Progress in biopulping of non-woody materials: Chemical, enzymatic and ultrastructural aspects of wheat straw delignification with ligninolytic fungi from the genus Pleurotus

Martı́nez, Ángel T.

doi:10.1016/0168-6445(94)90084-1

Cited by 113 publications

(58 citation statements)

References 16 publications

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“…Continued expression of POH2 and POH3 by emerging aerial hyphae would coat them with the observed hydrophobic rodlet layer. As has been surmised for SC3 of S. commune (Wosten et al, 1994b) and ABH3 of A. bisporus (Lugones et al, 1998), secreted POH2 and POH3 could also play a role in facilitating lignin degradation, an important activity of P. ostreatus (Martinez et al, 1994).…”

Section: Discussionmentioning

confidence: 98%

ldentif ication of three differentially expressed hydrophobins in Pleurotus ostreatus (oyster mushroom)

1998

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Three proteins with characteristic features of class I hydrophobins, designated POH1, POH2 and POH3, were isolated from the basidiomycete PIeurotus ostreatus. Based on N-terminal sequence analyses, their cDNAs were isolated using RT-PCR; the cDNAs and corresponding genes were sequenced and their regulation studied. POHI is expressed in the fruiting bodies but not in vegetative mycelium. The regulation of fWf2 and poH3 is tightly correlated. Both genes are switched off in the fruiting bodies but abundantly expressed in the vegetative mycelium of both monokaryon and dikaryon. POH2 and POH3 were isolated from the culture medium and from aerial hyphae. Co-purified POH2 and POH3 assembled in witro into a protein membrane with a typical rodlet pattern as found previously with other hydrophobins. Similar structures were detected on the surface of aerial hyphae.

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Section: Discussionmentioning

confidence: 98%

ldentif ication of three differentially expressed hydrophobins in Pleurotus ostreatus (oyster mushroom)

1998

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“…5 A). Analytical IEF showed that the isoenzymes have slightly different PI, 3.75 for MP-1 and 3.65 for MP-2 (Fig. 5B), which may explain their separation on Mono-Q.…”

Section: Properties Of the Mp Isoenzymesmentioning

confidence: 99%

“…Some species, including Pleurotus eryngii, have the capacity to remove lignin preferentially (i.e., with limited degradation of cellulose) [ 11, a characteristic relevant to biotechnological delignification processes for feed production 121 and paper-pulp manufacture [3].…”

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confidence: 99%

Purification and Catalytic Properties of Two Manganese Peroxidase Isoenzymes from Pleurotus eryngii

Martı́nez

Ruíz-Dueñas

Guillén

et al. 1996

European Journal of Biochemistry

343

244

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The ligninolytic basidiomycetes Pleurotus eryngii, Pleurotus ostreatus, Pleurotus pulmonarius and Pleurotus sajor-caju did not exhibit detectable levels of manganese peroxidase (MP) when grown in liquid media with ammonium tartrate as N source. However, after examination of cells grown on different organic N-based media, high M P activity was obtained in peptone medium, up to nearly 3 U/ml in cultures of I? eryngii. Moreover, Mnz+ supplementation was not used to produce MP, since all Mn2+ concentrations assayed (1 -4000 pM) inhibited production of this enzyme in liquid medium.Two MP isoenzymes were purified to homogeneity from shaken or stationary cultures of I? eryngii grown in peptone medium. The purification process (which included chromatography on Biorad Q-cartridge, Sephacryl S-200 and Mono-Q) attained 56% activity yield with a purification factor of 25. The isoenzymes differed in PI (3.75 and 3.65), N-terminal sequence and some catalytic properties. They were in some aspects (e.g., molecular mass of 43 kDa) similar to Phanerochaete chrysosporium MP but exhibited some distinct characteristics, including Mn"-independent peroxidase activities against 2,6-dimethoxyphenol and veratryl alcohol, and higher resistance to H,O,. Recent studies have shown that M P are ubiquitous enzymes in ligninolytic fungi, but the results obtained suggest that differences in catalytic properties probably exist between different Mn2 ' -oxidizing peroxidases produced by these fungi.

