Progressive Effects of Sildenafil on Visual Processing in Rats

Østergaard, Freja Gam; Wade, Alex R.; Siebner, Hartwig R.; Christensen, Kenneth Vielsted; Laursen, Bettina

doi:10.1016/j.neuroscience.2020.06.033

Cited by 1 publication

(1 citation statement)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Coordinates were respective to bregma (for AP), the sagittal suture (for ML) and dura (for DV) - and were guided by (Paxinos and Watson, 1998). The animals were the same as the ones used in the study (Østergaard et al, 2020). Both the surgical details and the stimulation paradigms have been published in that article.…”

Section: Methodsmentioning

confidence: 99%

The effect of combined dexmedetomidine and isoflurane anaesthesia on visually evoked responses in rats – an electrophysiological study

Østergaard

Skoven

Laursen

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

The purpose of this study was to pilot an anaesthetic regime with the potential to be used for blood oxygenation level dependent functional magnetic resonance imaging (BOLD fMRI) of the visual system. We used electrophysiology to explore the effect of an anaesthesia regime, combining dexmedetomidine and isoflurane, on the visual system of female Sprague-Dawley rats. This paradigm is hypothesised to affect neural signalling less than other paradigms, and thus may be suitable for studies using BOLD fMRI. Electrodes were implanted bilaterally in the visual cortex (VC) and superior colliculus (SC) of 16 rats. Visual evoked potentials (VEPs) and steady-state VEPs (SSVEPs) were recorded during 1 Hz and 14 Hz light stimulation, respectively. As a control experiment an exploration of the wash-out of isoflurane was performed. The combined anaesthetic regime showed, that the visually evoked responses were almost completely abolished at the initiation of anaesthesia, but gradually recovered to baseline levels over time in the SC. In contrast, the evoked response in the VC only partially recovered, staying significantly below baseline condition. BOLD fMRI results from a previously published study supports this finding.

show abstract

Section: Methodsmentioning

confidence: 99%