Prolactin-Independent Induction of α-Lactalbumin Gene Expression in Mammary Gland Explants from Pregnant Balb/C Mice

Warner, B.; Janssens, P. A.; Nicholas, Kevin R.

doi:10.1006/bbrc.1993.1918

Cited by 8 publications

(3 citation statements)

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“…The titration of total RNA and hybridization to a cDNA probe for the ovine /Mactoglobulin gene revealed a linear response up to 1 /ig. RNA, which is similar to that, reported previously for studies on milk protein gene expression in the tammar wallaby (Collet et al .1991), rat (Rosen et al 1989) and mouse (Warner et al 1993).…”

Section: Discussionsupporting

confidence: 89%

Hormone-dependent expression of the ovine β-lactoglobulin gene

Osborne

Howell

et al. 1995

Journal of Dairy Research

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SUMMARYThe minimal hormonal requirements for inducing the ovine β-lactoglobulin gene have been investigated using mammary gland explants from ewes in the first half of pregnancy. Quantification of β-lactoglobulin mRNA showed that a combination of insulin, Cortisol and prolactin was required to stimulate the expression of the gene and that this response could not be enhanced by the addition of oestrogen and thyroid hormone to the culture medium. Explants cultured in the presence of insulin, Cortisol and prolactin also demonstrated the capacity to synthesize the protein. Progesterone did not inhibit the induction of the gene, which is consistent with the increase in β-lactoglobulin mRNA observed in vivo in the mammary gland during the final 2 months of pregnancy when the circulating level of progesterone is elevated.

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Section: Discussionsupporting

confidence: 89%

Hormone-dependent expression of the ovine β-lactoglobulin gene

Osborne

Howell

et al. 1995

Journal of Dairy Research

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“…It is worth highlighting that the stimulatory effect of PRL on LALBA synthesis occurs in concert with other lactogenic hormones, specifically GC, INS, and T3. The synthesis of CSN2 and lipid in mammary explants from midpregnant mice and rats required PRL [50,51]. Whereas LALBA could also be synthesized by explants cultured in a medium supplemented with only INS and GC, its synthesis was delayed by 24 h in the absence of PRL [52].…”

Section: Prolactinmentioning

confidence: 95%

“…Transgenic mice overexpressing human LALBA from a construct containing a 0.77 kb 5' fragment expressed the exogenous gene and protein at levels 14-fold greater than those for endogenous LALBA, without any effect on milk lactose content [195]. Transgenic sows bearing a bovine LALBA construct that included 2 kb of upstream sequence produced 20-50% more milk that had a higher milk lactose content and lower total solids, protein, and fat concentration than control animals [50]. This positive effect of bovine LALBA on milk composition was still apparent in the second lactation, where sows produced twice the amount of bovine LALBA in colostrum and milk versus during their first lactation [196,197].…”

Section: Lessons From Transgenic Animals Carrying An Exogenous Lalba Sequencementioning

confidence: 99%

A Comparative Review of the Extrinsic and Intrinsic Factors Regulating Lactose Synthesis

Sadovnikova

García

Hovey

2021

J Mammary Gland Biol Neoplasia

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Milk is critical for the survival of all mammalian offspring, where its production by a mammary gland is also positively associated with its lactose concentration. A clearer understanding of the factors that regulate lactose synthesis stands to direct strategies for improving neonatal health while also highlighting opportunities to manipulate and improve milk production and composition. In this review we draw a cross-species comparison of the extra- and intramammary factors that regulate lactose synthesis, with a special focus on humans, dairy animals, and rodents. We outline the various factors known to influence lactose synthesis including diet, hormones, and substrate supply, as well as the intracellular molecular and genetic mechanisms. We also discuss the strengths and limitations of various in vivo and in vitro systems for the study of lactose synthesis, which remains an important research gap.

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