Prokaryotic diversity and dynamics in a full-scale municipal solid waste anaerobic reactor from start-up to steady-state conditions

Cardinali-Rezende, Juliana; Colturato, Luis Felipe Dornfeld Braga; Colturato, Thiago D.B.; Chartone-Souza, Edmar; Nascimento, Andréa M. A.; Sanz, J. L.

doi:10.1016/j.biortech.2012.05.136

Cited by 69 publications

(40 citation statements)

References 23 publications

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“…We suggest that the contrast was not attributable directly to bioreactor scale but likely to the semi-continuous operation and increased solids loading applied at full-scale promoting the development of a predominantly hydrogenotrophic methanogenic system. Indeed, hydrogenotrophic methanogensis is reported elsewhere in anaerobic digester systems treating high- and very-high solids wastes (Song et al, 2010; Garcia-Peña et al, 2011; Cardinali-Rezende et al, 2012). Thus, we propose that the dominance of acetoclastic methanogenesis, as is widely reported in laboratory-scale EGSB studies, may in fact be an artifact of artificially regular feeding regimes and readily biodegradable wastes applied in laboratory trials.…”

Section: Discussionmentioning

confidence: 90%

Bioreactor Scalability: Laboratory-Scale Bioreactor Design Influences Performance, Ecology, and Community Physiology in Expanded Granular Sludge Bed Bioreactors

et al. 2017

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Studies investigating the feasibility of new, or improved, biotechnologies, such as wastewater treatment digesters, inevitably start with laboratory-scale trials. However, it is rarely determined whether laboratory-scale results reflect full-scale performance or microbial ecology. The Expanded Granular Sludge Bed (EGSB) bioreactor, which is a high-rate anaerobic digester configuration, was used as a model to address that knowledge gap in this study. Two laboratory-scale idealizations of the EGSB—a one-dimensional and a three- dimensional scale-down of a full-scale design—were built and operated in triplicate under near-identical conditions to a full-scale EGSB. The laboratory-scale bioreactors were seeded using biomass obtained from the full-scale bioreactor, and, spent water from the distillation of whisky from maize was applied as substrate at both scales. Over 70 days, bioreactor performance, microbial ecology, and microbial community physiology were monitored at various depths in the sludge-beds using 16S rRNA gene sequencing (V4 region), specific methanogenic activity (SMA) assays, and a range of physical and chemical monitoring methods. SMA assays indicated dominance of the hydrogenotrophic pathway at full-scale whilst a more balanced activity profile developed during the laboratory-scale trials. At each scale, Methanobacterium was the dominant methanogenic genus present. Bioreactor performance overall was better at laboratory-scale than full-scale. We observed that bioreactor design at laboratory-scale significantly influenced spatial distribution of microbial community physiology and taxonomy in the bioreactor sludge-bed, with 1-D bioreactor types promoting stratification of each. In the 1-D laboratory bioreactors, increased abundance of Firmicutes was associated with both granule position in the sludge bed and increased activity against acetate and ethanol as substrates. We further observed that stratification in the sludge-bed in 1-D laboratory-scale bioreactors was associated with increased richness in the underlying microbial community at species (OTU) level and improved overall performance.

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Section: Discussionmentioning

confidence: 90%

Bioreactor Scalability: Laboratory-Scale Bioreactor Design Influences Performance, Ecology, and Community Physiology in Expanded Granular Sludge Bed Bioreactors

et al. 2017

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“…Archaeal vs. bacterial community ratios have been reported earlier at 4–8% in laboratory‐scale biogas reactors fed with a synthetic substrate based on cellulose and egg albumin (Sundh et al ., ), 6% in a reactor fed with fodder beet sludge (Klocke et al ., ), 6% in a household and garden waste reactor (Goberna et al ., ), and at 15% of the prokaryotic cells in a full‐scale municipal solid waste reactor (Cardinali‐Rezende et al ., ). In contrast, Montero et al .…”

