Progress in capillary electrophoresis of biomarkers and metabolites between 2002 and 2005

Huck, Christian W.; Bakry, Rania; Bonn, Günther K.

doi:10.1002/elps.200500493

Cited by 29 publications

(24 citation statements)

References 151 publications

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“…For some general reviews regarding this technique in the analysis of proteins that include glycoproteins, see refs [13][14][15][16]. For specific reviews of CE of glycoproteins, see refs [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

CZE of human alpha‐1‐acid glycoprotein for qualitative and quantitative comparison of samples from different pathological conditions.

et al. 2006

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Alpha‐1‐acid glycoprotein (AGP) presents different forms, which may arise from differences in the amino acid sequence and/or in the glycosidic part of the protein. Changes in forms of AGP have been described in literature as a possible tumor marker. While most previous works have approached the study of glycopeptides and/or glycans obtained after fragmentation of the protein, in this work, a CZE method is developed to separate up to eleven peaks of intact forms of AGP. A computer program developed in our laboratory is used to select the migration parameters that make possible an accurate assignment of AGP peaks. Electropherograms of AGP samples purified from sera of cancer patients and healthy donors are qualitatively and quantitatively compared. Percentages of correct assignment of AGP peaks close to 100% are achieved by using either the migration time of each peak relative to that of the EOF marker or the effective electrophoretic mobility of the peaks. The computer program permits to select, among different hypotheses for peak allotment, that one providing the highest accuracy of assignment. In this way, some peaks with different charge‐to‐mass ratio and a different distribution of area percentage of AGP forms are observed when comparing samples from sick and healthy individuals. Thus, a method that permits to compare AGP forms existing in sera of individuals with different pathophysiological situations has been developed. A potential for using AGP forms analyzed by CZE as a disease marker and for using this technique for screening purposes is envisaged.

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Section: Introductionmentioning

confidence: 99%

CZE of human alpha‐1‐acid glycoprotein for qualitative and quantitative comparison of samples from different pathological conditions.

et al. 2006

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“…From the detection of microparticles, organic compounds, and polymers to the separation of organic/inorganic ions, CE has become a very practical tool for chemical analysis and environmental pollutions investigation [1][2][3][4]. In medical research and applications, this technology has been used extensively in detecting, monitoring, and controlling diseases via biomarkers and metabolites of cells [5]. Interestingly, microbiology and genetics have benefited much from the application of CE to the identification and characterization of cells.CE has wide ranging applications from analyses of protein, peptides, DNA fragments, and sequencing to detection of single molecular mutation [6].…”

Section: Introductionmentioning

confidence: 99%

Application of CE for determination of DNA base composition

Hua

Naganuma

2007

Electrophoresis

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DNA base composition expressed as mol% of guanine plus cytosine (% GC) or GC content is a key parameter of bacterial taxonomy and genomic analyses. Direct chemical determination methods such as HPLC as well as indirect methods based on physical properties of deoxyribonucleic acid (DNA), melting point (T(m)), and buoyant density (B(d)) have been conventionally applied to determine the GC content. However, these methods require relatively large amounts of sample DNA, time, and labor. We have developed a protocol to determine the GC content by fine separation of nucleosides with CZE. Genomic DNAs with known GC content from 23 bacterial strains were determined by CE at the optimized conditions of 27 degrees C, 20 kV in 50 mM of NaHCO(3) (pH 9.0) and 70 mM SDS added. Nucleosides from <1 microg of DNA hydrolyzed with nuclease-P1 and bacterial alkaline phosphatase were separated in a 75 microm wide and 80 cm long silica capillary. The nucleoside peak areas were determined at 254 nm in less than 12 min. The CE-based determination of GC content requires only small amounts of DNA, and thus should be applicable to environmental genomics (metagenomics), as >90% of environmental micro-organisms are nonculturable and produce only small amounts of genomic DNA.

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“…viruses) [10][11][12][13][14]. With its advantageous propertieshigh efficiency, rapidity, and low consumption of samples and reagents -CE has become a popular tool for protein profile analysis [15][16][17][18]. Recently, we have developed CE methods for protein analysis under discontinuous conditions [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Combining capillary electrophoresis with laser‐induced fluorescence detection for the analysis of Escherichia coli lysates

Shu-feng

Chiu

2009

Electrophoresis

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With the goal of improving water quality control, in this study we combined CE-LIF for the analysis of Escherichia coli lysates under discontinuous conditions. Prior to injecting a large-volume protein sample, a plug of 0.2% w/v SDS was injected into the capillary filled with 2.0 M Tris-borate (TB) solution (pH 10.3). During the courses of the process stacking, proteins migrated against the electroosmotic flow (EOF) and entered a 1.7% w/v poly(ethylene oxide) solution that has been prepared in 200 mM TB (pH 9.0). The stacked proteins were then separated through sieving and then detected at the cathodic end. The use of TB solutions containing 0.01% w/v poly(ethylene oxide) was essential for the preparation of the protein samples and E. coli lysates to minimize the extent of protein adsorption on the capillary wall. Under the optimized CE-LIF conditions, we detected beta-Galactosidase with and without concentration at levels as low as 0.23 and 7.52 nM, respectively. Our approach allowed the detection of 3 x 10(2) E. coli cells based on the characteristic peaks of its lysates; in addition, we could detect the presence of 20 E. coli cells in a 50 mL sample of pond water within 24 h. In terms of the analyte migration time, the relative standard deviation of this CE-LIF method was less than 1.3%. We also extended the practicality of this technique by applying it to the separation of mixtures of E. coli, Enterobacter aerogenes, and Salmonella enterica.

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Progress in capillary electrophoresis of biomarkers and metabolites between 2002 and 2005

Cited by 29 publications

References 151 publications

CZE of human alpha‐1‐acid glycoprotein for qualitative and quantitative comparison of samples from different pathological conditions.

CZE of human alpha‐1‐acid glycoprotein for qualitative and quantitative comparison of samples from different pathological conditions.

Application of CE for determination of DNA base composition

Combining capillary electrophoresis with laser‐induced fluorescence detection for the analysis of Escherichia coli lysates

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