Proliferation and differentiation of thyrotrophs in the pars distalis of the rat pituitary gland during the fetal and postnatal period

Taniguchi, Y.; Yasutaka, Satoru; Kominami, Rieko; Shinohara, Harumichi

doi:10.1007/s004290100161

Cited by 26 publications

(22 citation statements)

References 26 publications

(36 reference statements)

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“…Gulyás et al (1993) reported the postnatal development of ACTH, GH and PRL cells in female rats using [ 3 H]dT autoradiography and immunocytochemistry. We have studied quantitatively the development of the pituitary through fetal and postnatal periods using the BrdU method combined with immunocytochemistry (Taniguchi et al 2000(Taniguchi et al , 2001a(Taniguchi et al , 2001b(Taniguchi et al , 2001c.…”

Section: Proliferation Of Rat Pituitary Cells During Ontogenesismentioning

confidence: 99%

Proliferation and differentiation of rat anterior pituitary cells

Taniguchi

Yasutaka

Kominami

et al. 2002

Anatomy and Embryology

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Studies on the proliferation and differentiation of the cells in the rat anterior pituitary were reviewed. The mitotic rate of anterior pituitary is low in the control adult animal, but it increased by stimulation, such as by ablation of the target organ. A high mitotic rate was also reported during ontogenesis of the pituitary. Concomitant with this augmented mitosis, the number of those cells that are double-labeled with the marker of proliferation and the antibody to pituitary hormones increased as well. The percentage of these double-labeled cells in all the proliferating cells is less than 10%, suggesting that about 1/10 of the proliferating cells are involved in producing pituitary cells. This percentage for GH cells is 30-40% at most, suggesting very active production of them. The percentage of the double-labeled cell in all the hormone-producing cells is within 10% in all cell-types of the pituitary, including GH cells. When the proliferation is detected by a more sensitive method, this percentage increased to 20-40%, suggesting that the self-mitosis of the pituitary cells contributes considerably to their proliferation at a certain period during their ontogenesis.

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Section: Proliferation Of Rat Pituitary Cells During Ontogenesismentioning

confidence: 99%

Proliferation and differentiation of rat anterior pituitary cells

Taniguchi

Yasutaka

Kominami

et al. 2002

Anatomy and Embryology

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“…We have studied, by quantitative immunocytochemistry, the proliferation of corticotrophs and thyrotrophs during late fetal and early postnatal periods, and present data indicating that mitosis contributes considerably to the proliferation of these cell types (Taniguchi et al 2000(Taniguchi et al , 2001a(Taniguchi et al , 2001b. In this study we examined the proliferation and differentiation of somatotrophs and mammotrophs quantitatively by double immunostaining of pituitary sections using anti-bromodeoxyuridine (BrdU), and anti-rat GH or anti-rat PRL.…”

Section: Introductionmentioning

confidence: 98%

Proliferation and differentiation of pituitary somatotrophs and mammotrophs during late fetal and postnatal periods

Taniguchi

Yasutaka

Kominami

et al. 2001

Anatomy and Embryology

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Proliferation of somatotrophs and mammotrophs in the rat pituitary during late fetal and postnatal periods up to 4 weeks after birth was quantitatively studied with the double immunostaining of bromodeoxyuridine and the hormones produced by them. Somatotrophs were first detected in 18.5-day fetuses and rapidly increased in number throughout the periods studied. The cells labeled with both anti-BrdU and anti-GH were few in number until shortly before birth and then increased conspicuously during the first 10 days after birth. Mammotrophs were detected at gestational day 19.5 but they were few until the second week after birth, when their number began to increase rapidly. The percentage of the number of the cells double-labeled with both anti-BrdU and anti-GH to all somatotrophs was 8.3% at the most. This was about the same as that of corticotrophs during the late fetal period and that of thyrotrophs in the early postnatal period. In contrast, the percentage of double-labeled cells to all mammotrophs was 3.8% as a maximum, which is lower than the values for somatotrophs, corticotrophs, or thyrotrophs, indicating a smaller contribution of mitosis to mammotroph proliferation. It is possible that this smaller contribution is compensated for by transdifferentiation of cells committed to become the somatotroph lineage. However, coexistence of GH and PRL was not observed in the present material.

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“…After performing triple imunofluorescent staining (for b and a subunit and proliferative marker), it was shown that fully differentiated thyrotropes can also replicate. Taniguchi et al (2001) established that in rat fetuses differentiated immunocytochemically recognized TSH cells are mitotically active, and their proliferation contributes to increased numbers, especially during the neonatal period. Considering the absolute number of immunocytochemically labeled TSH cells, our results are generally consistent with those of Taniguchi and coworkers, although the number of TSH cells expressed per pituitary was lower in their experiment (4,900 vs. 3,300 TSH cells per pituitary, respectively).…”

Section: Discussionmentioning

confidence: 98%

“…Further differentiation from precursor cells enables detection of TSH mRNA on day 15 of rat gestation (Brown et al, 2000), and immunocytochemically recognized thyrotropes can be observed in 16.5-to 17.5-day-old fetuses. Thyrotropes were few in 17.5 day rat fetuses but increased thereafter, particularly during the 2nd week after birth (Taniguchi et al, 2001).…”

Section: Introductionmentioning

confidence: 98%

Unbiased stereological estimation of the rat fetal pituitary volume and of the total number and volume of TSH cells after maternal dexamethasone application

Manojlović‐Stojanoski¹,

Nestorović²,

Ristić³

et al. 2010

Microscopy Res & Technique

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Glucocorticoids have an inhibitory influence on proliferation activity of the pituitary cells while stimulating apoptosis. Therefore, it was hypothesized that the synthetic glucocorticoid, dexamethasone (DX), has an inhibitory influence on the number of thyroid-stimulating hormone (TSH) cells during fetal development. The effects of maternal administration of DX on stereological parameters of TSH cells, and TSH serum concentration were investigated in 21-day-old rat fetuses. On day 16 of pregnancy, the experimental dams received 1.0 mg DX/kg b.w. subcutaneously, followed by 0.5 mg DX/kg b.w./day on days 17 and 18 of gestation. The control gravid females received the same volume of saline vehicle. TSH cells were stained immunocytochemically by the peroxidase-antiperoxidase (PAP) method. The fetal pituitary volumes were estimated using Cavalieri's principle. A physical disector counting technique in combination with the fractionator sampling method was used for estimation of pituitary TSH cell number. Cell and nuclear volumes were measured with a planar rotator. Maternal DX application was found to cause a significant decrease of pituitary volume and number of TSH cells per pituitary in 21-day-old fetuses in comparison with the control fetuses. TSH cell number expressed per body weight unit declined significantly after maternal DX administration. These results indicate an inhibitory DX influence on proliferative activity of precursors and likely differentiated TSH cells and increased apoptotic prevalence. The histological appearance, volume of TSH cells and TSH serum concentration suggest intensive synthetic activity in TSH cells of DX exposed fetuses.

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Proliferation and differentiation of thyrotrophs in the pars distalis of the rat pituitary gland during the fetal and postnatal period

Cited by 26 publications

References 26 publications

Proliferation and differentiation of rat anterior pituitary cells

Proliferation and differentiation of rat anterior pituitary cells

Proliferation and differentiation of pituitary somatotrophs and mammotrophs during late fetal and postnatal periods

Unbiased stereological estimation of the rat fetal pituitary volume and of the total number and volume of TSH cells after maternal dexamethasone application

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