Proliferating bovine intramuscular preadipocyte cells synthesize leptin

Yonekura, Shinichi; Tokutake, Yukako; Hirota, Shohei; Rose, Michael T.; Katoh, K.; Aso, Hisashi

doi:10.1016/j.domaniend.2013.03.004

Cited by 6 publications

(7 citation statements)

References 32 publications

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“…To elucidate this hypothesis, we analyzed the abundance of vascular growth factor VEGF-A in tissue homogenates of the claw fat pads. Synthesis of VEGF-A during cell differentiation has been shown in bovine intramuscular preadipocytes (Yonekura et al, 2013). In mature adipocytes, VEGF-A mRNA expression varies in different fat depots (Yamada et al, 2010), which is explained by different adipocyte sizes.…”

mentioning

confidence: 96%

Short communication: Adipocyte sizes in the digital fat pad and their relationship to body condition in dairy cows

Hiss-Pesch

Weise

Heitkönig

et al. 2019

Journal of Dairy Science

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Lameness and body condition are closely related. Recent studies have shown that cows with low body condition score (BCS) have a greater risk for developing lameness than cows with higher BCS. Among other reasons, this relationship might be related to the reduced thickness of the digital fat cushion in lean cows. The digital cushion is not a homogeneous structure but consists of different fat pads and connective tissue. We hypothesized that either high or low BCS will result in concordant adipocyte sizes in the fat pads of the digital cushion and subcutaneous tailhead fat irrespective of the localization of the latter. Right front claws were collected from 18 Holstein Friesian cows. Cows were selected according to their BCS: 9 cows with BCS <3 (low BCS) and 9 cows with BCS ≥3 (high BCS). After dissecting the horn capsule of the lateral claw, samples of the axial and abaxial fat pads were prepared for histomorphological examinations (adipocyte size measurement) and protein abundance of vascular endothelial growth factor A (VEGF-A) via Western blotting. In addition, fat samples were excised from the tailhead of all cows and used for the same purposes. Adipocyte size in tailhead fat was greater in high-BCS than in low-BCS cows. Similar differences between the BCS groups were apparent for adipocytes from the axial fat pad, although adipocytes in tailhead fat were larger than those in the digital cushion. In contrast to that in the axial fat pad and tailhead fat, adipocyte size in the abaxial fat pad was similar in cows from both BCS groups. A relationship between adipocyte size and VEGF-A protein was only confirmed for the axial fat pad, not for the other fat depots. When comparing BCS groups, differences in VEGF-A protein abundance between high-BCS and low-BCS cows were also limited to the axial fat pad, being absent in tailhead fat and the abaxial fat pad. Taken together, our results show that the fat pads from the digital cushion should not be considered uniform adipose tissue locations but rather discrete units reacting differently to fat mobilization.

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mentioning

confidence: 96%

Short communication: Adipocyte sizes in the digital fat pad and their relationship to body condition in dairy cows

Hiss-Pesch

Weise

Heitkönig

et al. 2019

Journal of Dairy Science

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“…VEGFA is a key factor in the regulation of angiogenesis in adipose tissue [ 86 ]. Fat deposition is angiogenesis dependent, and the quality of adipose tissue can be regulated by the vascular system [ 87 ]. Angiogenesis of subcutaneous adipose tissue promotes the proliferation of adipose cells, thus improving the lipid storage capacity of adipose tissue, which in turn can prevent metabolic disorders of the body [ 88 ].…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome Scanning for Selection Signatures Reveals Candidate Genes Associated with Growth and Tail Length in Sheep

