Progress of cationic gene delivery reagents for non-viral vector

Ma, Kai; Mi, Chunliu; Cao, Xiang-Xiang; Wang, Tianyun

doi:10.1007/s00253-020-11028-6

Cited by 40 publications

(23 citation statements)

References 90 publications

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“…In particular, synthetic or natural cationic polymers [ 7 , 8 ] containing primary, secondary, and/or tertiary amine groups can electrostatically bind and condense nucleic acids in the form of nanoscaled polyplexes [ 1 , 6 , 9 ]. The polyplex formation is an entropy-driven process based on ionic interaction between the cationic amino groups and the negatively charged phosphate groups of polyanionic nucleic acids [ 6 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Hydrophilic Random Cationic Copolymers as Polyplex-Formation Vectors for DNA

et al. 2022

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Research on the improvement and fabrication of polymeric systems as non-viral gene delivery carriers is required for their implementation in gene therapy. Random copolymers have not been extensively utilized for these purposes. In this regard, double hydrophilic poly[(2-(dimethylamino) ethyl methacrylate)-co-(oligo(ethylene glycol) methyl ether methacrylate] [P(DMAEMA-co-OEGMA)] random copolymers were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. The copolymers were further modified by quaternization of DMAEMA tertiary amine, producing the cationic P(QDMAEMA-co-OEGMA) derivatives. Fluorescence and ultraviolet–visible (UV–vis) spectroscopy revealed the efficient interaction of copolymers aggregates with linear DNAs of different lengths, forming polyplexes, with the quaternized copolymer aggregates exhibiting stronger binding affinity. Light scattering techniques evidenced the formation of polyplexes whose size, molar mass, and surface charge strongly depend on the N/P ratio (nitrogen (N) of the amine group of DMAEMA/QDMAEMA over phosphate (P) groups of DNA), DNA length, and length of the OEGMA chain. Polyplexes presented colloidal stability under physiological ionic strength as shown by dynamic light scattering. In vitro cytotoxicity of the empty nanocarriers was evaluated on HEK293 as a control cell line. P(DMAEMA-co-OEGMA) copolymer aggregates were further assessed for their biocompatibility on 4T1, MDA-MB-231, MCF-7, and T47D breast cancer cell lines presenting high cell viability rates.

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Section: Introductionmentioning

confidence: 99%

Hydrophilic Random Cationic Copolymers as Polyplex-Formation Vectors for DNA

et al. 2022

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“…24 Viral vectors are restricted in clinical applications due to their safety issues. Non-viral vectors, which have been widely developed and adopted due to their low virulence and non-immunogenicity, [25][26][27] include cationic liposomes, 28 polyethyleneimine (PEI) 29 and poly (β-amino ester) (PBAE). 30,31 Among non-viral vectors, PBAEs are a widely used type of cationic polymer gene carrier with excellent biocompatibility, good biodegradability, easy production, and low cost.…”

Section: Introductionmentioning

confidence: 99%

E7-Targeted Nanotherapeutics for Key HPV Afflicted Cervical Lesions by Employing CRISPR/Cas9 and Poly (Beta-Amino Ester)

Xiong¹,

Tan²,

Long³

et al. 2021

IJN

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“…Compared with common RNA DC vaccines, nanocarriers delivering tumor antigen RNA possess several advantages: (1) Avoiding the complex procedure of isolation of DCs; (2) Nanocarriers can effectively increase the stability of RNA; (3) Nanocarriers are more readily recognized and engulfed by DCs compared with free drugs. Cationic liposomes are one of the most studied carriers in gene therapy (Ma et al, 2021). We have previously successfully developed PEGylated liposome-polycation DNA complex (LPD) for siRNA and RNA delivery (Gao et al, 2011(Gao et al, , 2012(Gao et al, , 2013.…”

Section: Introductionmentioning

confidence: 99%

Tumor RNA-loaded nanoliposomes increases the anti-tumor immune response in colorectal cancer

Dai

Yin

et al. 2021

Drug Delivery

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Purpose: Tumor RNA vaccines can activate dendritic cells to generate systemic anti-tumor immune response. However, due to easily degraded of RNA, direct RNA vaccine is less effective. In this study, we optimized the method for preparing PEGylated liposom-polycationic DNA complex (LPD) nanoliposomes, increased encapsulate amount of total RNA derived from CT-26 colorectal cancer cells. Tumor RNA LPD nanoliposomes vaccines improved anti-tumor immune response ability of tumor RNA and can effectively promote anti-tumor therapeutic effect of oxaliplatin. Methods: Total tumor-derived RNA was extracted from colorectal cancer cells (CT-26 cells), and loaded to our optimized the LPD complex, resulting in the LPD nanoliposomes. We evaluated the characteristics (size, zeta potential, and stability), cytotoxicity, transfection ability, and tumor-growth inhibitory efficacy of LPD nanoliposomes. Results: The improved LPD nanoliposomes exhibited a spherical shape, RNA loading efficiency of 9.07%, the average size of 120.37 ± 2.949 nm and zeta potential was 3.34 ± 0.056 mV. Also, the improved LPD nanoliposomes showed high stability at 4 C, with a low toxicity and high cell transfection efficacy toward CT-26 colorectal cancer cells. Notably, the improved LPD nanoliposomes showed tumor growth inhibition by activating anti-tumor immune response in CT-26 colorectal cancer bearing mice, with mini side effects toward the normal organs of mice. Furthermore, the effect of the improved LPD nanoliposomes in combination with oxaliplatin can be better than that of oxaliplatin alone. Conclusion:The improved LPD nanoliposomes may serve as an effective vaccine to induce antitumor immunity, presenting a new treatment option for colorectal cancer.

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Progress of cationic gene delivery reagents for non-viral vector

Cited by 40 publications

References 90 publications

Hydrophilic Random Cationic Copolymers as Polyplex-Formation Vectors for DNA

Hydrophilic Random Cationic Copolymers as Polyplex-Formation Vectors for DNA

E7-Targeted Nanotherapeutics for Key HPV Afflicted Cervical Lesions by Employing CRISPR/Cas9 and Poly (Beta-Amino Ester)

Tumor RNA-loaded nanoliposomes increases the anti-tumor immune response in colorectal cancer

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