Progress in TILLING as a tool for functional genomics and improvement of crops

Chen, Liang; Hao, Liugen; Parry, M. A. J.; Phillips, Andrew L.; Hu, Yin Gang

doi:10.1111/jipb.12192

Cited by 100 publications

(51 citation statements)

References 118 publications

(182 reference statements)

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“…A second key development will be the advent of in silico TILLING in wheat. Traditionally, TILLING has relied on generating PCR-based amplicons from individual genes followed by a series of detection methods (reviewed in Chen et al, 2014). This throughput has been extended using NGS approaches to encompass multiple amplicons within one TILLING screen (Tsai et al, 2011).…”

Section: Exploiting Variationmentioning

confidence: 99%

Genomics as the key to unlocking the polyploid potential of wheat

2015

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1008I.1008II.1010III.1012IV.1013V.1014VI.1017VII.10191019References1019 Summary Polyploidy has played a central role in plant genome evolution and in the formation of new species such as tetraploid pasta wheat and hexaploid bread wheat. Until recently, the high sequence conservation between homoeologous genes, together with the large genome size of polyploid wheat, had hindered genomic analyses in this important crop species. In the past 5 yr, however, the advent of next‐generation sequencing has radically changed the wheat genomics landscape. Here, we review a series of advances in genomic resources and tools for functional genomics that are shifting the paradigm of what is possible in wheat molecular genetics and breeding. We discuss how understanding the relationship between homoeologues can inform approaches to modulate the response of quantitative traits in polyploid wheat; we also argue that functional redundancy has ‘locked up’ a wide range of phenotypic variation in wheat. We explore how genomics provides key tools to inform targeted manipulation of multiple homoeologues, thereby allowing researchers and plant breeders to unlock the full polyploid potential of wheat.

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Section: Exploiting Variationmentioning

confidence: 99%

Genomics as the key to unlocking the polyploid potential of wheat

2015

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“…In diesen Fällen müssen Züchter daher nach der Mutagenese Pflanzen mit der erwünschten Punktmutation im Ziel-Gen erst mit 24 GEBVs sind Bewertungen, die durch mathematische Berechnungen den einzelnen Haplotypen oder auch dem erwarteten Phänotyp der Pflanze zugeordnet werden können und die dann zur Grundlage der Zuchtentscheidungen herangezogen werden. Trotz seiner Nachteile ist TILLING ein wichtiges Werkzeug in der Pflanzenzüchtung geworden (Wang et al, 2012;Chen et al, 2014;Sharp und Dong, 2014). In der Entwicklung befinden sich weltweit salzresistente Tomaten, dürreresistente Sojabohnen, län-ger haltbare Erdbeeren, glutenfreier Weizen oder pilzresistente Gerste.…”

Section: Tillingunclassified

7. Themenbereich grüne Gentechnologie: Pflanzenzüchtung und Agrarwirtschaft

Müller‐Röber¹,

Marx-Stölting²

2015

Dritter Gentechnologiebericht

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“…Motif discovery tool 23 (Cartharius et al, 2005) make TILLING easier and simpler. It is expected that highthroughput TILLING method will become progressively popular, especially for agriculture, where there is so much useful knowledge to be gained from functional genomics and where no-transgenic methods are especially desirable (Chen et al, 2014). Eco-TILLING has also grown in popularity.…”

Section: Prospects and Future Consideration For Allele Miningmentioning

confidence: 99%

Allele Mining Strategies: Principles and Utilisation for Blast Resistance Genes in Rice (Oryza sativaL.)

Ashkani

Yusop²,

Shabanimofrad³

et al. 2015

Current Issues in Molecular Biology

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Allele mining is a promising way to dissect naturally occurring allelic variants of candidate genes with essential agronomic qualities. With the identification, isolation and characterisation of blast resistance genes in rice, it is now possible to dissect the actual allelic variants of these genes within an array of rice cultivars via allele mining. Multiple alleles from the complex locus serve as a reservoir of variation to generate functional genes. The routine sequence exchange is one of the main mechanisms of R gene evolution and development. Allele mining for resistance genes can be an important method to identify additional resistance alleles and new haplotypes along with the development of allele-specific markers for use in marker-assisted selection. Allele mining can be visualised as a vital link between effective utilisation of genetic and genomic resources in genomics-driven modern plant breeding. This review studies the actual concepts and potential of mining approaches for the discovery of alleles and their utilisation for blast resistance genes in rice. The details provided here will be important to provide the rice breeder with a worthwhile introduction to allele mining and its methodology for breakthrough discovery of fresh alleles hidden in hereditary diversity, which is vital for crop improvement.

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Progress in TILLING as a tool for functional genomics and improvement of crops

Cited by 100 publications

References 118 publications

Genomics as the key to unlocking the polyploid potential of wheat

Genomics as the key to unlocking the polyploid potential of wheat

7. Themenbereich grüne Gentechnologie: Pflanzenzüchtung und Agrarwirtschaft

Allele Mining Strategies: Principles and Utilisation for Blast Resistance Genes in Rice (Oryza sativaL.)

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