Proinflammatory Cytokine IL-1β Stimulates IL-8 Synthesis in Mast Cells via a Leukotriene B4 Receptor 2-Linked Pathway, Contributing to Angiogenesis

Kim, Geun Young; Lee, Jin Wook; Ryu, Ho Cheol; Wei, Jun; Seong, Chu-Myong; Kim, Jae Hong

doi:10.4049/jimmunol.0901735

Cited by 91 publications

(74 citation statements)

References 59 publications

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“…Its expression is induced by vasoactive factors such as Ang II and platelet-derived growth factor (PDGF) and has been thought to be involved in proliferation and hypertrophy of VSMC (2,24). Expression of NOX1 was also reported in macrophages (25) and mast cells (26). In these cells, NOX1 was suggested to be involved in foam cell formation and interleukin (IL)-1β-induced IL-8 secretion contributing to angiogenesis, respectively.…”

Section: Identification Of Nox1mentioning

confidence: 93%

“…Kim et al, on the other hand, reported that NOX1-derived O 2 − is involved in IL-1β-induced activation of NF-κB and transcriptional activation of the IL-8 gene in mast cells. IL-1β was shown to induce NOX1 expression via leukotriene (LT) B 4 -BLT2 receptor signaling (26). Thus, there seems to be a positive feedback loop in which NF-κB activation by NOX1-derived O 2 − leads to further O 2 − production through induction of NOX1.…”

mentioning

confidence: 99%

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NOX/NADPH Oxidase, the Superoxide-Generating Enzyme: Its Transcriptional Regulation and Physiological Roles

Katsuyama

2010

J Pharmacol Sci

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Abstract. NADPH oxidase is a superoxide (O 2− )-generating enzyme first identified in phagocytes that shows bactericidal activities. It has been reported that O 2 − is also produced in an NADPHdependent manner in non-phagocytes. In the last decade, non-phagocyte-type NADPH oxidases have been identified, and the catalytic subunit NOX family has been found to be composed of five homologs, NOX1 to NOX5, and two related enzymes, DUOX1 and DUOX2. These NOX proteins have distinct features in dependency on other components for maximal enzymatic activity, tissue distribution, expressional regulation, and physiological functions. This review summarized the distinct characteristics of NOX family proteins, especially focusing on their functions and mechanisms of their expressional regulation.

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Section: Identification Of Nox1mentioning

confidence: 93%

mentioning

confidence: 99%

NOX/NADPH Oxidase, the Superoxide-Generating Enzyme: Its Transcriptional Regulation and Physiological Roles

Katsuyama

2010

J Pharmacol Sci

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“…On the basis of our previous observations showing that Nox functions downstream of BLT2, 17,18,22 we hypothesized that LPS might upregulate Nox1 through BLT2 in its signaling to IL-6 synthesis. Consistent with this notion, we observed that LPS-induced Nox1 gene expression and ROS generation were greatly attenuated by siRNA-mediated depletion of BLT2 in both peritoneal macrophages (Figures 4g and h) and Raw 264.7 cells (data not shown).…”

Section: Nox1-ros Signaling Functions Downstream Of Blt2 In Lpsinducementioning

confidence: 99%

MyD88–BLT2-dependent cascade contributes to LPS-induced interleukin-6 production in mouse macrophage

2015

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Endotoxic responses to bacterial lipopolysaccharide (LPS) are triggered by Toll-like receptor 4 (TLR4) and involve the production of inflammatory mediators, including interleukin-6 (IL-6), by macrophages. The detailed mechanism of IL-6 production by macrophages in response to LPS has remained unclear, however. We now show that LPS induces IL-6 synthesis in mouse peritoneal macrophages via the leukotriene B 4 receptor BLT2. Our results suggest that TLR4-MyD88 signaling functions upstream of BLT2 and that the generation of reactive oxygen species (ROS) by NADPH oxidase 1 (Nox1) and consequent activation of the transcription factor nuclear factor (NF)-κB function downstream of BLT2 in this response. These results suggest that a TLR4-MyD88-BLT2-Nox1-ROS-NF-κB pathway contributes to the synthesis of IL-6 in LPS-stimulated mouse macrophages.

