Progress and promise of alternative animal and non-animal methods in biomedical research

Freires, Irlan Almeida; Colón, David F.; Soares, Lélio Fernando Ferreira; Costa, Isabela Silva; Araújo, Leonardo Pereira de; Breseghello, Isadora; Abdalla, Henrique Ballassini; Lazarini, Josy Goldoni; Rosalen, Pedro Luiz; Pigossi, Suzane Cristina; Franchin, Marcelo

doi:10.1007/s00204-023-03532-1

Cited by 10 publications

(5 citation statements)

References 117 publications

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“…When setting up the experiment, the physiological characteristics of the animal's body were taken into account [8]. After slaughter, a pathological examination of the rat corpses was carried out [9]. Biomaterial sampling was carried out in accordance with the methodological recommendations for sampling [10].…”

Section: Materials and Methods Of Researchmentioning

confidence: 99%

“…При постановке опыта учитывали физиологические особенности организма животных [8]. После забоя проводили патологоанатомический осмотр трупов крыс [9]. Отбор проб биоматериала проводили в соответствии с методическими рекомендациями по отбору проб [10].…”

Section: материал и методы исследованияunclassified

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Характеристика Бактериальной Микрофлоры У Крыс При Массированном Инфицировании Брюшной Полости

MUSSAYEVA,

ABDRESHOV,

YEGOROVA

et al. 2023

Микробиология Және Вирусология

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Isolation of pathogens of infectious diseases isolated from animals, their identification based on the study of biological properties, determination of antibiotic resistance is an urgent task. During of inflammation of the abdominal cavity on a biological system in vivo, cultures of microorganisms were isolated on laboratory rats killed for diagnostic purposes, and their sensitivity to antibiotics was studied. As a result of the studies, it was found that on the 2nd and 5th days after infection of rats with coprological exudate an inflammatory process developed in the abdominal cavity, characterized by a disorder of blood and lymph circulation in blood and lymphatic vessels, in capillaries and venules, edema, hemorrhages in internal organs and vessels, necrosis tissues (on the 5th day after infection). As a result of bacteriological examination of exudate from the abdominal cavity, exudate from the abdominal cavity and liver of experimental rats, pathogenic and conditionally pathogenic microorganisms were isolated. Cultures were identified based on the study of cultural-morphological, tinctorial and biochemical properties. Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa were isolated from samples of biological material of rats. The sensitivity of isolated microorganisms to antibiotics was studied. Knowledge of the etiology of infectious inflammation of the abdominal cavity of rats, the effect on pathogens of antibiotics of various groups, the correct selection of the drug contribute to the elimination of infection in the shortest possible time. Выделение возбудителей инфекционных заболеваний от животных, их идентификация на основе изучения биологических свойств, определение антибиотикорезистентности является актуальной задачей. В результате проведенных исследований установлено, что на 2-е и 5-е сутки после инфицирования крыс копрологическим экссудатом в брюшную полость развивался воспалительный процесс, характеризующийся расстройством крово- и лимфообращения в кровеносных и лимфатических сосудах, в капиллярах и венулах, отеком, геморрагиями во внутренних органах и сосудах, некрозом тканей (на 5 сутки после заражения). В результате бактериологического исследования экссудата из брюшной полости и печени опытных крыс были выделены патогенные и условно патогенные микрорганизмы. Культуры идентифицировали на основе изучения культурально-морфологических, тинкториальных и биохимических свойств. Из проб биологического материала крыс выделены Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. Изучена чувствительность выделенных микроорганизмов к антибиотикам. Знание этиологии инфекционного воспаления брюшной полости крыс, действие на возбудителей антибиотиков различных групп, правильный подбор препарата способствуют ликвидации инфекции в кратчайшие сроки.

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Section: Materials and Methods Of Researchmentioning

confidence: 99%

Section: материал и методы исследованияunclassified

Характеристика Бактериальной Микрофлоры У Крыс При Массированном Инфицировании Брюшной Полости

MUSSAYEVA,

ABDRESHOV,

YEGOROVA

et al. 2023

Микробиология Және Вирусология

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“…Lastly, it is important to point out that the use of animals models has raised several ethical questions such as the animal welfare, the possible alternatives, the moral considerations and regulations to name only a few [128][129][130]. However, efforts have been made to propose alternative solutions including computational and in silico methods [131,132]. Despite these differences and challenges, researchers are continuing to develop new and improved methods for determining nanomaterial toxicity, and we can expect to learn more about the safety of graphene in the upcoming years.…”

Section: Our Current Understanding Of the Safety Of Carbon Nanotubes ...mentioning

confidence: 99%

Carbon Nanotubes and Graphene Materials as Xenobiotics in Living Systems: Is There a Consensus on Their Safety?

Gendron,

Bubak

2023

JoX

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Carbon nanotubes and graphene are two types of nanomaterials that have unique properties and potential applications in various fields, including biomedicine, energy storage, and gas sensing. However, there is still a debate about the safety of these materials, and there is yet to be a complete consensus on their potential risks to human health and the environment. While some studies have provided recommendations for occupational exposure limits, more research is needed to fully understand the potential risks of these materials to human health and the environment. In this review, we will try to summarize the advantages and disadvantages of using carbon nanotubes and graphene as well as composites containing them in the context of their biocompatibility and toxicity to living systems. In addition, we overview current policy guidelines and technical regulations regarding the safety of carbon-based nanomaterials.

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“…The A. salina toxicity test has several advantages over the mouse assay, including ease of use, low cost, quick findings, and the ability to examine a large number of samples, in addition to ethical benefits ( Freires et al, 2016 ). It was first presented by Meyer and colleagues ( Meyer et al, 1982 ), and since then it has been extensively used in toxicology ( Kerster and Schaeffer, 1983 ; Sanchez-Fortun and Barahona, 2009 ; Hamidi et al, 2014 ; Freires et al, 2023 ). However, its application in snake venom research is limited ( Okumu et al, 2020 , 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Artemia salina toxicity assay as a substitute of the mouse lethality assay in the determination of venom-induced toxicity and preclinical efficacy of antivenom

Araya,

Okumu,

Durán

et al. 2024

Toxicon: X

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Progress and promise of alternative animal and non-animal methods in biomedical research

Cited by 10 publications

References 117 publications

Характеристика Бактериальной Микрофлоры У Крыс При Массированном Инфицировании Брюшной Полости

Характеристика Бактериальной Микрофлоры У Крыс При Массированном Инфицировании Брюшной Полости

Carbon Nanotubes and Graphene Materials as Xenobiotics in Living Systems: Is There a Consensus on Their Safety?

Assessment of the Artemia salina toxicity assay as a substitute of the mouse lethality assay in the determination of venom-induced toxicity and preclinical efficacy of antivenom

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