Prolactin exerts hematopoietic growth-promoting effects in vivo and partially counteracts myelosuppression by azidothymidine

Woody, Mary Alice; Li, Renhao; Sun, Rui; Tian, Zhi Gang; Henry, Michelle; Richards, Susan; Raziuddin, Arati; Longo, Dan L.; Murphy, William J.

doi:10.1016/s0301-472x(99)00019-3

Cited by 48 publications

(31 citation statements)

References 41 publications

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“…Given the increase in erythropoiesis in these animals, many of these cells are likely to be erythroid precursors -a suggestion consistent with their morphology. An increase in erythropoiesis in response to PRL is consistent with the findings of studies in which PRL was administered to mature mice (Woody et al 1999) and work analyzing bone marrow stromal production of PRL (Bellone et al 1997). Our work, however, is the first to show distinct effects of the two forms of PRL, effects in rats and effects on the developing hematopoietic system.…”

Section: Figuresupporting

confidence: 79%

Administration of unmodified prolactin (U-PRL) and a molecular mimic of phosphorylated prolactin (PP-PRL) during rat pregnancy provides evidence that the U-PRL:PP-PRL ratio is crucial to the normal development of pup tissues

Yang

Kuo²,

Liu³

et al. 2001

Journal of Endocrinology

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During rat pregnancy initial high concentrations of prolactin (PRL) decline by about day 9, concomitant with an increase in the ratio of unmodified to phosphorylated PRL. The physiological significance of both the decline in total PRL and the change in ratio of the two PRLs is unknown. To test the importance of each, either unmodified PRL (U-PRL) or a molecular mimic of phosphorylated PRL (PP-PRL) were continuously administered to rats throughout pregnancy. A dose of 6 µg/24 h resulted in circulating concentrations of 50 ng/ml of each administered PRL and had little effect on the pregnancy itself.

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Section: Figuresupporting

confidence: 79%

Administration of unmodified prolactin (U-PRL) and a molecular mimic of phosphorylated prolactin (PP-PRL) during rat pregnancy provides evidence that the U-PRL:PP-PRL ratio is crucial to the normal development of pup tissues

Yang

Kuo²,

Liu³

et al. 2001

Journal of Endocrinology

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“…rhPRL was generously provided by William J. Murphy from NCI-Frederick, NIH, Frederick, MD, and its quality and usage have been verified in previous studies (32,33). Mice were injected i.p.…”

Section: Methodsmentioning

confidence: 99%

Human Prolactin Promotes Human Secondary Immunoglobulin Response in Human/SCID Mouse Chimeras

Zhang

Sun

Tian

2007

Clin Vaccine Immunol

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“…Recombinant human prolactin was endotoxinfree and supplied as a gift by Dr. William J. Murphy, NCI-FCRDC, Frederick, MD, which has been used in their studies [22][23][24][25]. The IL-2-dependent NK cell line NK-92, established from a patient with rapidly progressive non-Hodgkin's lymophoma [26], was purchased from ATCC and maintained in a-MEM (Hyclone) medium containing100U/ml IL-2 and other necessary components as described [26].…”

Section: Cell Culture and Proliferation Assaymentioning

confidence: 99%

“…PRL also increased the proliferating response and cytotoxicity of human, murine or rat NK cells to sensitive or insensitive tumor independently or synergistically with IL-2 in vitro [17][18][19][20]. We previously reported that PRL improved the hematopoiesis and lymphocyte development in syngeneic bone marrow transplantation [21][22][23][24], and exerted anti-tumor effects on tumor-bearing mice in vivo [25]. However, thus far there has been little evidence indicating that PRL can directly affect NK cell function through prolactin receptor on human NK cells.…”

Section: Introductionmentioning

confidence: 99%

Expression of prolactin receptor and response to prolactin stimulation of human NK cell lines

Sun

Wei

et al. 2004

Cell Res

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We have previously shown a critical role of prolactin (PRL) during maturation and anti-tumor effects of murine natural killer (NK) cells in vitro and in vivo. We extended that study by exploring the ability of human NK cell lines (NK-92 and YT cell) to express PRL receptor (PRL-R) and to respond to PRL stimulation in vitro. Both human NK cell lines constitutively expressed PRL-R on membrane and mRNA transcripts, NK-92 cells contained higher level of PRL-R than YT cells, which correlated to the enhanced capacity of the cells to proliferate and to lyse target cells in response to PRL stimulation in the presence of trace amount of IL-2 or IL-15 in vitro. Two differences between IL-2 and IL-15 in functioning on human NK cells were for the first time observed. PRL synergized with IL-15 to improve proliferation of NK cells in a dose-dependent manner without double peak manifesting like IL-2. Although PRL enhanced the cytotoxicity of IL-2 or IL-15 activated NK cells, it exerted the function through up-regulating gene expression of perforin without influence of FasL in IL-2-stimulated NK cells, while in IL-15-stimulated NK cells, PRL did the function through up-regulating gene expression of both perforin and FasL but not IFNγ. PRL increased expressions of IL-2Rα on membrane and of IL-2 mRNA in cells, indicating that PRL up-regulated NK cell function by improving positive feedback between IL-2 and IL-2R. The similar results were also observed in network between IL-15 and IL-15R. These data indicate a potential role of PRL in human NK cell modulation.

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Prolactin exerts hematopoietic growth-promoting effects in vivo and partially counteracts myelosuppression by azidothymidine

Cited by 48 publications

References 41 publications

Administration of unmodified prolactin (U-PRL) and a molecular mimic of phosphorylated prolactin (PP-PRL) during rat pregnancy provides evidence that the U-PRL:PP-PRL ratio is crucial to the normal development of pup tissues

Administration of unmodified prolactin (U-PRL) and a molecular mimic of phosphorylated prolactin (PP-PRL) during rat pregnancy provides evidence that the U-PRL:PP-PRL ratio is crucial to the normal development of pup tissues

Human Prolactin Promotes Human Secondary Immunoglobulin Response in Human/SCID Mouse Chimeras

Expression of prolactin receptor and response to prolactin stimulation of human NK cell lines

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