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“…In addition, our results suggest that there is an interaction between BLT1 and NOX2 in HMC-1 cells stimulated with TvSP. There is increasing evidence showing possible signaling linkage between BLT and NOX (22,34,63). For example, it has been shown that BLT2-mediated NOX1 activation plays a key role in IL-8 production in human mast cells (HMC-1 cells) stimulated with IL-1␤ (22).…”

Section: Discussionmentioning

confidence: 99%

“…Upon activation by stimulation by chemotactic factors or chemokines, mast cells arrive in the inflamed tissues. These mast cells are further activated by the proinflammatory microenvironment, which ultimately leads to diverse cellular responses, such as adhesion to extracellular matrix proteins, neutrophils, or T cells, degranulation, and production of cytokines, chemokines, and/or cysteinyl leukotrienes (17)(18)(19)(20)(21)(22). Among these responses, degranulation plays a direct role in eliciting mast cell-mediated tissue inflammatory responses (23).…”

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confidence: 99%

SNAP23-Dependent Surface Translocation of Leukotriene B ₄ (LTB ₄ ) Receptor 1 Is Essential for NOX2-Mediated Exocytotic Degranulation in Human Mast Cells Induced by Trichomonas vaginalis-Secreted LTB ₄

et al. 2017

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Trichomonas vaginalis is a sexually transmitted parasite that causes vaginitis in women and itself secretes lipid mediator leukotriene B 4 (LTB 4 ). Mast cells are important effector cells of tissue inflammation during infection with parasites.Membrane-bridging SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes are critical for fusion during exocytosis. Although T. vaginalis-derived secretory products (TvSP) have been shown to induce exocytosis in mast cells, information regarding the signaling mechanisms between mast cell activation and TvSP is limited. In this study, we found that SNAP23-dependent surface trafficking of LTB 4 receptor 1 (BLT1) is required for nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2)-mediated exocytotic degranulation of mast cells induced by TvSP. First, stimulation with TvSP induced exocytotic degranulation and reactive oxygen species (ROS) generation in HMC-1 cells. Next, TvSP-induced ROS generation and exocytosis were strongly inhibited by transfection of BLT1 small interfering RNA (siRNA). TvSP induced trafficking of BLT1 from the cytosol to the plasma membrane. We also found that knockdown of SNAP23 abrogated TvSPinduced ROS generation, exocytosis, and surface trafficking of BLT1 in HMC-1 cells. By coimmunoprecipitation, there was a physical interaction between BLT1 and SNAP23 in TvSP-stimulated HMC-1 cells. Taken together, our results suggest that SNAP23-dependent surface trafficking of BLT1 is essential for exocytosis in human mast cells induced by T. vaginalis-secreted LTB 4 . Our data collectively demonstrate a novel regulatory mechanism for SNAP23-dependent mast cell activation of T. vaginalis-secreted LTB 4 involving surface trafficking of BLT1. These results can help to explain how the cross talk mechanism between parasite and host can govern deliberately tissue inflammatory responses.KEYWORDS Trichomonas vaginalis, LTB 4 , human mast cells, SNAP23, NOX2, BLT1, surface trafficking, exocytotic degranulation T richomonas vaginalis is a lumen-dwelling protozoan parasite with flagella, which infects the genitourinary tract in humans via sexual intercourse (1, 2). Of note, in women it causes vaginitis or cervicitis leading to inflammatory and allergic symptoms, including vaginal discharge with a foul odor and an increased number of leukocytes, and vulvovaginal pruritus (3-6). During the infection, tight junctional proteins between

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Proinflammatory Cytokine IL-1β Stimulates IL-8 Synthesis in Mast Cells via a Leukotriene B4 Receptor 2-Linked Pathway, Contributing to Angiogenesis

Abstract: Material

Cited by 91 publications

References 59 publications

NOX/NADPH Oxidase, the Superoxide-Generating Enzyme: Its Transcriptional Regulation and Physiological Roles

NOX/NADPH Oxidase, the Superoxide-Generating Enzyme: Its Transcriptional Regulation and Physiological Roles

MyD88–BLT2-dependent cascade contributes to LPS-induced interleukin-6 production in mouse macrophage

SNAP23-Dependent Surface Translocation of Leukotriene B ₄ (LTB ₄ ) Receptor 1 Is Essential for NOX2-Mediated Exocytotic Degranulation in Human Mast Cells Induced by Trichomonas vaginalis-Secreted LTB ₄

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Proinflammatory Cytokine IL-1β Stimulates IL-8 Synthesis in Mast Cells via a Leukotriene B4 Receptor 2-Linked Pathway, Contributing to Angiogenesis

Abstract: Material

Cited by 91 publications

References 59 publications

NOX/NADPH Oxidase, the Superoxide-Generating Enzyme: Its Transcriptional Regulation and Physiological Roles

NOX/NADPH Oxidase, the Superoxide-Generating Enzyme: Its Transcriptional Regulation and Physiological Roles

MyD88–BLT2-dependent cascade contributes to LPS-induced interleukin-6 production in mouse macrophage

SNAP23-Dependent Surface Translocation of Leukotriene B 4 (LTB 4 ) Receptor 1 Is Essential for NOX2-Mediated Exocytotic Degranulation in Human Mast Cells Induced by Trichomonas vaginalis-Secreted LTB 4

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SNAP23-Dependent Surface Translocation of Leukotriene B ₄ (LTB ₄ ) Receptor 1 Is Essential for NOX2-Mediated Exocytotic Degranulation in Human Mast Cells Induced by Trichomonas vaginalis-Secreted LTB ₄