Section: Discussionmentioning

confidence: 99%

454 pyrosequencing analyses of bacterial and archaeal richness in 21 full-scale biogas digesters

Sundberg

Al‐Soud

Larsson

et al. 2013

FEMS Microbiol Ecol

652

380

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The microbial community of 21 full-scale biogas reactors was examined using 454 pyrosequencing of 16S rRNA gene sequences. These reactors included seven (six mesophilic and one thermophilic) digesting sewage sludge (SS) and 14 (ten mesophilic and four thermophilic) codigesting (CD) various combinations of wastes from slaughterhouses, restaurants, households, etc. The pyrosequencing generated more than 160,000 sequences representing 11 phyla, 23 classes, and 95 genera of Bacteria and Archaea. The bacterial community was always both more abundant and more diverse than the archaeal community. At the phylum level, the foremost populations in the SS reactors included Actinobacteria, Proteobacteria, Chloroflexi, Spirochetes, and Euryarchaeota, while Firmicutes was the most prevalent in the CD reactors. The main bacterial class in all reactors was Clostridia. Acetoclastic methanogens were detected in the SS, but not in the CD reactors. Their absence suggests that methane formation from acetate takes place mainly via syntrophic acetate oxidation in the CD reactors. A principal component analysis of the communities at genus level revealed three clusters: SS reactors, mesophilic CD reactors (including one thermophilic CD and one SS), and thermophilic CD reactors. Thus, the microbial composition was mainly governed by the substrate differences and the process temperature.

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“…This group was found in studies of animal waste (Xia et al, 2012) and municipal solid waste anaerobic reactors (Cardinali-Rezende et al, 2012). WCHA1-57 was already found in an aquifer contaminated with hydrocarbons and chlorinated solvents undergoing intrinsic bioremediation, but it was never reported in anaerobic reactors (Dojka et al, 1998).…”

Section: Microbial Communities Of the Anaerobic Reactorsmentioning

confidence: 95%

Microbial community and performance of slaughterhouse wastewater treatment filters

Stets¹,

Etto²,

Galvão³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The performance of anaerobic filter bioreactors (AFs) is influenced by the composition of the substrate, support medium, and the microbial species present in the sludge. In this study, the efficiency of a slaughterhouse effluent treatment using three AFs containing different support media was tested, and the microbial diversity was investigated by amplified ribosomal DNA restriction analysis and 16S rRNA gene sequencing. The physicochemical analysis of the AF systems tested suggested their feasibility, with rates of chemical oxygen demand removal of 72 ± 8% in hydraulic retention times of 1 day. Analysis of pH, alkalinity, volatile acidity, total solids, total volatile solids, total Kjeldahl nitrogen, and the microbial community structures indicated high similarity among the three AFs. The composition of prokaryotic communities showed a prevalence of Proteobacteria (27.3%) and Bacteroidetes (18.4%) of the Bacteria domain and Methanomicrobiales (36.4%) and Methanosarcinales (35.3%) of the Archaea domain. Despite the high similarity of the microbial communities among the AFs, the reactor containing pieces of clay brick as a support medium presented the highest richness and diversity of bacterial and archaeal operational taxonomic units.

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Prokaryotic diversity and dynamics in a full-scale municipal solid waste anaerobic reactor from start-up to steady-state conditions

Cited by 69 publications

References 23 publications

Bioreactor Scalability: Laboratory-Scale Bioreactor Design Influences Performance, Ecology, and Community Physiology in Expanded Granular Sludge Bed Bioreactors

Bioreactor Scalability: Laboratory-Scale Bioreactor Design Influences Performance, Ecology, and Community Physiology in Expanded Granular Sludge Bed Bioreactors

454 pyrosequencing analyses of bacterial and archaeal richness in 21 full-scale biogas digesters

Microbial community and performance of slaughterhouse wastewater treatment filters

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