2003

DOI: 10.1038/sj.gt.3301983

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Progress and prospects: naked DNA gene transfer and therapy

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Cited by 380 publications

(255 citation statements)

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“…Figure 7 insets show the corresponding fluorescent images (derived from EGFP expression) after 24 and 48 h incubation, which further verify the gene delivery performance. Considering the average size of LAu NVs (103.8 nm), the transfection of pDNA was probably due to receptor‐mediated macropinocytosis or transient membrane pores through the cytoplasm of cells in accordance with previous reports 39, 40. Even though the transfection efficiencies of the NVs were comparable with lipofectamine owing to the protection against direct exposure of pDNA to the intracellular trafficking enzymes, the efficiencies were still smaller than those from cationic polymer‐ or lipid‐based gene carriers41, 42 because of no polycationic addition compounds (+2.9 mV, zeta potential).…”

Section: Resultssupporting

confidence: 91%

Photoinduced Rapid Transformation from Au Nanoagglomerates to Drug‐Conjugated Au Nanovesicles

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2017

Advanced Science

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Gold (Au) agglomerates (AGs) are reassembled using Triton X‐100 (T) and doxorubicin (D) dissolved in ethanol under 185 nm photoirradiation to form TAuD nanovesicles (NVs) under ambient gas flow conditions. The positively charged Au particles are then electrostatically conjugated with the anionic chains of TD components via a flowing drop (FD) reaction. Photoirradiation of the droplets in a tubular reactor continues the photophysicochemical reactions, resulting in the reassembly of Au AGs and TD into TAuD NVs. The fabricated NVs are electrostatically collected onto a polished aluminum rod in a single‐pass configuration. The dispersion of NVs is employed for bioassays to confirm uptake by cells and accumulation in tumors. The chemo‐photothermal activity is determined both in vitro and in vivo. Different combinations of components are also used to fabricate NVs using the FD reaction, and these NVs are suitable for gene delivery as well. This newly designed gaseous single‐pass process results in the reassembly of Au AGs for incorporation with TD without the need of batch wet chemical reactions, modifications, separations, or purifications. Thus, this process offers an efficient platform for preparing biofunctional Au nanostructures that requires neither complex physicochemical steps nor special storage techniques.

“…Figure 7 insets show the corresponding fluorescent images (derived from EGFP expression) after 24 and 48 h incubation, which further verify the gene delivery performance. Considering the average size of LAu NVs (103.8 nm), the transfection of pDNA was probably due to receptor‐mediated macropinocytosis or transient membrane pores through the cytoplasm of cells in accordance with previous reports 39, 40. Even though the transfection efficiencies of the NVs were comparable with lipofectamine owing to the protection against direct exposure of pDNA to the intracellular trafficking enzymes, the efficiencies were still smaller than those from cationic polymer‐ or lipid‐based gene carriers41, 42 because of no polycationic addition compounds (+2.9 mV, zeta potential).…”

Section: Resultssupporting

confidence: 91%

Photoinduced Rapid Transformation from Au Nanoagglomerates to Drug‐Conjugated Au Nanovesicles

¹

,

²

,

³

2017

Advanced Science

View full text Add to dashboard Cite

Gold (Au) agglomerates (AGs) are reassembled using Triton X‐100 (T) and doxorubicin (D) dissolved in ethanol under 185 nm photoirradiation to form TAuD nanovesicles (NVs) under ambient gas flow conditions. The positively charged Au particles are then electrostatically conjugated with the anionic chains of TD components via a flowing drop (FD) reaction. Photoirradiation of the droplets in a tubular reactor continues the photophysicochemical reactions, resulting in the reassembly of Au AGs and TD into TAuD NVs. The fabricated NVs are electrostatically collected onto a polished aluminum rod in a single‐pass configuration. The dispersion of NVs is employed for bioassays to confirm uptake by cells and accumulation in tumors. The chemo‐photothermal activity is determined both in vitro and in vivo. Different combinations of components are also used to fabricate NVs using the FD reaction, and these NVs are suitable for gene delivery as well. This newly designed gaseous single‐pass process results in the reassembly of Au AGs for incorporation with TD without the need of batch wet chemical reactions, modifications, separations, or purifications. Thus, this process offers an efficient platform for preparing biofunctional Au nanostructures that requires neither complex physicochemical steps nor special storage techniques.

“…The primary challenge in applying gene delivery to these applications is inefficient delivery, with extracellular and intracellular barriers both limiting the efficiency. In non-viral approaches, plasmid is complexed with cationic lipids or polymers to facilitate transfection in vitro and in vivo [1][2][3]. Complexation can enhance interactions between positively charged DNA complexes and the negatively charged cellular membrane, in addition to providing stability against degradation [4] and facilitating intracellular trafficking.…”

Section: Introductionmentioning

confidence: 99%

Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

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2008

Acta Biomaterialia

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Substrate-mediated gene delivery describes the immobilization of gene therapy vectors to a biomaterial, which enhances gene transfer by exposing adhered cells to elevated DNA concentrations within the local microenvironment. Surface chemistry has been shown to affect transfection by nonspecifically immobilized complexes using self-assembled monolayers (SAMs) of alkanethiols on gold. In this report, SAMs were again used to provide a controlled surface to investigate whether the presence of oligo(ethylene glycol) (EG) groups in a SAM could affect complex morphology and enhance transfection. EG groups were included at percentages that did not affect cell adhesion. Nonspecific complex immobilization to SAMs containing combinations of EG-and carboxylic acidterminated alkanethiols resulted in substantially greater transfection than surfaces containing no EG groups or SAMs composed of EG groups combined with other functional groups. Enhancement in transfection levels could not be attributed to complex binding densities or release profiles. Atomic force microscopy imaging of immobilized complexes revealed that EG groups within SAMs affected complex size and appearance and could indicate the ability of these surfaces to preserve complex morphology upon binding. The ability to control the morphology of the immobilized complexes and influence transfection levels through surface chemistry could be translated to scaffolds for gene delivery in tissue engineering and diagnostic applications.

“…Although plasmid DNA provides transfection in vivo, complexing DNA with non-viral vectors, cationic lipids or polymers, can facilitate internalization and transfection in vitro and in vivo [1][2][3]. Complexation can facilitate uptake by enhancing interactions between positively charged DNA complexes and the negatively charged cellular membrane, in addition to providing stability against degradation [4].…”

Section: Introductionmentioning

confidence: 99%

Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning

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2005

Acta Biomaterialia

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Gene transfer has many potential applications in basic and applied sciences. In vitro, DNA delivery can be enhanced by increasing the concentration of DNA in the cellular microenvironment through immobilization of DNA to a substrate that supports cell adhesion. Substrate-mediated delivery describes the immobilization of DNA, complexed with cationic lipids or polymers, to a biomaterial or substrate. As surface properties are critical to the efficiency of the surface delivery approach, selfassembled monolayers (SAMs) of alkanethiols on gold were used to correlate surface chemistry of the substrate to binding, release, and transfection of non-specifically immobilized complexes. Surface hydrophobicity and ionization were found to mediate both DNA complex immobilization and transfection, but had no effect on complex release. Additionally, SAMs were used in conjunction with soft lithographic techniques to imprint substrates with specific patterns, resulting in patterned DNA complex deposition and transfection, with transfection efficiencies in the patterns nearing 40%. Controlling the interactions between complexes and substrates, with the potential for patterned delivery, can be used to locally enhance or regulate gene transfer, with applications to tissue engineering scaffolds and transfected cell arrays.

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