Progress on high-throughput sequencing and its applications in food web analysis

Xueqin, 王雪芹 Wang; Guanghua, 王光华 Wang; Fei, 乔飞 Qiao; Qikang, 高其康 Gao; Heong, K. L.; Zengrong, 祝增荣 Zhu; Jiaan, 程家安 Cheng

doi:10.5846/stxb201511162317

Acta Eco Sin

2017

DOI: 10.5846/stxb201511162317

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Progress on high-throughput sequencing and its applications in food web analysis

王雪芹 Wang Xueqin¹,

王光华 Wang Guanghua²,

乔飞 Qiao Fei³

et al.

Abstract: Ｖｏｌ．３７，Ｎｏ．８Ａｐｒ．，２０１７ｈｔｔｐ：／／ｗｗｗ．ｅｃｏｌｏｇｉｃａ．ｃｎ基金项目：国家科技支撑计划项目(２０１２ＢＡＤ１９Ｂ０１) ；浙江省自然科学基金项目(ＬＹ１６Ｃ１４０００２) ；科技部国家" ９７３" 基础重点研究发展规划项目 (２０１０ＣＢ１２６２００) 收稿日期：２０１５• １１• １６；网络出版日期：２０１６• １０• ２９ * 通讯作者Ｃｏｒｒｅｓｐｏｎｄｉｎｇａｕｔｈｏｒ．Ｅ•ｍａｉｌ：ｊａｃｈｅｎｇ＠ｚｊｕ．ｅｄｕ．ｃｎＤＯＩ：１０．５８４６／ｓｔｘｂ２０１５１１１６２３１７王雪芹，王光华，乔飞，高其康，ＫｏｎｇＬｕｅｎＨＥＯＮＧ，祝增荣，程家安．高通量测序及其在食物网解析中的应用进展．生态学报，２０１７，３７(８) ：２５３０• ２５３９．ＷａｎｇＸＱ，ＷａｎｇＧＨ，ＱｉａｏＦ，ＧａｏＱＫ，ＫｏｎｇＬｕｅｎＨＥＯＮＧ，ＺｈｕＺＲ，ＣｈｅｎｇＪＡ．Ｐｒｏｇｒｅｓｓｏｎｈｉｇｈ•ｔｈｒｏｕｇｈｐｕｔｓｅｑｕｅｎｃｉｎｇａｎｄｉｔｓａｐｐｌｉｃａｔｉｏｎｓｉｎｆｏｏｄｗｅｂ … Show more

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Vertebrate prey composition analysis of the Pallas’s cat (Otocolobus manul) in the Gongga Mountain Nature Reserve, based on fecal DNA

Zhao

Yang

et al. 2020

Mammalia

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Diet analysis is essential to fully understanding the biology of a species and its function within the ecosystem, and is also key in identifying food web interactions and the population dynamics of predators and prey. There is currently little information on the diet of small to mid-sized carnivores due to their elusive behavior. We used a DNA-based method to analyze the vertebrate prey of the Pallas’s cat (Otocolobus manul) in the Gongga Mountain Nature Reserve of southwestern China, a global biodiversity hotspot region. We confirmed 14 scat samples containing the Pallas’s cat DNA from 100 small carnivore scats collected at altitudes between 3200 and 4900 m, and then performed polymerase chain reaction (PCR) amplifications using primers targeting the vertebrate mitochondrial 12S rRNA gene with a blocking oligonucleotide specific to the predator sequence. We identified a total of 18 prey taxa, including nine mammals, eight birds and one fish. Our results show that pikas (in 79% of the scats) and birds (in 50% of the scats) are the predominant prey consumed by the Pallas’s cat in our investigation area. The most important finding in our study is that Cypriniformes species were found in the Pallas’s cat’s diet, exhibiting dietary diversification consistent with the accessible fauna of the surrounding environment. In order to increase the detectability of both typical and rare vertebrate prey items, we constructed a blocking primer to inhibit the amplification of the Pallas’s cat DNA.

show abstract

Vertebrate prey composition analysis of the Pallas’s cat (Otocolobus manul) in the Gongga Mountain Nature Reserve, based on fecal DNA

Zhao

Yang

et al. 2020

Mammalia

View full text Add to dashboard Cite

show abstract

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Progress on high-throughput sequencing and its applications in food web analysis

Cited by 1 publication

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Vertebrate prey composition analysis of the Pallas’s cat (Otocolobus manul) in the Gongga Mountain Nature Reserve, based on fecal DNA

Vertebrate prey composition analysis of the Pallas’s cat (Otocolobus manul) in the Gongga Mountain Nature Reserve, based on fecal DNA

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