Progress in Studies on the Etiology and Serologic Diagnosis of Enzootic Bovine Leukosis

Mussgay, M.; Kaaden, Oskar‐Rüger

doi:10.1007/978-3-642-66853-1_2

Cited by 19 publications

(7 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding conflicts with reports that infected cattle possess antibodies to most BLV proteins (Wuu et al, 1977;Mussgay & Kaaden, 1978;Deshayes et al, 1980;Ferrer, 1980). It is not known whether there are alterations in expression of BLV genes in infected or transformed cells or if cattle with lymphoma have a response different to that of clinically normal infected cattle.…”

Section: Introductioncontrasting

confidence: 86%

See 1 more Smart Citation

Alterations in Humoral Immune Response to Bovine Leukaemia Virus Antigens in Cattle with Lymphoma

Heeney

Valli

Montesanti

1988

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYSera collected from cattle with enzootic bovine lymphoma (EBL) were compared to sera from clinically normal bovine leukaemia virus (BLV)-infected cattle for immunoglobulin concentration and for antibodies detecting BLV proteins tested using three different viral isolates. Monoclonal antibodies to bovine immunoglobulin isotypes were used in Western blot analysis to identify isotype reactivity to specific viral antigens. IgG titres to BLV were determined by ELISA. Serum immunoglobulin (G1, G2 and M) concentrations were assessed by radial immunodiffusion. Although EBL was associated with reduced total immunoglobulin production, sera from cattle with EBL had significantly (P< 0-001) higher specific IgG titres and produced antibodies against a greater and more varied number of BLV proteins than did sera from clinically normal BLV-infected cattle. Variations were consistent within groups of cattle and did not depend on the viral isolate used.

show abstract

Section: Introductioncontrasting

confidence: 86%

“…The humoral immune response is strong (Mussgay & Kaaden, 1978;Burny et al, 1980;Ferrer, 1980) with high blood levels of cytotoxic antibodies to cells expressing BLV proteins (Portetelle et al, 1978). Rising antibody titres appear to be stimulated by virus persistence and replication.…”

Section: Introductionmentioning

confidence: 99%

Alterations in Humoral Immune Response to Bovine Leukaemia Virus Antigens in Cattle with Lymphoma

Heeney

Valli

Montesanti

1988

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…The concept that Enzootic Bovine Leukosis is an infectious disease rests upon 40 years of epidemiological and experimental observations (see 2, 3, 10 for recent reviews). No small contribution to wide acceptance of that concept was the repeated successful transmission of persistent lymphocytosis and/or tumor by inoculation into cattle or sheep of a diversity of biological material from affected cattle.…”

Section: Introductionmentioning

confidence: 99%

Detection by Immunodiffusion- and Radioimmunoassay-Tests of Antibodies to Bovine Leukemia Virus Antigens in Sera of Experimentally Infected Sheep and Cattle

Mammerickx

Portetelle

Burny

et al. 2010

Zentralblatt Für Veterinärmedizin Reihe B

View full text Add to dashboard Cite

Summary Cattle and sheep were experimentally inoculated with blood from animals infected by BLV or affected by sporadic leukosis. Evolution of antibody to bovine leukemia virus antigens was observed over a period of six years. Antibody levels were established by two methods: agar gel immunodiffusion (AGID) and radioimmunoassay (RIA). Bovine leukemia virus (BLV) from bovine origin was able to reinfect cattle, after passage in sheep or goat. The first serological response to inoculation appeared between the 15th and the 30th day. Inoculation by the oral or intradermal route gave the same results. Inoculation at birth of animals with passive immunity could not induce an infection. It thus seems that colostrum from an infected dam is not infectious to offspring. Evolution of antibody levels was progressive until death with tumor of the inoculated animal. Antibody levels did not permit one to prejudge evolution of the tumoral disease. Sporadic leukosis material did not induce a serological response to BLV antigens and did not protect an animal against subsequent inoculation with bovine leukemia virus. After inoculation, onset of antibody and increase of antibody level were sudden; decrease of antibody level in animals with passive immunity was also sharp. RIA allowed more accurate antibody level determination than AGID test. Sensitivity of RIA was higher than AGID. At very low titer RIA gave non‐specific positive reactions. It thus seemed that in practice RIA and AGID tests had the same range of reliable sensitivity. In studies on BLV infection, they are probably among the best diagnostic methods. Zusammenfassung Antikörperentwicklung in Seren von experimentell mit bovinem Leukämie‐Virus (BLV) infizierten Schafen und Rindern mittels Immunodiffusionstest und Radioimmunoassay dargestellt Rinder und Schafe wurden experimentell mit Blut infiziert, welches von Tieren stammte, die mit BLV infiziert waren oder die die sporadische Form der Leukose zeigten. Die Entwicklung der gegen BLV gerichteten Antikörper wurde über einen Zeitraum von 6 Jahren verfolgt. Dazu dienten zwei verschiedene Methoden, die Agargelimmunodiffusion (AGID) und der Radioimmunoassay (RIA). Von Rindern isoliertes BLV war auch nach einer Schafoder Ziegenpassage infektiös für Rinder. Die erste serologische Antwort auf die Infektion trat zwischen dem 15. und 30. Tag auf. Orale oder intradermale Inokulation ergab keine Unterschiede. Inokulation von neugeborenen Tieren mit passiver Immunität konnte keine Infektion hervorrufen. Daraus kann abgeleitet werden, daß Kolostrum eines BLV‐infizierten Muttertieres für neugeborene Tiere nicht infektiös ist. Die Höhe des Antikörpertiters stieg an, bis die Tiere an Tumoren starben. Der Antikörperspiegel erlaubte keine Aussage über die Entstehung einer tumorösen Erkrankung. Material von sporadischen Leukosefällen induzierte keine serologische Reaktion mit BLV‐Antigen und schützte die Tiere nicht vor einer nachfolgenden Infektion mit BLV. Nach der Inokulation traten sehr schnell Antikörper auf und der Anstieg der Antikörper...

