Prokineticin signaling is required for the maintenance of a de novo population of c-KIT+ cells to sustain neuroblastoma progression

Lau, S-T; Hansford, Loen M.; Chan, WK; Chan, Gcf; Wan, Tmh; Wong, Kenneth K. Y.; Kaplan, David R.; Tam, Pkh; Ngan, Elly Sau-Wai

doi:10.1038/onc.2014.24

Cited by 18 publications

(22 citation statements)

References 29 publications

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“…There are papers showing that c-KIT is highly expressed or mutated in small cell lung cancer [7], leukemia cells [8], colon cancer [9] and neuroblastoma [10]. On the other hand, c-KIT expression is lost in breast cancer [11] and melanoma [12].…”

Section: Introductionmentioning

confidence: 99%

Loss of c-KIT expression in thyroid cancer cells

et al. 2017

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Papillary thyroid carcinoma is the most frequent histologic type of thyroid tumor. Few studies investigated the role of c-KIT expression in thyroid tumors, suggesting a role for this receptor and its ligand in differentiation and growth control of thyroid epithelium and a receptor loss following malignant transformation. We investigated and correlated c-KIT expression levels and two known markers of thyrocytes differentiation, PAX8 and TTF-1, in malignant and benign cytological thyroid samples. Moreover, we performed functional studies on human papillary thyroid carcinoma cell line to associated c-KIT expression to thyrocytes differentiation and tumor proliferation. c-KIT and PAX8 expression resulted higher in benign samples compared to the malignant ones, and the expression levels of these two genes were significantly correlated to each other. We also observed that c-KIT overexpression led to an increase of PAX8 expression level together with a decrease of proliferation. Furthermore, c-KIT overexpressing cells showed a regression of typical morphological features of malignancy. Taken together these results suggest that c-KIT could be involved in the differentiation of thyroid cells and in tumor progression.

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Section: Introductionmentioning

confidence: 99%

Loss of c-KIT expression in thyroid cancer cells

et al. 2017

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“…In addition, we identified protein-altering changes in the two independently isolated cup-13 alleles, and in eight of the nine independently isolated cup-11 alleles (Figure S2). Thus, CUP-13 is CUP-11, which is AEXR-2, a predicted seven-transmembrane domain, G protein-coupled receptor with homology to Prokineticin and Galanin Receptors in mammals (Floren et al 2000; Ngan and Tam 2008; Lau et al 2015). …”

Section: Resultsmentioning

confidence: 99%

Regulators of Lysosome Function and Dynamics inCaenorhabditis elegans

Gee¹,

Zamora²,

Horm

et al. 2017

G3 Genes|Genomes|Genetics

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Lysosomes, the major membrane-bound degradative organelles, have a multitude of functions in eukaryotic cells. Lysosomes are the terminal compartments in the endocytic pathway, though they display highly dynamic behaviors, fusing with each other and with late endosomes in the endocytic pathway, and with the plasma membrane during regulated exocytosis and for wound repair. After fusing with late endosomes, lysosomes are reformed from the resulting hybrid organelles through a process that involves budding of a nascent lysosome, extension of the nascent lysosome from the hybrid organelle, while remaining connected by a membrane bridge, and scission of the membrane bridge to release the newly formed lysosome. The newly formed lysosomes undergo cycles of homotypic fusion and fission reactions to form mature lysosomes. In this study, we used a forward genetic screen in Caenorhabditis elegans to identify six regulators of lysosome biology. We show that these proteins function in different steps of lysosome biology, regulating lysosome formation, lysosome fusion, and lysosome degradation.

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“…Receptor tyrosine kinase (KIT), a form of myeloid receptor that binds the stem cell factor, plays a major role in cancer occurrence [51]. Papers are showing that KIT is highly expressed in small cell lung cancer [52], leukemia cells [53], colon cancer [54], and neuroblastoma [55]. But KIT expression is lower in breast cancer [56] and melanoma [57].…”

Section: Discussionmentioning

confidence: 99%

Identification of Candidate Biomarkers Related to Diagnosis and Prognosis of Thyroid Cancer via Transcriptomic Profile Analysis

Li¹,

Jiang²,

Feng³

et al. 2020

Preprint

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BackgroundThe detection rate of thyroid cancer (TC) has been continuously improved due to the development of detection technology. Compared with other cancers, the gene profile plays a more prominent role in the diagnosis and treatment of TC. MethodsFour datasets from Gene Expression Omnibus (GEO) was used to perform transcriptomic profile analysis. The overlapping differentially expressed genes (DEGs) were analyzed by R package “limma” and “RobustRankAggreg”. Then the hub genes, which had a higher degree, were identified by the protein-protein interaction (PPI) network. Gene expression analysis between the TC and normal data, the disease-free survival (DFS) analysis of TC patients from Gene Expression Profiling Interactive Analysis (GEPIA) database, function analysis, and immunohistochemistry (IHC) were performed to verify the importance of the hub genes.ResultsA total of 80 DEGs (34 upregulated and 46 downregulated) were identified. Then FN1, TIMP1, ITGA2, and KIT were considered hub genes, which had a high degree of connectivity in the PPI network. GEPIA identified that FN1, TIMP1, and ITGA2 were upregulated, and KIT was downregulated. Upregulations of FN1 expression (P=0.024) and ITGA2 expression (P=0.029) and downregulation of KIT expression (P=0.012) increased risks of decreased DFS for patients. IHC showed that the expression of FN1, TIMP1, and ITGA2 protein were upregulated, while the expression of KIT protein was downregulated in the TC clinical specimens. Besides, five hub genes were enriched in the PI3K-Akt signaling pathway and ECM-receptor interaction.ConclusionsIn summary, these hub genes were potential biomarkers of diagnosis and prognosis of TC.

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Prokineticin signaling is required for the maintenance of a de novo population of c-KIT+ cells to sustain neuroblastoma progression

Cited by 18 publications

References 29 publications

Loss of c-KIT expression in thyroid cancer cells

Loss of c-KIT expression in thyroid cancer cells

Regulators of Lysosome Function and Dynamics inCaenorhabditis elegans

Identification of Candidate Biomarkers Related to Diagnosis and Prognosis of Thyroid Cancer via Transcriptomic Profile Analysis

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