Progress in DNA Aptamers as Recognition Components for Protein Functional Regulation

Hu, Lingling; Liu, Ke; Ren, Guolan; Liang, Jiangong; Wu, Yuan

doi:10.1007/s40242-022-2124-8

Cited by 5 publications

(3 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The unique properties of aptamers enable their great potential for exploring allosteric modulators of protein functions, such as ligand-induced conformational changes. [28,29] In 1998, Bates et al identified the target of the G-rich oligonucleotide AS1411 as the NCL protein. [30] AS1411 exhibited antitumor effects in a variety of cancer types, including breast cancer, kidney cancer, and lung cancer.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A Non‐G‐Quadruplex DNA Aptamer Targeting NCL for Diagnosis and Therapy in Bladder Cancer

Liu

Pei

et al. 2023

Adv Healthcare Materials

View full text Add to dashboard Cite

Bladder cancer (BC) is a highly aggressive malignant tumor affecting the urinary system, characterized by metastasis and a poor prognosis that often leads to limited therapeutic success. This study aims to develop a novel DNA aptamer for the diagnosis and treatment of BC using a tissue‐based systematic evolution of ligands by an exponential enrichment (SELEX) process. By using SELEX, this work successfully generates a new aptamer named TB‐5, which demonstrates a remarkable and specific affinity for nucleolin (NCL) in BC tissues and displays marked biocompatibility both in vitro and in vivo. Additionally, this work shows that NCL is a reliable tissue‐specific biomarker in BC. Moreover, according to circular dichroism spectroscopy, TB‐5 forms a non‐G‐quadruplex structure, distinguishing it from the current NCL–targeting aptamer AS1411, and exhibits a distinct binding region on NCL compared to AS1411. Notably, this study further reveals that TB‐5 activates NCL function by promoting autophagy and suppressing the migration and invasion of BC cells, which occurs by disrupting mRNA transcription processes. These findings highlight the critical role of NCL in the pathological examination of BC and warrant more comprehensive investigations on anti‐NCL aptamers in BC imaging and treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The unique properties of aptamers enable their great potential for exploring allosteric modulators of protein functions, such as ligand‐induced conformational changes. [ 28,29 ] In 1998, Bates et al. identified the target of the G‐rich oligonucleotide AS1411 as the NCL protein.…”

Section: Discussionmentioning

confidence: 99%

A Non‐G‐Quadruplex DNA Aptamer Targeting NCL for Diagnosis and Therapy in Bladder Cancer

Liu

Pei

et al. 2023

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…Aptamers interact with proteins via hydrogen bonding, electrostatic interactions, hydrophobic interactions and van der Waals forces. − Changing the structure of aptamers can tune their binding affinity toward target proteins. − By way of illustration, the introduction of hydrophobic amino-acid-like side chains into aptamers can form additional hydrophobic contacts, enhancing the interactions of aptamers with thrombin dramatically and achieving highly sensitive detection of thrombin. − Notably, regulating the interactions between aptamers and disease-related proteins allows modulation of protein-mediated cell behaviors, assisting disease diagnosis, treatment and pathological assessment. − For example, aptamers are modified with hydrophobic small-molecule anticancer drugs to improve their interactions with target proteins, facilitating selective cellular uptake and thereby inhibiting tumor growth . The regulation of aptamer–protein interactions thus serves as a universal platform not only for realizing the highly sensitive and selective detection of biomolecules, but also for boosting the discovery of disease biomarkers and potential drug targets.…”

Section: Introductionmentioning

confidence: 99%

Aptamer–Protein Interactions: From Regulation to Biomolecular Detection

Ji,

Wei,

Zhang

et al. 2023

Chem. Rev.

View full text Add to dashboard Cite

Serving as the basis of cell life, interactions between nucleic acids and proteins play essential roles in fundamental cellular processes. Aptamers are unique single-stranded oligonucleotides generated by in vitro evolution methods, possessing the ability to interact with proteins specifically. Altering the structure of aptamers will largely modulate their interactions with proteins and further affect related cellular behaviors. Recently, with the in-depth research of aptamer–protein interactions, the analytical assays based on their interactions have been widely developed and become a powerful tool for biomolecular detection. There are some insightful reviews on aptamers applied in protein detection, while few systematic discussions are from the perspective of regulating aptamer–protein interactions. Herein, we comprehensively introduce the methods for regulating aptamer–protein interactions and elaborate on the detection techniques for analyzing aptamer–protein interactions. Additionally, this review provides a broad summary of analytical assays based on the regulation of aptamer–protein interactions for detecting biomolecules. Finally, we present our perspectives regarding the opportunities and challenges of analytical assays for biological analysis, aiming to provide guidance for disease mechanism research and drug discovery.

show abstract

Computational and experimental investigations of a novel aptamer targeting oxidized low-density lipoprotein

Khongwichit,

Nualla-ong,

Prompat

et al. 2024

Computers in Biology and Medicine

View full text Add to dashboard Cite

Progress in DNA Aptamers as Recognition Components for Protein Functional Regulation

Cited by 5 publications

References 87 publications

A Non‐G‐Quadruplex DNA Aptamer Targeting NCL for Diagnosis and Therapy in Bladder Cancer

A Non‐G‐Quadruplex DNA Aptamer Targeting NCL for Diagnosis and Therapy in Bladder Cancer

Aptamer–Protein Interactions: From Regulation to Biomolecular Detection

Computational and experimental investigations of a novel aptamer targeting oxidized low-density lipoprotein

Contact Info

Product

Resources

About