Prolactin-Releasing Activity in Methanol Extracts of Human Plasma: Problems with the Bioassay of This Activity*

Halpern, Barry; Malarkey, William B.

doi:10.1210/jcem-52-2-199

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…The active substance emerged at a concentration of 0.025 M ammonium formate (fraction No. [9][10][11][12]. The active portion on CM-cellulose (CM-52) ion exchange chromatography was then lyophilized and applied on a TSK-LS4 10 C-18 reverse-phase HPLC column.…”

Section: (4) Isolation Of Prolactin-releasing Factor In Porcine Hypotmentioning

confidence: 99%

A New in vitro Assay System for Prolactin‐Releasing Activity and its Application for Demonstration of Porcine Hypothalamic Prolactin‐Releasing Factor (PRF)

Nomura

Miyamoto

Hasegawa

et al. 1985

Asia-Oceania Journal of Obstetrics and Gynaecology

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A new sensitive and reproducible assay system for prolactinreleasing activity (PRA) using a rat pituitary cell culture system was established. After pretreatment with bromocriptine (1.3 x M) in culture medium for 24 h r on the 4th day in culture, the 6 hr addition of thyrotropin releasing hormone (TRH) to culture medium increased prolactin secretion in a dose-dependent manner at concentrations of TRH ranging from 3.2 x lo-" M t o 2.0 x M; maximal prolactin response was 270% of the control value.By using this new assay system for PRA, prolactin-releasing factor (PRF) in porcine hypothalami was isolated and identified. On Sephadex G-25 gel-chromatography of acid extracts of porcine hypothalami, PRA was found in the small peptide portion. These fractions of PRA were further subjected to CM-cellulose (CM-52) ion exchange chromatography, followed by reverse-phase HPLC. PRA obtained by HPLC chromatography was neutralized by anti-TRH antibody. It was concluded that TRH is the main PRF in porcine hypothalamic extracts.

show abstract

Section: (4) Isolation Of Prolactin-releasing Factor In Porcine Hypotmentioning

confidence: 99%

A New in vitro Assay System for Prolactin‐Releasing Activity and its Application for Demonstration of Porcine Hypothalamic Prolactin‐Releasing Factor (PRF)

Nomura

Miyamoto

Hasegawa

et al. 1985

Asia-Oceania Journal of Obstetrics and Gynaecology

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Variation of serum prolactin-releasing activity in women with the galactorrhea-amenorrhea syndrome after bromocriptine treatment

Takahashi

Minato

Ikeno

et al. 1983

American Journal of Obstetrics and Gynecology

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Prolactin-Releasing Activity in Methanol Extracts of Human Plasma: Problems with the Bioassay of This Activity*

Cited by 2 publications

References 8 publications

A New in vitro Assay System for Prolactin‐Releasing Activity and its Application for Demonstration of Porcine Hypothalamic Prolactin‐Releasing Factor (PRF)

A New in vitro Assay System for Prolactin‐Releasing Activity and its Application for Demonstration of Porcine Hypothalamic Prolactin‐Releasing Factor (PRF)

Variation of serum prolactin-releasing activity in women with the galactorrhea-amenorrhea syndrome after bromocriptine treatment

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