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“…P. ostreatus is widely reported to be a selective degrader of lignin [30,53]. A systematic screening of ligninolytic fungi [54] reported that lignin was preferentially degraded from wheat straw by P. ostreatus. White rot fungi, of which P. ostreatus is an example, possess unique ligninolytic enzymes that completely degrade lignin to CO 2 and H 2 O [55].…”

Section: Ligninmentioning

confidence: 99%

Co-Substrating of Peanut Shells with Cornstalks Enhances Biodegradation by Pleurotus ostreatus

FN¹,

Yusuf²

2016

J Bioremed Biodeg

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IntroductionPeanut shells (PS) are abundant agro-industrial waste products that are recalcitrant to degradation under natural conditions [1]. The increasing expansion of peanut production has led to accumulation of large quantities of these shells all over the world. In the US alone, peanut production increased by 45% between 2011 and 2012, bringing production to 3.04 million metric tons [2]. Globally, 45.6 million metric tons of peanuts are produced annually [3]. It has been estimated that for every kg of peanuts produced, 230-300 g of peanut shells are generated [4]. Therefore, as much as 13.7 million metric tons of peanut shells are churned out every year, most of which are dumped into the environment or burned [5,6]. Peanut shells are a renewable resource that could be targeted for purposeful use in the food, feed, paper and bioenergy industries. However, as yet, few if any value-added uses exist for them [7].Over the years, various attempts to utilize peanut shells have been made. Small fractions were incorporated into animal feed, especially for cattle [8], while some authors have experimented on their use as dietary fiber for humans [9]. Other non-food research trials include uses in pulp production [10], feedstock for bioethanol production [11,12], particle board component [13], mulch and activated charcoal [7]. However, the major drawback to their use in large scale industrial operations is the high lignin content.Much like other agricultural lignocellulolysic biomass, peanut shells are composed mainly of lignin, cellulose and hemicellulose. Lignin, a complex polymer, is the most recalcitrant component of plant biomass [14] and binds tightly to, and provides a physical seal around, cellulose and hemicellulose [11,[15][16][17][18], thereby protecting them against attack by hydrolytic enzymes. The restricted bioavailability of these sugars and other components is thought to be largely physical, with lignin molecules reducing the surface area available to enzymatic penetration and activity [19]. In order to gain access to the sugars in cellulose and AbstractWorld consumption of peanuts has increased tremendously, resulting in abundance of peanut shell waste. The high lignin content of peanut shells limits their bioconversion to useful products or recycling. Therefore, the synergy in co-substrating peanut shells (PS) and cornstalks (CS) to enhance biodegradation was evaluated. Various compositions of peanut shells and cornstalks (% dry weight) herein called co-substrate -90PS:10CS, 75PS:25CS, 50PS:50CS, 25PS:75CS, 10PS:90CS, and two controls Cont1-100PS and Cont2-100CS were studied under solid state fermentation (SSF) with a white rot fungus, Pleurotus ostreatus for 120 days. A two-factorial experiment in a completely randomized design (CRD) was used. Results showed that substrate composition and fermentation time were important variables in substrate degradation. Lignin degradation, losses in organic matter, cellulose and hemicellulose increased with time. Increasing the composition of CS in co-substrates...

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Progress in biopulping of non-woody materials: Chemical, enzymatic and ultrastructural aspects of wheat straw delignification with ligninolytic fungi from the genus Pleurotus

Cited by 113 publications

References 16 publications

ldentif ication of three differentially expressed hydrophobins in Pleurotus ostreatus (oyster mushroom)

ldentif ication of three differentially expressed hydrophobins in Pleurotus ostreatus (oyster mushroom)

Purification and Catalytic Properties of Two Manganese Peroxidase Isoenzymes from Pleurotus eryngii

Co-Substrating of Peanut Shells with Cornstalks Enhances Biodegradation by Pleurotus ostreatus

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