Prokaryotic Expression, Identification and Bioinformatics Analysis of the Mycobacterium tuberculosis Rv3807c Gene Encoding the Putative Enzyme Committed to Decaprenylphosphoryl-d-arabinose Synthesis

Abstract

Decaprenylphosphoryl-D-arabinofuranosyl (DPA), the immediate donor for the polymerized D-Araf residues of mycobacterial arabinan, is synthesized from 5-phosphoribose-1-diphosphate (PRPP) in three-step reactions.

Cyclipostins and Cyclophostin Analogues as Multitarget Inhibitors That Impair Growth of Mycobacterium abscessus

“…, Rv3807c) and MAB_3612 ( i.e. , Rv3265c) are possibly involved in the arabinogalactan biosynthesis; , and MAB_0944 ( i.e. , Rv0896) is a probable citrate synthase required for the tricarboxylic acid cycle …”

“…Interestingly, 9 out of the 39 identified proteins are annotated as essential enzymes in the M. tuberculosis genome (Table 3). 52,53 Among them, MAB_0172 (i.e., Rv3807c) and MAB_3612 (i.e., Rv3265c) are possibly involved in the arabinogalactan biosynthesis; 58,59 and MAB_0944 (i.e., Rv0896) is a probable citrate synthase required for the tricarboxylic acid cycle. 60 Of particular interest, the Ag85A/B/C proteins from M. tuberculosis have been recently validated as real targets of CyC 17 .…”

Three-component systems represent a common pathway for extracytoplasmic addition of pentofuranose sugars into bacterial glycans

“…Although many of the BLAST “hits” were uncharacterized proteins, the closest characterized homolog (31% sequence identity, 53% similarity) was found to be Mycobacterium tuberculosis decaprenylphosphoryl-5′-phosphoribosyltransferase (DPPRS). DPPRS is part of the pathway that generates cell wall–associated arabinogalactan and lipoarabinomannan in the extracellular space of mycobacteria ( 23 , 25 , 26 ) ( Fig. 1 B ).…”

“…Note that mycobacteria employ decaprenol for glycan and peptidoglycan biosynthesis, instead of the undecaprenol typical in gram-negative bacteria ( 28 ). In M. tuberculosis , Rv3807c encodes a phosphatase (unrelated to HAD-family PRPs) that removes the 5′-phosphate, producing decP-Rib f (DPR) ( 26 ). DPR is then epimerized by DprE1 (oxidase) and DprE2 (reductase) to make decP-arabinofuranose (DPA).…”

Two-Year Follow-up Study of Mycobacterium tuberculosis Antigen-Driven IFN-γ Responses and Macrophage sCD14 Levels After Tuberculosis Contact

“…The higher percentage of converters in the Household TB Contact group might have been a consequence of too short an interval between initial M.tb exposure and IGRA testing. The limited sensitivity of IGRA tests, preventing detection of weak mycobacteria-driven immune responses, justifies the search for new antigens with potent immunostimulatory activity and novel diagnostic methods for rapid identification of M.tb infections, in particular the most dangerous ones such as tuberculous meningitis [26][27][28]. A recent study by Fong et al [29] showed that IGRA conversions in serial testing remained a challenging task for clinicians and that using single cut-off point criteria for IGRA might lead to a significant number of false-positive results and overdiagnosis of LTBI.…”