to strict standardization and carefully scrutinized methods for obtaining fully reproducible results. With the appropriate guidelines and standardization achieved, urine is foreseen as forming a unique, robust and easy route for determining accurate and personalized disease signatures and as providing highly useful early biomarkers of the disease pathology of the kidney and beyond.

show abstract

Progress and controversies in unraveling the glomerular filtration mechanism

Abstract: Understanding how the glomerular filter functions is a prerequisite to investigate the pathogenesis of proteinuric glomerular diseases and the link between glomerular proteinuria and cardiovascular disease.

Cited by 9 publications

References 34 publications

Inverse correlation between vascular endothelial growth factor back-filtration and capillary filtration pressures

Inverse correlation between vascular endothelial growth factor back-filtration and capillary filtration pressures

Biomarker discovery in mass spectrometry‐based urinary proteomics

Urinary extracellular vesicles. A promising shortcut to novel biomarker discoveries

Contact Info

Product

Resources

About