Progress in precise and predictable genome editing in plants with base editing

Abstract

Base editors are gene editing tools that allow targeted nucleic acid conversions, most commonly C>T and A>G, through pairing of deamination domains with impaired nucleases. Multiple deaminase domains and architectures have been demonstrated in planta across a wide array of species, with both cytosine and adenine base editing frequencies being observed at over 80%. The ability of base editors to introduce nucleic acid diversity while maintaining the same reading frame should make them powerful tools for plant genetic editing moving forward.

Targeted Mutagenesis of the Multicopy Myrosinase Gene Family in Allotetraploid Brassica juncea Reduces Pungency in Fresh Leaves across Environments

“…Therefore, reducing the aforementioned ‘mustard bomb’ reaction and producing leafy greens with a reduced pungency in flavor and/or odor would enable a broader consumption of fresh, highly nutritious leafy greens. The advent of precision breeding technologies [ 14 ] such as CRISPR nucleases [ 15 ], base editors [ 16 , 17 ], and prime editing [ 18 , 19 ] has enabled opportunities for the rapid development of diverse and nutritious foods.…”