To study the influence of slabs on the progressive collapse resistance of reinforced concrete (RC) frame structures, two 1:3 scaled substructures S1 (with slab) and B1 (without slab) were comprehensively tested. Both specimens had two spans, and each span had a length of 2 m. The width of the floor slab for S1 is 1 m for each side of the centerline frame beam. The two opposite sides of the specimens were completely fixed, and the remaining opposite sides were free. Two hydraulic jacks were applied at the top and bottom of the middle beam-column joint. At the beginning of the experiment, these two hydraulic jacks applied a pair of small balanced forces synchronously. Next, the bottom jack maintained the constant force, and at the same time, gradually increased the force of the top jack. Displacement controlled loading was used to simulate the failure of the middle column. By analyzing the load carrying capacities of S1 and B1, the influence of the slab on the progressive collapse resistance of the RC frame structures was discussed.