Progress in affinophoresis

Affinity Capillary Electrophoresis: A Sensitive Tool for the Study of Molecular Interactions and Its Use in Microscale Analyses

“…In this format, the for gene expression and specific sequences of poly-nucleotides (35). We proposed a mobile affinity ligand At present, a practical problem in temperature control is that most commercially available CE instrusystem, ''affinophoresis,'' in which affinity ligands were attached to ionic polymers to form an ''affinophore'' that ments are designed so that the ends of the capillary project from the temperature-controlled housing for acspecifically accelerates the electrophoresis of the molecules having affinity for the ligand (4,36,37). Though cess to the electrode vessels.…”

“…One can choose either of the interacting components to be electropho-the following equation is derived (29)(30)(31)(32)37). resed as a zone, depending on availability and the ease of observing the mobility change.…”

“…We have introduced the use of charged soluthe absence of B, i.e., the mobility of the free protein (m A ), and its detection time increases. Note that the ble polymers, such as succinylpolylysine, as mobile matrices for affinity electrophoresis (4,36,37). We call the concentration of the free ligand in the protein zone has become identical to that in the solution originally used charged matrix bearing ligands the ''affinophore'' and the electrophoresis in its presence ''affinophoresis.''…”

Prevention of evaporation of small-volume sample solutions for capillary electrophoresis using a mineral-oil overlay

“…Among several modes of affinity capillary electrophoresis, affinophoresis is especially useful. The coupling of an affinity ligand to a multiply charged matrix forms an affinophore that can result in a large change in the migration velocity of proteins, when bound to the ligand, i.e., affinophoresis [4,5] (Fig. 1).…”

Capillary affinophoresis as a versatile tool for the study of biomolecular interactions: a mini-review

“…Affinophoresis is an electrophoretic method that combines bioaffinity and electrophoresis; that is, electrophoresis of a biomolecule such as a protein is carried out under the influence of a mobile affinity ligand, which is called an affinophore (Shimura and Kasai, 1982, 1984, 1987a,b, 1996aShimura, 1990). Although the term affinophore can cover almost all soluble molecules that have both electric charges and an affinity ligand, it is preferable to use this term to refer to an artificial molecule designed for a special purpose, such as an affinity adsorbent in the case of affinity chromatography.…”