Prolactin and Angiogenesis: Biological Implications of Microheterogeneity

Muralidhar, K.; Lee, Jaeok

doi:10.5772/54318

Cited by 2 publications

(2 citation statements)

References 82 publications

(86 reference statements)

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“…3C). SVF cells cultured in NF-ELR mostly upregulated (79%) the release of anti-angiogenic factors, including Insulin Growth Factor Binding Protein-3 (IGFBP-3) [32], prolactin [33], angiostatin [34] or vasohibin [35] (Fig. 3C).…”

Section: In Vitro Culture Of Elrs-based Constructsmentioning

confidence: 99%

Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel

et al. 2017

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The control of the in vivo vascularization of engineered tissue substitutes is essential in order to obtain either a rapid induction or a complete inhibition of the process (e.g. in muscles and hyaline-cartilage, respectively). Among the several polymers available, Elastin-Like Recombinamers (ELRs)-based hydrogel stands out as a promising material for tissue engineering thanks to its viscoelastic properties, non-toxicity, and non-immunogenicity. In this study, we aimed to modulate the high angiogenic potential of adipose tissue-derived stromal vascular fraction (SVF) cells, predominantly composed of endothelial/mural and mesenchymal cells, by simply varying the cell adhesion properties of ELRs-hydrogels. Human SVF cells, embedded in RGD-REDVbioactivated or unmodified ELRs-hydrogels, were implanted in rat subcutaneous pockets either immediately or upon 5-day-culture in perfusion-bioreactors. Perfusionbased culture enhanced the endothelial cell cord-like-organization and the release of pro-angiogenic factors in functionalized constructs. While in vivo vascularization and host cell infiltration within the bioactivated gels were highly enhanced, the two processes were strongly inhibited in non-functionalized hydrogels up to 28 days. ELRsbased hydrogels showed a great potential to determine the successful integration of engineered substitutes thanks to their capacity to finely control the angiogenic/inflammation process at the recipient site, even in presence of SVF cells.

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Section: In Vitro Culture Of Elrs-based Constructsmentioning

confidence: 99%

Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel

et al. 2017

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“…PRL, also known as luteotropic hormone, secreted from the pituitary gland, has been reported to be aberrantly activated in lung tumors, especially in neuroendocrine tumors, 36 suggesting the enrichment of alterations in PRL pathway might be a shared feature of tumors arise from endocrine and nervous systems. In addition, PRL may play several other active roles in tumor development by stimulating angiogenesis 37 and activating pro-proliferation and anti-apoptotic pathways, including but not limited to PI3K/Akt/mTOR, MEK1/2/ERK1/2, p38MAPK, STAT3, and p53. [38][39][40] In conclusion, our study interrogating 72 matched CSF and plasma samples, demonstrate that CSF is a superior liquid biopsy median than plasma for genomic analysis of LM, evident by associating with a significantly higher detection rate and MaxAF.…”

Section: Discussionmentioning

confidence: 99%

Unique genomic profiles obtained from cerebrospinal fluid cell-free DNA of non-small cell lung cancer patients with leptomeningeal metastases

Ying

Ding

et al. 2018

Cancer Biology & Therapy

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Background: Leptomeningeal metastases (LM), associated with poor prognosis, are frequent complications of advanced non-small cell lung cancer (NSCLC) patients, especially in patients with epidermal growth factor receptor (EGFR) mutations. Due to limited access to leptomeningeal lesions, the mutational landscape of LM has not been comprehensively investigated in large cohorts and the underlining biology of LM remains elusive. Some studies have explored the potential of cerebrospinal fluid (CSF) in reflecting the molecular profile of LM but with limited number of patients enrolled. Methods: In this study, we performed capture-based targeted sequencing using a panel consisting of 168 lung cancer-related genes on matched CSF and plasma samples from 72 advanced NSCLC patients with confirmed LM to interrogate the potential of CSF as a source of liquid biopsy. Results: We revealed a rate of detection of 81.5% and 62.5% for CSF and plasma, respectively (p = 0.008). The maximum allelic fraction (MaxAF) was also significantly higher in CSF (43.6% vs. 4.6%) (p < 0.001). CSF, harboring a unique genomic profile by having a significant number of CSF-specific mutations, primarily copy number variations, is superior to plasma in reflecting the mutational profile of LM. Further pathway enrichment analysis revealed that most of CSF-specific mutations participated in pathways relevant to the tumorigenesis and the development of metastases. Moreover, our data also revealed that TP53 loss of heterozygosity (LOH) predominantly existed in CSF (p < 0.001). Conclusions: Collectively, we demonstrated that CSF provides a more comprehensive profile of LM than plasma in a large cohort, thus can be used as an alternative source of liquid biopsy for LM patients.

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Prolactin and Angiogenesis: Biological Implications of Microheterogeneity

Cited by 2 publications

References 82 publications

Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel

Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel

Unique genomic profiles obtained from cerebrospinal fluid cell-free DNA of non-small cell lung cancer patients with leptomeningeal metastases

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