Projections of substance P, vasoactive intestinal peptide and tyrosine hydroxylase immunoreactive nerve fibres in the canine intestine, with special reference to the innervation of the circular muscle.

Furness, John B.; Lloyd, Kevin C K; Sternini, Catia; Walsh, John H.

doi:10.1679/aohc.53.129

Cited by 81 publications

(52 citation statements)

References 26 publications

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“…The circular muscle of the canine proximal colon is supplied by intrinsic excitatory and inhibitory motoneurones (Sanders & Smith 1986a,b;Furness et al, 1990). EFS is likely to activate these neural inputs simultaneously.…”

Section: Discussionmentioning

confidence: 99%

“…Immunohistochemical studies of the canine colon (Tange, 1983;Furness et al, 1990) (Furness et al, 1990) has since been shown to recognize both NKA and SP in guinea-pig enteric neurones (Shuttleworth et al, 1991). Therefore, it is possible that NKA is also contained in, and released from, neurones supplying the muscularis of the canine colon.…”

Section: Discussionmentioning

confidence: 99%

“…There is biochemical evidence suggesting that tachykinins are present in canine colonic muscles (Brodin et al, 1981;McDonald et al, 1990) and tachykinin-like immunoreactivity has been localized in enteric neurones that supply the muscularis (Tange, 1983;Furness et al, 1990). There is also autoradiographic evidence that tachykinin binding sites exist on colonic muscle cells (Mantyh et al, 1988) and it is likely that these receptors mediate the electrical and contractile responses to agonists such as substance P in these muscles (Huizinga et al, 1984;Keef et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of nitric oxide synthesis reveals non‐cholinergic excitatory neurotransmission in the canine proximal colon

Shuttleworth

Sanders

Keef

1993

British J Pharmacology

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1 Neuromuscular transmission in the circular muscle of the canine proximal colon was examined, in the presence and absence of nitric oxide synthase inhibitors, by use of mechanical and intracellular microelectrode recording techniques. 2 Electrical field stimulation (EFS; 0.1-20 HZ) produced frequency-dependent contractions of circular muscle strips which reached a maximum at 15 Hz. These reponses were enhanced by NG-monomethyl-Larginine (L-NMMA; 300 AM) and reduced by atropine (1 JAM). The effects of L-NMMA were reversed by L-arginine (3 mM). All responses to EFS were abolished by tetrodotoxin (1 AM).3 In the presence of atropine, phentolamine and propranolol (all at 1 AM; 'non-adrenergic, noncholingergic (NANC) conditions'), EFS evoked frequency-dependent inhibition of phasic contractions which reached a maximum at 5 Hz. At higher frequencies of EFS, inhibition diminished, and these responses were followed by post-stimulus excitation.4 Under NANC conditions and in the presence of L-NG-nitroarginine methyl ester (L-NAME; 200 JAM), EFS evoked contractions at frequencies of 5 Hz or greater. These contractions were reduced by co-incubation with L-arginine (2 mM) and abolished by tetrodotoxin (I iAM). 5In the presence of atropine (1 JAM), EFS (5-20 Hz) caused frequency-dependent inhibition of electrical slow waves. In the presence of L-NAME (100 JAM) and atropine, the inhibitory response to EFS was abolished and an increase in slow wave duration was seen at stimulation frequencies greater than 5 Hz. The effects of EFS on slow wave duration were abolished by tetrodotoxin (1 JiM). 6 Atropine-resistant contractions to EFS were enhanced by indomethacin (10 iAM) and reduced or abolished by the non-selective NK,/NK2 tachykinin receptor antagonist D-Pro2, D-Trp7'9 SP, and by the selective NK2 receptor antagonist MEN 10,376 (10 AM). 7 Exogenous tachykinins mimicked non-cholinergic excitatory electrical and mechanical responses. The rank order of potency for contraction was neurokinin A>neurokinin B>substance P, suggesting a predominance of the NK2 sub-type of tachykinin receptors on colonic smooth muscle cells. Low concentrations of neurokinin A also increased the amplitude and duration of electrical slow waves. 8 These results suggest that: (i) in previous studies, non-cholinergic excitatory responses were masked by the simultaneous release of NO; (ii) non-cholinergic excitatory responses occur throughout the period of stimulation and are not manifest only as 'rebound' excitation; (iii) one or more tachykinins, possibly, acting via NK2 receptors, may mediate non-cholinergic excitatory responses.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibition of nitric oxide synthesis reveals non‐cholinergic excitatory neurotransmission in the canine proximal colon

Shuttleworth

Sanders

Keef

1993

British J Pharmacology

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“…Cholinergic and cholinergic/substance Pcontaining motoneurons have been identified in the guinea-pig small intestine (Steele et (Furness et al 1990). In the present results, abolition of the ascending contraction by administration of atropine into the oral segment suggests that, in the canine small intestine, motoneurons for the ascending contraction elicited by stroking the mucosa are cholinergic and that the neuromuscular transmission is mediated by muscarinic receptors.…”

Section: Pathways For the Ascending Contractionmentioning

confidence: 99%

“…Cholinergic and/or substance P-containing excitatory motoneurons supplying the circular muscle have been identified histochemically (Furness, Lloyd, Sternini & Walsh, 1990;Steele et al 1991;Brookes et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Role of 5‐HT3 receptors in peristaltic reflex elicited by stroking the mucosa in the canine jejunum.

Neya

Mizutani

Yamasato

1993

The Journal of Physiology

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SUMMARY1. The role played by the 5-HT3 receptor, a serotonin subtype receptor, in peristaltic reflexes was studied in dogs first given ketamine, then anaesthetized with urethane (10 g kg-', i.v.) and a-chloralose (100 mg kg-', i.v.). The jejunal loop was partitioned into two segments with respect to blood supply. Drugs were infused intra-arterially into each segment.2. Stroking of the mucosa of the aboral and oral segments elicited an ascending contraction and a descending relaxation, respectively.3. The ascending contraction was concentration-dependently inhibited by treatment of the aboral segment with the 5-HT3 receptor antagonists ICS 205-930 and ondansetron (1-4 pmol min-' to 14 nmol min-' for both). The maximal inhibition was 49*5 and 69-3 %, respectively. The response was not affected by treatment of the oral segment with these drugs. The descending relaxation was inhibited by 51-4 and 60-8 %, respectively, by treatment of the oral segment with ICS 205-930 and ondansetron (1-4 nmol min-' for both).4. The ascending contraction was markedly inhibited by treatment of either segment with hexamethonium (140 nmol min-'). The response was abolished by treating both segments with hexamethonium and by treating the oral segment with atropine (14 nmol min-').5. These results suggest firstly that, in the canine jejunum, enteric neurons with 5-HT3 receptors play a role as sensory neurons or interneurons in the ascending excitatory and the descending inhibitory pathways of the peristaltic reflex elicited by stroking the mucosa, and secondly, that the ascending limb is composed of cholinergic interneurons and motoneurons.

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Classes of enteric nerve cells in the guinea-pig small intestine

2001

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Projections of substance P, vasoactive intestinal peptide and tyrosine hydroxylase immunoreactive nerve fibres in the canine intestine, with special reference to the innervation of the circular muscle.

Cited by 81 publications

References 26 publications

Inhibition of nitric oxide synthesis reveals non‐cholinergic excitatory neurotransmission in the canine proximal colon

Inhibition of nitric oxide synthesis reveals non‐cholinergic excitatory neurotransmission in the canine proximal colon

Role of 5‐HT3 receptors in peristaltic reflex elicited by stroking the mucosa in the canine jejunum.

Classes of enteric nerve cells in the guinea-pig small intestine

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