Progression of Deoxynivalenol Concentrations in Spikes and Kernels of Winter Wheat Cultivars after Inoculation with Fusarium culmorum

Abstract

Progression of deoxynivalenol (DON) concentrations in spikes and kernels was studied in relation to Fusarium head blight (FHB) symptoms in five winter wheat cultivars, differing in resistance to FHB, after single floret inoculation with an aggressive isolate of Fusarium culmorum. After inoculation in field conditions the spikes were detached from the plant and kept in the greenhouse under controlled conditions. High concentrations of DON were detected in susceptible cultivars at an early stage of pathogenesis (7 days after inoculation). Over the whole examined 21-day period and also at maturity spikes contained more DON than kernels. While differences between cultivars in the accumulation of DON were highly expressed already 7 days after inoculation, differences in symptomatic reactions were not clear until day 21. Owing to the reported crucial role of DON at early stages of pathogenesis, the importance of appropriate timing of fungicide application is highly stressed.

Effect of Fusarium culmorum Tri Gene Transcription on Deoxynivalenol and D3G Levels in Two Different Barley Cultivars

“…The assessment as to the degree of F. culmorum infection in the two barley cultivars after inoculation under field conditions supports the selection of Chevron and Pedant as two contrasting genotypes for this study. The results of this experiment reconfirmed the high level of resistance to FHB in Chevron under field conditions (Miles et al 1987;Bollina et al 2011;Chrpov a et al 2011). The barley Pedant had previously been determined as susceptible to Fusarium infection (Chrpov a et al 2011).…”

“…Chrpová et al. () had detected a high concentration of DON in susceptible wheat cultivars at an early stage of pathogenesis (7 dpi with F. culmorum ). A large increase in the content of DON occurred between 14 and 21 dpi in these experiments.…”

“…The aim of this study was to specify the reactions of the pathogen in two barley cultivars differing in Fusarium resistance. The two cultivars had previously been reported as differing in the extent of their resistance to the pathogen under field conditions (Chrpov a et al 2011). We focused on determining pathogen response by monitoring changes in transcription of the Tri genes in relation to pathogen content and accumulation of mycotoxins (DON, D3G) in the host plants.…”

Impact de la date de récolte sur la teneur en désoxynivalénol (DON) des grains et la productivité de l'orge

“…Ainsi, sous inoculation avec F. graminearum, une accumulation de DON a e´teó bserve´e lorsque l'inoculation e´tait effectue´e du stade floraison jusqu'au stade paˆteux dur. Selon Chrpova et al (2006), le contenu en DON chez le ble´est de´ja`e´leve´7 jours apre`s l'inoculation et il y a une augmentation du contenu en DON jusqu'a`la maturite´de la ce´re´ale. Chez l'orge, une augmentation du contenu en DON a pu eˆtre observe´e entre le stade e´piaison et la maturite´du grain.…”

Evaluation of resistance to Fusarium head blight in wheat using different sources of inoculum

“…Four winter wheat cultivars differing in their responses to artificial infection with isolate B of Fusarium culmorum were selected on the basis of results of previous experiments (Chrpová et al 2006). The cultivar Arina could be characterized as moderately resistant, Saskia and Ebi as moderately susceptible (susceptible to DON accumulation) and Siria as susceptible to FHB.…”