Progress in enzyme immobilization in ordered mesoporous materials and related applications

Zhou, Zhou; Hartmann, Martin

doi:10.1039/c3cs60059a

Cited by 528 publications

(378 citation statements)

References 193 publications

(158 reference statements)

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“…This behaviour occurred because immobilization by covalent binding becomes stronger binding of enzymes on the silica aerogel to prevent leaching because of the chemical bonding between the amino groups of the lipase and the terminal aldehyde of the support is irreversible and resist. 32,33 It is worth mentioning that some reports demonstrate that covalent binding of the enzyme with a suitable bifunctional agent improves the stability of the immobilized enzymes. In the immobilization of lipases from porcine pancreas by the method of covalent binding in the presence of PVA as additive in silica aerogel, there was a half-life of 6 hours at 10 cycles of reuse with residual activity of 70% in the final reuse in the emulsification reaction of olive oil.…”

Section: The Total Activity Recovery Yield (H %) Results For the Immentioning

confidence: 99%

The Novel Mesoporous Silica Aerogel Modified With Protic Ionic Liquid for Lipase Immobilization

Barbosa¹,

Lisboa²,

Silva³

et al. 2016

Química Nova

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Recebido em 24/08/2015; aceito em 22/12/2015; publicado na web em 11/02/2016Mesoporous silica supports (aerogels) were used to immobilize Burkholderia cepacia lipase (BC) by encapsulation (EN or ENIL), physical adsorption (ADS or ADSIL) and covalent binding (CB or CBIL) into or onto the aerogel modified with protic ionic liquid (PIL). Yield immobilization (Ya) and operational stability were determined by the hydrolytic reaction of olive oil. Ya (37% to 83% by physical adsorption) and operational stability (2 to 23 batches by encapsulation) increased when the support was modified with PIL. For immobilized derivates observed by the BET method, in this case ADS and CB for ADSIL and CBIL, increased pores size was observed, possibly due to the higher amount of BC immobilized conferring Ya and operational stability. This effect was probably attributed to the entry of the enzyme into the pores of the silica aerogel structure. SEM images showed a change in the structure and properties of immobilized lipase derived with PIL. A characteristic FTIR band was obtained for the silanol groups and amides I, IV and V, demonstrating the efficiency of immobilization of BC. The most efficient biocatalysts were ADSIL with regard to yield immobilization and ENIL for operational stability.

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Section: The Total Activity Recovery Yield (H %) Results For the Immentioning

confidence: 99%

The Novel Mesoporous Silica Aerogel Modified With Protic Ionic Liquid for Lipase Immobilization

Barbosa¹,

Lisboa²,

Silva³

et al. 2016

Química Nova

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“…Many studies have examined silica-based organic hybrid mesoporous materials with organosilane, capping molecules/nanoparticles or polymer functional groups for the adsorption and delivery of drugs, genes and enzymes under a range of internal/external stimuli, such as temperature, redox, pH, light, magnetism and ultrasound. 45 However, the studies using PMOs as carriers of drug molecules are still limited.…”

Section: Pmo Materials As Hosts For Drug and Biomoleculesmentioning

confidence: 99%

Periodic mesoporous organosilicas for advanced applications

2014

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In 1999, three groups independently developed a novel class of organic-inorganic nanocomposites known as periodic mesoporous organosilicas (PMOs). The organic functional groups in the frameworks of these solids allow tuning of the surface properties and modification of the bulk properties of the material. This paper provides a comprehensive overview of PMOs and discusses their different functionalities, morphology and applications, such as catalysis, drug delivery, sensing, optics, electronic devices, environmental applications (gas sensing and gas adsorption), biomolecule adsorption and chromatography for which PMOs have been used over the past 5 years.

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“…They are also characterized by high stability, well-developed surface area, and the possibility of surface functionalization with many modifying compounds [16,17]. Due to the unique properties of such materials, especially highly ordered mesoporous structures, they are widely used in the enzyme immobilization [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Amano Lipase A onto Stöber silica surface: process characterization and kinetic studies

Zdarta

Sałek

Kołodziejczak-Radzimska

et al. 2014

Open Chemistry

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The immobilization of Amano Lipase A fromAspergillus niger by adsorption onto Stöber silica matrix obtained by sol-gel method was studied. The effectiveness of the enzyme immobilization and thus the usefulness of the method was demonstrated by a number of physicochemical analysis techniques including Fourier Transform Infrared Spectroscopy (FT-IR), elemental analysis (EA), thermogravimetric analysis (TG), porous structure of the support and the products after immobilization from the enzyme solution with various concentration at different times. The analysis of the process' kinetics allowed the determination of the sorption parameters of the support and optimization of the process. The optimum initial concentration of the enzyme solution was found to be 5 mg mL -1 , while the optimum time of the immobilization was 120 minutes. These values of the variable parameters of the process were obtained by as ensuring the immobilization of the largest possible amount of the biocatalyst at the most economically beneficial aspects of the process.

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Progress in enzyme immobilization in ordered mesoporous materials and related applications

Cited by 528 publications

References 193 publications

The Novel Mesoporous Silica Aerogel Modified With Protic Ionic Liquid for Lipase Immobilization

The Novel Mesoporous Silica Aerogel Modified With Protic Ionic Liquid for Lipase Immobilization

Periodic mesoporous organosilicas for advanced applications

Immobilization of Amano Lipase A onto Stöber silica surface: process characterization and kinetic studies

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