Li,

Jin,

Wang

et al. 2024

Animals

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Compared to Chinese indigenous sheep, Western sheep have rapid growth rate, larger physique, and higher meat yield. These excellent Western sheep were introduced into China for crossbreeding to expedite the enhancement of production performance and mutton quality in local breeds. Here, we investigated population genetic structure and genome-wide selection signatures among the Chinese indigenous sheep and the introduced sheep based on whole-genome resequencing data. The PCA, N-J tree and ADMIXTURE results showed significant genetic difference between Chinese indigenous sheep and introduced sheep. The nucleotide diversity (π) and linkage disequilibrium (LD) decay results indicated that the genomic diversity of introduced breeds were lower. Then, Fst & π ratio, XP-EHH, and de-correlated composite of multiple signals (DCMS) methods were used to detect the selection signals. The results showed that we identified important candidate genes related to growth rate and body size in the introduced breeds. Selected genes with stronger selection signatures are associated with growth rate (CRADD), embryonic development (BVES, LIN28B, and WNT11), body size (HMGA2, MSRB3, and PTCH1), muscle development and fat metabolism (MSTN, PDE3A, LGALS12, GGPS1, and SAR1B), wool color (ASIP), and hair development (KRT71, KRT74, and IRF2BP2). Thus, these genes have the potential to serve as candidate genes for enhancing the growth traits of Chinese indigenous sheep. We also identified tail-length trait-related candidate genes (HOXB13, LIN28A, PAX3, and VEGFA) in Chinese long-tailed breeds. Among these genes, HOXB13 is the main candidate gene for sheep tail length phenotype. LIN28A, PAX3, and VEGFA are related to embryonic development and angiogenesis, so these genes may be candidate genes for sheep tail type traits. This study will serve as a foundation for further genetic improvement of Chinese indigenous sheep and as a reference for studies related to growth and development of sheep.

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“…Recently, we demonstrated that BIP cells can synthesize leptin during the preadipocyte stage (Yonekura et al, 2013). In the present study, we showed that completely differentiated BIP cells expressed leptin mRNA, as reported previously in rodent adipocytes and primary cultures of bovine adipocytes (Rentsch and Chiesi, 1996; Soliman et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…The concentration of the isolated mRNA was determined by measuring the optical density at 260 nm, and the purity of the RNA was determined based on the ratio of the absorbance at 260 nm relative to the absorbance at 280 nm. RT-PCR was performed as described previously (Yonekura et al, 2013). PCR amplification of the cDNA samples was conducted using the following bovine leptin gene primer pair: 5′-GTGCCCATCCGCAAGGTCCA-3′ and 5′-TCAGCACCCGGGACTGAGGT-3′ (450-bp product, GenBank accession no.…”

Section: Methodsmentioning

confidence: 99%

“…BIP cells have the capacity to proliferate and differentiate into mature adipocytes; therefore, they may be useful for studying the mechanisms of adipocyte differentiation or adipogenic hormone expression/secretion in ruminants. Recently, we demonstrated that BIP cells can synthesize leptin during the preadipocyte stage and that a cytoplasmic leptin pool may be present in these preadipocytes (Yonekura et al, 2013). In the present study, we focused on cytosolic leptin protein and investigated its expression in BIP cells during differentiation.…”

Section: Introductionmentioning

confidence: 99%

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Dexamethasone and Acetate Modulate Cytoplasmic Leptin in Bovine Preadipocytes

Yonekura

Hirota

Tokutake

et al. 2014

Asian Australas. J. Anim. Sci

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Hormonal and nutrient signals regulate leptin synthesis and secretion. In rodents, leptin is stored in cytosolic pools of adipocytes. However, not much information is available regarding the regulation of intracellular leptin in ruminants. Recently, we demonstrated that leptin mRNA was expressed in bovine intramuscular preadipocyte cells (BIP cells) and that a cytoplasmic leptin pool may be present in preadipocytes. In the present study, we investigated the expression of cytoplasmic leptin protein in BIP cells during differentiation as well as the effects of various factors added to the differentiation medium on its expression in BIP cells. Leptin mRNA expression was observed only at 6 and 8 days after adipogenic induction, whereas the cytoplasmic leptin concentration was the highest on day 0 and decreased gradually thereafter. Cytoplasmic leptin was detected at 6 and 8 days after adipogenic induction, but not at 4 days after adipogenic induction. The cytoplasmic leptin concentration was reduced in BIP cells at 4 days after treatment with dexamethasone, whereas cytoplasmic leptin was not observed at 8 days after treatment. In contrast, acetate significantly enhanced the cytoplasmic leptin concentration in BIP cells at 8 days after treatment, although acetate alone did not induce adipocyte differentiation in BIP cells. These results suggest that dexamethasone and acetate modulate the cytoplasmic leptin concentration in bovine preadipocytes.

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Proliferating bovine intramuscular preadipocyte cells synthesize leptin

Cited by 6 publications

References 32 publications

Short communication: Adipocyte sizes in the digital fat pad and their relationship to body condition in dairy cows

Short communication: Adipocyte sizes in the digital fat pad and their relationship to body condition in dairy cows

Whole-Genome Scanning for Selection Signatures Reveals Candidate Genes Associated with Growth and Tail Length in Sheep

Dexamethasone and Acetate Modulate Cytoplasmic Leptin in Bovine Preadipocytes

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