Progression of experimental peri‐implantitis in guided bone regeneration and pristine bone: A preclinical in vivo study

Abstract

BackgroundGuided bone regeneration (GBR) is the most widely used technique for overcoming the deficiency of alveolar bone. However, the progression of peri‐implantitis in regenerative and pristine bone sites has not been fully investigated. The aim of this study is to compare experimental peri‐implantitis around implants placed in pristine bone and GBR sites.MethodsBilateral mandibular first molars were extracted from six beagle dogs, and standardized horizontal ridge defect was simultaneously created at predetermined site in unilateral mandible. After 8 weeks, guided bone regeneration procedure was conducted at the defect site. After 16 weeks, implants (ϕ 3.6×8.0 mm) were placed at both extracted sites. This study included 3 months of active breakdown and another 3 months of spontaneous progression period. Radiographs were taken at each phase and specimens were obtained for histological, immunohistochemical, and polarized light microscopic analysis.ResultsMarginal bone loss around implant did not show the significant differences between pristine bone and GBR sites during spontaneous progression period. In immunohistochemical analysis, inflammatory and immune‐related cells were predominantly detected in peri‐implantitis‒affected area rather than unaffected area. In the polarized light microscopic analysis, substantial reductions in the amount and thickness of collagen fibers were observed in peri‐implantitis‒affected area compared with unaffected tissues. However, there were no significant differences in histological, immunohistochemical, polarized light microscopic outcomes between pristine bone and GBR sites.ConclusionPrevious hard tissue grafting at the implant sites did not affect experimental peri‐implantitis and exhibited similar radiographic, histological, immunohistochemical, and polarized light microscopic outcomes compared with those of pristine bone sites.

Tissue changes around dental implants installed in alveolar ridge preservation sites: A 1‐year follow‐up randomized controlled clinical trial

“…The assumption that grafted sites may show a more pronounced MBL as a result of bacterial infection was addressed by an experimental periimplantitis study in dogs that found similar radiographic and histological results both in previously augmented and pristine sites after 6 months, suggesting that the grafted site hybrid bone composition does not affect its resorption pattern. 6 Similar experimental periimplantitis results were reported in sites grafted with xenograft (DBBM), autogenous bone, and nongrafted sites. 7 As mentioned before, the scientific literature is lacking in longterm clinical randomized control trials evaluating MBL and clinical parameters around implants placed in grafted sites.…”

The adjunctive effect of polydeoxyribonucleotide on bone formation in alveolar ridge preservation: A pre‐clinical in vivo study

“…Histological preparation procedures were conducted with reference to previous studies (J. J. Kim et al, 2019; Ko et al, 2023). Tissue blocks were obtained from mandibular segment specimens and demineralized in a 12.5% EDTA solution for 4 weeks.…”