Progress in the understanding of WRKY transcription factors in woody plants

Long, Lianxiang; Gu, Lijiao; Wang, Shijie; Cai, Hongyu; Wu, Jianghao; Wang, Jinmao; Yang, Minsheng

doi:10.1016/j.ijbiomac.2023.124379

Cited by 20 publications

(7 citation statements)

References 73 publications

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“…To validate the reliability of the chosen reference genes, we analyzed the expression patterns of two specific genes, namely AmWRKY6 and AmWRKY33. WRKY proteins, belonging to one of the largest transcription factor families in plants, play critical roles in diverse aspects, such as plant growth, development, and response to biotic and abiotic stresses [62,63]. Figure 6 shows that when the top two stable reference genes were used for normalization, the expression trends were almost similar, but their expression levels were slightly different.…”

Section: Rt-qpcr Is a Widely Used Technology In Molecular Biology For...mentioning

confidence: 99%

Selection of Reference Genes for Gene Expression Analysis in Acacia melanoxylon under Different Conditions

Chen,

Bai,

et al. 2023

Forests

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The research of functional genes in Acacia melanoxylon, a precious and fast-growing timber species with wide adaptability, has been greatly limited due to the absence of reliable and suitable reference genes. To fill this gap, five different algorithms (comparative ΔCt, NormFinder, geNorm, BestKeeper, and RankAggreg) were employed to assess the expression stability of ten candidate genes under nine different experimental sets and their three combined groups. The results showed that PP2a and RPL4 maintained stable expression in all 144 samples and a group of different tissues or organs. PAT10 and TIP41 were the best-performing genes in different clonal varieties, pinnate compound leaves at different growth states, salt, and indole acetic acid sets. PP2a and PAT10 were the top two choices for gibberellin and abiotic stress groups. PP2a and UBI11 exhibited stable expression in drought treatment. UBI3 combined with OTUD6B, RPL4, or PP2a were identified as the optimal reference genes in the heat, ethephon, or exogenous hormone groups, respectively. The reliability of the selected reference genes was further confirmed by evaluating the expression patterns of AmWRKY6 and AmWRKY33 genes. This study provides the first comprehensive evaluation of reference gene stability in A. melanoxylon and promotes future research on the gene expression analysis of the species.

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Section: Rt-qpcr Is a Widely Used Technology In Molecular Biology For...mentioning

confidence: 99%

Selection of Reference Genes for Gene Expression Analysis in Acacia melanoxylon under Different Conditions

Chen,

Bai,

et al. 2023

Forests

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“…OsWRKY53 was induced by abscisic acid (ABA), jasmonate (JA), salt, drought, darkness, and low temperatures but inhibited by gibberellic acid (GA) and salicylic acid (SA) [10]. The WRKY-TFs in various woody plants were widely involved in responses to biotic and abiotic stresses, as well as in plant growth and development [11]. PeWRKY41 belongs to Class III WRKY and interacts with casein kinase.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of a Ramie (Boehmaeria nivea L. Gaud) Group I WRKY Gene, BnWRKY49, Increases Drought Resistance in Arabidopsis thaliana

Bao,

Zou,

et al. 2024

Plants

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Plants face multiple stresses in their natural habitats. WRKY transcription factors (TFs) play an important regulatory role in plant stress signaling, regulating the expression of multiple stress-related genes to improve plant stress resistance. In this study, we analyzed the expression profiles of 25 BnWRKY genes in three stages of ramie growth (the seedling stage, the rapid-growth stage, and the fiber maturity stage) and response to abiotic stress through qRT-PCR. The results indicated that 25 BnWRKY genes play a role in different growth stages of ramie and were induced by salt and drought stress in the root and leaf. We selected BnWRKY49 as a candidate gene for overexpression in Arabidopsis. BnWRKY49 was localized in the nucleus. Overexpression of BnWRKY49 affected root elongation under drought and salt stress at the Arabidopsis seedling stage and exhibited increased tolerance to drought stress. Further research found that BnWRKY49-overexpressing lines showed decreased stomatal size and increased cuticular wax deposition under drought compared with wild type (WT). Antioxidant enzyme activities of SOD, POD, and CAT were higher in the BnWRKY49-overexpressing lines than the WT. These findings suggested that the BnWRKY49 gene played an important role in drought stress tolerance in Arabidopsis and laid the foundation for further research on the functional analysis of the BnWRKYs in ramie.

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“…Transgenic Arabidopsis exhibited heightened sensitivity to ABA during seed germination and under low temperatures and salinity upon overexpression of ClWRKY20 14 . Additionally, WRKY genes, which respond to drought stress, have also been identified in other crops, providing evidence that WRKY transcription factors are crucial in responding and adapting to drought stress 15 – 18 .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and comprehensive analysis of WRKY transcription factor family in safflower during drought stress

Song,

Hou,

Zeng

et al. 2023

Sci Rep

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The WRKY family is an important family of transcription factors in plant development and stress response. Currently, there are few reports on the WRKY gene family in safflower (Carthamus tinctorius L.). In this study, a total of 82 CtWRKY genes were identified from the safflower genome and could be classified into 3 major groups and 5 subgroups based on their structural and phylogenetic characteristics. The results of gene structure, conserved domain and motif analyses indicated that CtWRKYs within the same subfamily maintained a consistent exon/intron organization and composition. Chromosomal localization and gene duplication analysis results showed that CtWRKYs were randomly localized on 12 chromosomes and that fragment duplication and purification selection may have played an important role in the evolution of the WRKY gene family in safflower. Promoter cis-acting element analysis revealed that the CtWRKYs contain many abiotic stress response elements and hormone response elements. Transcriptome data and qRT-PCR analyses revealed that the expression of CtWRKYs showed tissue specificity and a strong response to drought stress. Notably, the expression level of the CtWRKY55 gene rapidly increased more than eightfold under drought treatment and rehydration, indicating that it may be a key gene in response to drought stress. These results provide useful insights for investigating the regulatory function of the CtWRKY gene in safflower growth and development, as well as identifying key genes for future molecular breeding programmes.

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Progress in the understanding of WRKY transcription factors in woody plants

Cited by 20 publications

References 73 publications

Selection of Reference Genes for Gene Expression Analysis in Acacia melanoxylon under Different Conditions

Selection of Reference Genes for Gene Expression Analysis in Acacia melanoxylon under Different Conditions

Overexpression of a Ramie (Boehmaeria nivea L. Gaud) Group I WRKY Gene, BnWRKY49, Increases Drought Resistance in Arabidopsis thaliana

Genome-wide identification and comprehensive analysis of WRKY transcription factor family in safflower during drought stress

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