Projection of FMRFamide-like neuropeptide-producing neurosecretory cells from silkworm brain into ventral nerve cord and retrocerebral complex

Abstract

Using immunostaining methodology, we traced the axonal projection of FMRFamide (Phe-Met-Arg-Phe-NH2)-like immunoreactive (LI) medial neurosecretory cells (MNCs) and lateral neurosecretory cells (LNCs) from the brain into the ventral nerve cord (VNC) and retrocerebral complex in Bombyx mori (L.) (Lepidoptera: Bombycidae). Of the seven pairs of FMRFamide-LI MNCs, one pair extended its axons from the brain pars intercerebralis into the VNC ipsilateral connective where they appeared to terminate. The axons of the remaining MNCs ran through decussation in the brain median region and contralateral nervi corporis cardiaci (NCC) I out of the brain, and eventually innervated the contralateral corpus cardiacum (CC). Axons from the single pair of FMRFamide-LI LNCs projected into the ipsilateral NCC II fused with NCC I without decussation in the brain, and finally terminated in the CC. These results suggest that transport of the FMRFamide-like neuropeptide from may be related to the modulation of functions such as gut contraction in MNCs terminating in the VNC, and regulation of production and/or secretion of specific hormones such as juvenile hormone in MNCs and LNCs terminating in the CC. Résumé-Utilisant une méthodologie immunostaining, nous avons retracé la projection axonaledes FMRFamide (Phe-Met-Arg-Phe-NH2)-like immunoréactive médial cellules neurosécrétrices (medical neurosecretory cells; MNCs) et de latérales cellules neurosécrétrices (Lateral neurosecretory cells; LNCs) à partir de le cerveau dans le corde nerveuse ventrale et retrocerebral complexes dans Bombyx mori (L.) (Lepidoptera: Bombycidae). les 7 paires de FMRFamide MNC, 1 paire axone du cerveau pars intercerebralis dans le VNC connectivites ipsilatérale où ils sont apparus á la fin. Les axones des MNC a couru dans la région du cerveau médian et controlatéral nervi corporis cardiaci (NCC), je hors du cerveau, et innervées du controlatéral corps cardiacum (CC). Les axones de la seule paire de FMRFamide LNC projetés dans le ipsilatérale NCC IIfusionné avec NCCI sans décussation dans le cerveau et se termine dans le CC. Ces résultats suggèrent que le transport des FMRFamide-comme le neuropeptide partir peut être liée à la modulation des fonctions telles que l'intestin contraction dans MNC de terminaison dans le VNC, et la régulation de la production et / ou la sécrétion de certaines hormones tels que juvenile hormone chez les MNC et les LNC se terminant par le CC.

Localization patterns of dopamine active transporter synthesizing cells during development of brine shrimp

“…The fixed samples were washed in 0.01 M phosphate‐buffered saline with 1% Triton X‐100 (1% PBST) at 4°C overnight. The samples were then dehydrated and rehydrated with a serial ethanol gradient (50 to 100% in 10 steps), washed with 1% PBST, and incubated with 10% normal serum overnight at 4°C (Kim et al., ; Kim, Song et al., 2015). The embryos were washed in 0.1 M Tris‐HCl buffer (pH 7.6) containing 1% Triton X‐100.…”

Distinctive Localization of Group 3 Late Embryogenesis Abundant Synthesizing Cells During Brine Shrimp Development

“…The fixed samples were then washed in 0.01 M phosphate‐buffered saline with 1% Triton X‐100 (1% PBST) at 4°C overnight. The samples were then dehydrated and rehydrated with a serial ethanol gradient (50–100% in 10% steps) and washed with 1% PBST, after which they were incubated with 10% normal serum overnight at 4°C (Kim et al., ). The embryos were washed in 0.1 M Tris–HCl buffer (pH 7.6) containing 1% Triton X‐100.…”