show abstract

“…In the second phase infected cattle show a persistent lymphocytosis of the immunoglobulin-bearing lymphocytes. In the third phase between 10 and 30 O / o of the leukaemic cattle (MUSSGAY and KAADEN, 1978;BURNY et al, 1980) may develop tumours of the lymphoid tissue. The eradication programme for EBL in Western Germany is mainly based on experimental and field observations that a BLV-infection can be detected by antibody screenings prior to the appearance of a persistent lymphocytosis.…”

mentioning

confidence: 99%

Transient Viraemia with Bovine Leukaemia Virus in Bulls

Kaaden¹,

Langè²,

Romanowski³

et al. 2010

Zentralblatt Für Veterinärmedizin Reihe B

View full text Add to dashboard Cite

Summary A total of 432 cattle, predominantly of Holstein‐Friesian breed, was tested for bovine leukemia virus (BLV) by the lymphocyte‐kidney cell co‐cultivation technique. 18 animals (4.17%) harboured infectious BLV in the peripheral blood lymphocytes. These animals also showed low titres of BLV antibodies in the ACIF and ELISA tests, ranging from 1 : 5 to 1 : 16, but gave negative results in the immunodiffusion test. In six bulls, kept under quarantine for twelve months, BLV was transiently detected for three months and could be experimentally transmitted to sheep inoculated s. c. or i. m. with 1 ml. of blood. The bulls developed neither a permanent BLV carrier state nor a lymphocytosis within the observation period. Zusammenfassung Transiente Virämie mit dem Rinderleukosevirus in Bullen Mit Hilfe einer Lymphozyten‐Nierenzellen‐Kokultivationsmethode wurden Blutproben von 432 vorwiegend Schwarzbunten Niederungsrindern auf Rinderleukosevirus (BLV) untersucht. Alle Tiere stammten aus Rinderleukose‐unverdächtigen Beständen und hatten keine im Immundiffusions‐Test mit BLV‐Antigenen nachweisbaren Antikörper. Insgesamt wurden 18 Rinder (4,17%) ermittelt, in deren Blutlymphozyten BLV nachgewiesen werden konnte. Bei diesen Tieren wurden jedoch in der antikomplementären Immunofluoreszenz‐und ELISA‐Technik Antikörpertiter zwischen 1 : 5 und 1 : 16 festgestellt. Bei sechs Bullen wurde während einer 12monatigen Quarantänezeit Rinderleukosevirus über einen Zeitraum von 3 Monaten durch Anzüchtung in der Zellkultur und im Tierversuch mit Schafen nachgewiesen. Die Tiere entwik‐kelten im Beobachtungszeitraum keine persistierende Virämie mit BLV oder Lymphozytose. In den Organen der geschlachteten Tiere konnte BLV nicht nachgewiesen werden. Résumé Virémie transitoire avec le virus de la leucose bovine chez des taureaux Des échantillons de sang de 432 bovins en majorité de la race frisonne ont été testés vis‐à‐vis du virus de la leucose bovine (BLV) à l'aide d'une méthode de cocultivation lymphocytes‐cellules rénales. Tous les animaux provenaient d'exploitations non suspectes de leucose et n'avaient présenté aucun anticorps contre des antigènes BLV au test d'immunodiffusion. 18 bovins en tout (4,17%) ont présenté des lymphocytes sanguins BLV. On a mis en évidence chez ces animaux des titres d'anticorps entre 1 : 5 et 1 : 16 dans l'immunofluorescence anticomplémentaire et la technique ELISA. Le virus de la leucose bovine a été relevé durant 3 mois par culture sur cellules et en expérimentation animale avec des moutons chez 6 taureaux dans une quarantaine de 12 mois. Les animaux n'ont pas développé de virémie avec BLV ou de lymphocytose durant le temps d'observation. LBV n'a pas été mis en évidence dans les organes des animaux abattus. Resumen Viremia transitoria con el virus de la leucemia bovina en toros Con ayuda de una técnica de cultivo a base de linfocitos y células renales, se analizaron las muestras sanguíneas de 432 vacunos pertenecientes en su gran mayoría a la raza berrenda en negro de las tierras bajas en cuanto al virus ...

show abstract

Progress in Studies on the Etiology and Serologic Diagnosis of Enzootic Bovine Leukosis

Abstract: Epizootiologic observations and other studies on bovine leukosis performed during the last 50 years provide cumulative evidence that the disease is primar-

Cited by 19 publications

References 48 publications

Alterations in Humoral Immune Response to Bovine Leukaemia Virus Antigens in Cattle with Lymphoma

Alterations in Humoral Immune Response to Bovine Leukaemia Virus Antigens in Cattle with Lymphoma

Detection by Immunodiffusion- and Radioimmunoassay-Tests of Antibodies to Bovine Leukemia Virus Antigens in Sera of Experimentally Infected Sheep and Cattle

Transient Viraemia with Bovine Leukaemia Virus in Bulls

Contact Info

Product

Resources

About