Progress in chemical luminescence-based biosensors: A critical review

Roda, Aldo; Mirasoli, Mara; Michelini, Elisa; Fusco, Massimo Di; Zangheri, Martina; Cevenini, Luca; Roda, Barbara; Simoni, Patrizia

doi:10.1016/j.bios.2015.06.017

Cited by 195 publications

(131 citation statements)

References 172 publications

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“…Here, a photochemical emission takes place during the binding of the biomarker to the recognition element. The advantages of chemiluminescence-based biosensors lie in the fact that they require very simple measurement equipment (Roda et al, 2015). Qu et al (2013) reported an immuno-sensor for CEA using chemiluminescence immunoassay along with immunomagnetic separation.…”

Section: Chemiluminescence Based Detection Techniquesmentioning

confidence: 99%

Recent advances in biosensor development for the detection of cancer biomarkers

Jayanthi

Das

Saxena

2017

Biosensors and Bioelectronics

421

169

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Section: Chemiluminescence Based Detection Techniquesmentioning

confidence: 99%

Recent advances in biosensor development for the detection of cancer biomarkers

Jayanthi

Das

Saxena

2017

Biosensors and Bioelectronics

421

169

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“…Biosensors represent a promising tool to supplement sophisticated techniques, due to their unique characteristics, such as high selectivity, relative low cost, facility of automation and the possibility of data acquisition in real time [13].…”

Section: Biosensorsmentioning

confidence: 99%

Applications and Perspectives of Biosensors for Diagnostics in Infectious Diseases

Etchegaray¹

2018

CJMI

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Infectious diseases are considered a major cause of morbidity and/or mortality worldwide, despite the development of preventive and control strategies. Infectious diseases result from the invasion of body tissues by disease-causing microorganisms. The occurrence and development of infectious diseases are closely associated with the functional state of the immune system. Interleukins play significant roles in modulating the immune response of the host during the course of an infectious disease, contributing to the maintenance of the homeostasis of the immune system. The use of biosensors for the detection of interleukins is a new and important strategy for the diagnosis of the infection and the immunological response profile that occurs for specific diseases, thus helping in the effective diagnosis, and allowing physicians to design treatment in a faster way, which contributes to better prognosis. For example, in the oral environment there are two major infectious diseases: caries and periodontitis. The microbes involved are respectively, Streptococcus mutans and Porphyromonasgingivalis. The presence of specific interleukins in saliva or crevicular fluid will indicate that there is potentially an infectious disease, which could be connected with either caries or periodontal disease. For instance, an indicative for caries could be the presence of IL-6 in saliva, while periodontal disease would be confirmed by the presence of IL-17 in crevicular fluid. In addition, both diseases could be confirmed by the detection of higher levels of each of one of the pathogens. Considering the development of biosensor design and construction, the answer to all these question could be obtained in a single analysis.

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“…[1][2][3][4][5][6][7][8][9][10][11] In order to increase the automation of luminescent analytical procedures, the detection of ECL response by flow injection analysis system (FIA) is an interesting option. [12,13] Flow analysis is characterized by highly reproducible and throughput sampling.…”

Section: A Miniaturized Flow Injection Analysis System For Electrogenmentioning

confidence: 99%

A Miniaturized Flow Injection Analysis System for Electrogenerated Chemiluminescence−Based Assays

et al. 2017

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A new miniaturized flow injection analysis (FIA) system for developing electrochemiluminescence (ECL)-based assays is presented. Novel thin-layer flowÀcell (TLFCL) screen-printed carbon electrodes integrated in one-channel flow cells were used as the electrode surface. These TLFCL electrodes, combined with miniaturized electrochemical and optical instrumentation, allowed a high degree of automation and simplicity of the assay. The performance of this high-throughput flow system was corroborated with a proof-of-concept centered on H 2 O 2 detection. For that purpose, an ECL transduction chemistry mechanism based on a electrochemiluminescence resonance energy transfer (ECL-RET) donorÀacceptor strategy, between an electro-oxidized luminophore (luminol) to a fluorophore (fluorescein), in the presence of the analyte of interest, was carried out. The results showed that fluorescein ECL-RET luminescent response increases the sensitivity of H 2 O 2 detection by up to one order of magnitude, in comparison with a luminolÀH 2 O 2 fluorescein-free assay. All experiments were performed in a decentralized, small-sized, easy-to-use FIA system coupled to ECL detection instrumentation.The light emitted from electrogenerated chemiluminescence (ECL) is a process in which electrochemically generated species combine to undergo electron-transfer reactions to form excited, light-emitting species, giving place to the development of high sensitive and selective assays. [1][2][3][4][5][6][7][8][9][10][11] In order to increase the automation of luminescent analytical procedures, the detection of ECL response by flow injection analysis system (FIA) is an interesting option. [12,13] Flow analysis is characterized by highly reproducible and throughput sampling. In addition, sequential injection analysis allows a considerable reduction in the consumption of the sample and reagents. [14] Bearing in mind that ECL based advances are moving towards the development of portable, high-throughput and point-of-care testing systems, [8,15] disposable screen-printed electrodes coupled to a small-sized, non-conventional, FIA system appears as an attractive possibility.For that purpose, in this work, screen-printed electrodes integrated in one channel flow-cell (TLFCL, Thin layer flow-cell) were used as the electrode surface. In these electrodes a transparent slide, that usefully allows the detection of air bubbles inside the cell, is attached over the screen-printed electrodes platform delimiting a flow channel. The injection of sample volume is done through an "in-line luer" injection port, placed closest to the electrochemical cell, where the injection can be easily controlled by operator through a precise syringe. This configuration brings important advantages since it simplifies operability and effectiveness of working in FIA systems. A similar format was already reported in two previous works [16,17] where enzymatic amperometric based sensors were developed. However, this is the first time that these electrodes, integrated in the necessary accessori...

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Progress in chemical luminescence-based biosensors: A critical review

Cited by 195 publications

References 172 publications

Recent advances in biosensor development for the detection of cancer biomarkers

Recent advances in biosensor development for the detection of cancer biomarkers

Applications and Perspectives of Biosensors for Diagnostics in Infectious Diseases

A Miniaturized Flow Injection Analysis System for Electrogenerated Chemiluminescence−Based Assays

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