Progress in cell culture systems for pathological research

Abstract

Cell culture is a well-established standard technique and a fundamental tool in biology and medicine. Establishment of a novel culture method by meeting various challenges can sometimes open up new fields of cell biology and medicine. An artificial microenvironment for cultured cells is made up of complicated factors, including cytokines, scaffold material type, cell-cell interactions, and physical stress. To replicate the tissue architecture, cell-cell interactions, and specific physical microenvironment, we previously demonstrated the effectiveness of a three-dimensional culture system, and further established two simple culture systems: air-liquid interface (ALI) and fluid flow stress (FFS). A three-dimensional collagen gel culture system can replicate cell-cell interactions in vitro. As skin is constantly exposed to air, the ALI system closely mimicked the skin microenvironment and maintained the homeostasis of the epidermis and dermis. The ALI culture system also revealed the possibility of skin regeneration through ectopic mesenchymal cell involvement. Fluid streaming and shear stress were recently demonstrated to constitute the critical microenvironment for various cell types. The FFS system demonstrated that fluid streaming induced epithelial-mesenchymal transition of mesothelial cells, leading to peritoneal fibrosis. Our novel culture systems will hopefully open up new fields of regenerative medicine and pathological research.

Atividade Antileishmania De Óleos Essenciais Sobre Formas De Leishmania Infantum: Uma Revisão

“…Para esse tipo celular, as garrafas de cultura necessitam possuir uma carga negativa. Esse tipo de cultura consiste na distribuição de uma população de células em concentrações de milhares ou milhões, em placas ou garrafas plásticas, próprias para este fim, juntamente com meios de cultura contendo os nutrientes essenciais para o crescimento e multiplicação das células (AOKI et al, 2016;KOLEDOVA, 2017).…”

In vitro three-dimensional cell cultures for bone sarcomas

“…Scaffold 3D systems consist in a structural support that favours cell adhesion, proliferation, migration, cell-to-cell interaction and signalling [33] , [34] . Natural scaffolds are based on molecules that are present in the ECM such as collagen, gelatine and derivatives [35] , [36] , complex matrix (e.g., commercial Matrigel™) and hydrogels [37] , [38] , and polysaccharides as alginate, chitosan or hyaluronic acid [39] , [40] , [41] , [42] , [43] . Whereas the main advantage of these natural scaffolds is their biocompatibility, their production and inter-batches variability are the main issues of these materials.…”

Cell Spinpods: A Simple Inexpensive Device to Determine Suspension Culture and Toxicity Effects on Renal Proximal Tubular Cells

“…Generating fluid shear stress in vitro presents a variety of challenges (reviewed in [2]). Several technologies are available including orbital shakers, parallel plates, microfluidics with peristaltic pumps, and perfused hollow fibers [8,13,[21][22][23][24]. But most of these options require expensive equipment and are rarely practical for high throughput analysis due to high cost and/or large vessel volumes [2].…”