Proliferating Cell Nuclear Antigen (PCNA) Expression in Tooth Primordia in the Field Vole ( Microtus agrestis , Rodentia)

Matulová, Petra; Witter, Kirsti; Mı́šek, Ivan

doi:10.1080/713713536

Cited by 6 publications

(15 citation statements)

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“…The location of proliferating cell populations within the developing teeth of the vole has been previously confirmed by immunohistochemical detection of proliferation marker PCNA (Matulova et al, 2002). The apoptotic nature of the condensed nuclear bodies has been previously confirmed by TUNEL staining (Matalova et al, 2004b).…”

Section: Mitosis and Apoptosis Evaluationmentioning

confidence: 62%

“…The primary enamel knot is characterised as an area of non-dividing cells in the centre of the tooth bud to bell and is believed to be essential for the transition from the bud to cap stage. They express a wide range of regulatory genes including Shh, Fgf, Wnt and Bmp family members (for overview, see http://bite-it.helsinki.fi/ 2003), connected to a complex regulatory network that may determine the position of secondary enamel knots and further the localisation of cusps in the prospective tooth TUNEL method, respectively (Matalova et al, 2004b;Matulova et al, 2002). PCNA was detected in cycling cells in both dental epithelium and mesenchyme, but not in antemolar vestiges, enamel knots and differentiated ameloblasts and odontoblasts (Matulova et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…They express a wide range of regulatory genes including Shh, Fgf, Wnt and Bmp family members (for overview, see http://bite-it.helsinki.fi/ 2003), connected to a complex regulatory network that may determine the position of secondary enamel knots and further the localisation of cusps in the prospective tooth TUNEL method, respectively (Matalova et al, 2004b;Matulova et al, 2002). PCNA was detected in cycling cells in both dental epithelium and mesenchyme, but not in antemolar vestiges, enamel knots and differentiated ameloblasts and odontoblasts (Matulova et al, 2002). Apoptosis was observed in PCNA negative areas in the middle axis of the tooth bud and later in the enamel knot region and in the stalk.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have been focused on possible roles of cell proliferation and apoptosis during tooth morphogenesis (Lesot et al, 1996;Shigemura et al, 1999;Peterkova et al, 2003). Proliferation was investigated using complementary approaches all along tooth development (Casasco et al, 1992;Ruch, 1995;Lesot et al, 1996;Shigemura et al, 1999;Matulova et al, 2002). Mitosis in both dental epithelium and mesenchyme is involved in the development of the cervical loop in molars, together with the mesial elongation of the first molar (Lesot et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Proliferation and apoptosis during the odontogenesis of the field vole have been assessed by detection of the proliferation marker Proliferating Cell Nuclear Antigen (PCNA) (Matulova et al, 2002) and by TUNEL test (Matalova et al, 2004b), respectively. However, neither the spatial distribution of proliferating and apoptotic cells within the tooth primordia nor their relationship with tooth morphogenesis is known in detail.…”

Section: Introductionmentioning

confidence: 99%

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Proliferation and apoptosis in early molar morphogenesis - voles as models in odontogenesis

Setkova¹,

Lesot²,

Matalová³

et al. 2006

Int. J. Dev. Biol.

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Proliferation and apoptosis play crucial roles in the development of multicellular organisms. Their precise balance is necessary for tissue homeostasis throughout life. The developing dentition is a suitable model to study proliferation and apoptosis during embryogenesis, but the corresponding studies have been carried out principally in the mouse. The present study aimed to examine proliferation and apoptosis in the vole (Microtus sp., Rodentia) during the early morphogenesis of the first upper molar and compare it to what is known from the mouse. To this end, apoptosis and proliferation were investigated using histology and computer-aided 3D reconstruction. Mitoses accumulated predominantly in the developing cervical loop. Apoptosis during early odontogenesis showed highly specific spatio-temporal patterns in the dental epithelium. Apoptotic bodies were localised in non-dividing cell populations. They accumulated in the same places as described in the mouse: antemolar vestiges (ED 12.5 -15.5), enamel knot (ED 14.5 -15.5), stalk and palatally along the whole first molar tooth germ longitudinal axis (ED 15 -15.5). Early tooth development in the field vole, including the distribution of apoptosis and mitosis, is very similar to that reported in the mouse, with the exception of the antemolar region. The microtine antemolar vestige is preserved longer than the murine one. It is conceivable that additional distinct differences in morphogenetic processes appear later in tooth development.

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Section: Mitosis and Apoptosis Evaluationmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Proliferation and apoptosis in early molar morphogenesis - voles as models in odontogenesis

Setkova¹,

Lesot²,

Matalová³

et al. 2006

Int. J. Dev. Biol.

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Miniaturization: How many cells are needed to build a tooth?

Larionova

Lesot

Huysseune

2021

Developmental Dynamics

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Background: Organs that develop early in life, and are replaced by a larger version as the animal grows, often represent a miniature version of the adult organ. Teeth constituting the first functional dentition in small-sized teleost fish, such as medaka (Oryzias latipes), are examples of such miniature organs. With a dentin cone as small as the size of one human cell, or even smaller, these teeth raise the question how many dentin-producing cells (odontoblasts) are required to build such a tooth, and whether this number can be as little as one.Results: Based on detailed observations with transmission electron microscopy (TEM) and TEM-based 3D-reconstructions, we show that only one mesenchymal cell qualifies as a true odontoblast. A second mesenchymal cell potentially participates in dentin formation, but only at a late stage of tooth development. Moreover, the fate of these cells appears to be specified very early during tooth development. Conclusions: Our observations indicate that in this system, one single odontoblast fulfills roles normally exerted by a large and communicating cell population. First-generation teeth in medaka thus provide an exciting model to study integration of multiple functions into a single cell.

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Relationship between vestibular lamina, dental lamina, and the developing oral vestibule in the upper jaw of the field vole (Microtus agrestis, Rodentia)

Witter

Pavlíková

Matulová

et al. 2005

Journal of Morphology

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Formation of the oral vestibule is ignored in most studies on tooth development, although dental and vestibular lamina are closely related to each other. Knowledge about morphogenetic processes shaping the oral vestibule is missing almost completely. The aim of this study was to assess the developmental relationship between dental and vestibular lamina as well as formation of the oral vestibule in the upper jaw of the field vole (Microtus agrestis), a small rodent representing an attractive model species for comparative dental studies. Three-dimensional reconstruction revealed that the upper vestibular lamina of the vole joins the antemolar part of the diastemal dental lamina, similar to mouse. Later, this lamina complex regresses and the vestibular lamina is separated from the molar epithelium. Participation of the vestibular lamina in dental lamina formation, as hypothesized for mouse, therefore remains unclear. Except for increased apoptosis in the regressing diastemal dental lamina, spatial segregation of mitoses or apoptoses could be detected neither in the jaw arch epithelium nor in the adjacent mesenchyme. Therefore, in contrast to tooth primordia, apoptosis and mitosis seem to play a minor role in shaping of the upper oral vestibule. The buccal vestibule develops secondarily, probably in consequence of general growth of the head and localized differentiation of cells.

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Proliferating Cell Nuclear Antigen (PCNA) Expression in Tooth Primordia in the Field Vole ( Microtus agrestis , Rodentia)

Cited by 6 publications

References 0 publications

Proliferation and apoptosis in early molar morphogenesis - voles as models in odontogenesis

Proliferation and apoptosis in early molar morphogenesis - voles as models in odontogenesis

Miniaturization: How many cells are needed to build a tooth?

Relationship between vestibular lamina, dental lamina, and the developing oral vestibule in the upper jaw of the field vole (Microtus agrestis, Rodentia)

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