Prolactin-induced accumulation of casein mRNA in mouse mammary explants: A selective role of glucocorticoid

Nagaiah, K.; Bolander, Franklyn F.; Nicholas, Kevin R.; Takemoto, Tai‐ichiro; Topper, Yale J.

doi:10.1016/0006-291x(81)90851-2

Cited by 36 publications

(14 citation statements)

References 11 publications

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“…En effet, l'action insulino-mimétique d'anticorps antirécep-teurs de l'insuline atteste que l'effet est bien de type insulinique (Nicholas et ). Par opposition, le fait qu'une stimulation intense par l'insuline n'est obtenue qu'à concentration très élevée (Houdebine et al, 1985b) Nagarah et al, 1981 ;Houdebine et al, 1985b) a fait apparaître la souris plutôt que la lapine comme une exception. Un examen plus attentif a permis de montrer par ailleurs que chez la rate, une dépendance franche vis-à-vis des glucocorticôides apparaît dès lors que les animaux sur lesquels est prélevé le tissu mammaire sont surrénalectomisés .…”

unclassified

Contrôle hormonal du développement et de l'activité de la glande mammaire

Houdebiné¹

1986

Reprod. Nutr. Dévelop.

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Contrôle hormonal du développement et de l'activité de la glande mammaire

Houdebiné¹

1986

Reprod. Nutr. Dévelop.

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“…IGF-I, insulin, and growth hormone are described in SFB composition (REYNOLDS et al, 1990;SINGH, ARMSTRONG, 1997). Insulin, hydrocortisone, and prolactin are required in rodents for induction of CSN2 gene expression NAGAIAH et al, 1981;KULSKI et al, 1983). However, insulin is essential for CSN2 transcription, but not for transcript stabilization (CHOMCZYNSKI et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

In vitro culture of ovine mammary gland cells expressing beta-lactoglobulin and beta-casein

Giassetti

Barros

Mendes

et al. 2017

Braz. J. Vet. Res. Anim. Sci.

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The expression of milk proteins in vitro is essential to exploit the mammary gland cells as a biological model. Enzymatic tissue disaggregation has been widely used to establish mammary cell culture, but its effect in long-term ovine mammary cell culture is not completely elucidated. This study aimed at comparing mechanical/enzymatic and mechanical dissociation methods to establish ovine mammary cell culture. We compared cellular differentiation induced by lactating ewe serum or fetal bovine serum based on the gene expression levels of milk proteins (beta-lactoglobulin, alpha s1-casein, and betacasein). Mechanically dissociated cells were positive immunostaining for cytokeratin 8.13, such as mammary epithelial cells. These cells are responsible for milk protein expression and they are low immunostaining for vimentin, mesenchymal marker. Mechanical/enzymatic dissociation cells were positive for vimentin. The fastest cell growth (cell/hour) was observed in the mechanical dissociation group cultured with 10% fetal bovine serum medium. Mechanically and mechanically/enzymatically derived cells were able to express beta-casein and beta-lactoglobulin, but not alpha s1-casein. The relative expression of beta-lactoglobulin was not affected by the tissue dissociation method or culture media, beta-casein relative expression was down regulated in mechanically dissociated cells cultured in the presence of lactating ewe serum, (P = 0.019). Beta-casein relative expression was also down regulated in mechanically/enzymatically dissociated cells cultured with fetal bovine serum (P = 0.021). In the present conditions, we conclude that mechanical dissociation followed by culture with 10% of fetal bovine serum was the most efficient method to induce milk proteins' mRNA expression by ovine mammary epithelial cells in vitro. Keywords: Mammary cell culture. Milk proteins. Beta-casein. Ovine. ResumoA expressão in vitro de proteínas do leite é essencial para explorar as células da glândula mamária como um modelo biológico. A desagregação tecidual via enzimática é amplamente utilizada para o estabelecimento cultivo de células mamárias. No entanto, seu efeito a longo prazo no cultivo de células da glândula mamária ovina ainda não é bem elucidado. Este estudo tem como objetivo comparar dois métodos de dissociação tecidual, mecânico/enzimático e mecânico, para estabelecer cultivo celular de glândula mamária ovina. A indução da diferenciação celular, por adição de soro de ovelha lactante ou soro fetal bovino, foi avaliada pelos níveis de expressão de proteínas do leite (beta-lactoglobulina, alpha s1-caseína e beta-caseína). Células mecanicamente dissociadas foram positivamente marcadas para a presença de citoqueratina 8.13, marcador para células epiteliais mamárias. Essas células são as responsáveis pela produção das proteínas do leite e são pouco marcadas para a presença de vimentina, marcador para células de origem mesenquimal. Já as células obtidas da dissociação mecânica/ enzimática foram positivamente marcadas para presença de vi...

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“…Such a conclusion agrees well with the role of these hormones in normal mammary gland differen¬ tiation. In murine mammary expiants, insulin (Chomczynski, Qasba & Topper, 1984) and prolac¬ tin (Nagaiah, Bolander, Nicholas et al 1981) stimulate casein mRNA accumulation; in the case of prolactin, this effect appears to be due to an increase in both transcription and mRNA stability (Guyette, Matusik & Rosen, 1979). Progesterone also affects transcription, although this effect may either be positive (Cato, Miksicek, Schütz et al 1986) or negative (Rosen, O'Neal, McHugh & Comstock, 1978), depending upon the gene.…”

Section: Discussionmentioning

confidence: 99%

Developmental and hormonal regulation of a mouse mammary tumour virus glycoprotein in normal mouse mammary epithelium

Bolander

Blackstone²

1990

Journal of Molecular Endocrinology

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A radioimmunoassay was developed and validated for the major glycoprotein (gp58) of the mouse mammary tumour virus (MMTV). Using this assay, the expression of gp58 during pregnancy and lactation was found to parallel that for MMTV RNA. In particular, there was a very rapid induction in late pregnancy and a decline in late lactation, although some residual expression persisted well into involution. In cultures of normal mouse mammary tissue, induction of gp58 occurred after a 24-h lag period and began to reach a plateau after 3 days. Both the insulin and prolactin dose-response curves for gp58 resembled those for MMTV RNA; in contrast, the effects of steroid hormones on gp58 and MMTV RNA were disparate. Although progesterone stimulated the RNA, it only slightly increased gp58 levels; however, the presence of cortisol greatly augmented this stimulation, despite the inability of cortisol to induce RNA at physiological concentrations. These results suggest that insulin, prolactin and progesterone act primarily at the level of RNA accumulation in normal mammary epithelium, while cortisol affects some more distal event.

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Prolactin-induced accumulation of casein mRNA in mouse mammary explants: A selective role of glucocorticoid

Cited by 36 publications

References 11 publications

Contrôle hormonal du développement et de l'activité de la glande mammaire

Contrôle hormonal du développement et de l'activité de la glande mammaire

In vitro culture of ovine mammary gland cells expressing beta-lactoglobulin and beta-casein

Developmental and hormonal regulation of a mouse mammary tumour virus glycoprotein in normal mouse mammary epithelium

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