Proliferation and extracellular matrix synthesis of smooth muscle cells cultured from human coronary atherosclerotic and restenotic lesions

Abstract

These data indicate that increased smooth muscle cell proliferation contributes to coronary restenosis in humans and support the concept that the extracellular matrix synthesis of adult smooth muscle cells is important to lesion formation.

Non Insulin Dependent Diabetes in Sand Rat (Psammomys obesus) and Production of Collagen in Cultured Aortic Smooth Muscle Cells. Influence of Insulin

“…Karim et al. [33] have noted equally an increase of mRNA coding for 01 (I) zl (III) chains after 30 days of experimental angioplasty. Katsuda et al [32] have shown the coexistence of the two phenotypes in the intimal lesions, but type I collagen would stimulate the SMC migration, t38] During the development of the atherosclerotic process, many authors showed that collagen molecules are also subject to qualitative alterations.…”

“…[12] The newly synthesised collagen that constitutes the major ECM component, [28] marks the aggravation of the cellular alteration due to diabetes and causes fibrosis state. [29,30,31] In fact, the fibrosis of the aortic wall would be due to the presence of collagen, [32] which is dominant in the proliferative intima. [33] Our qualitative and quantitative study on the phenotypic expression of collagen has shown that cultured SMCs of the normal and diabetic Psammomys obesus synthesise more type I than type III collagen, in accord with the results of Layman et al [34] [36] have shown that SMC produced essentially collagen I and III.…”

The effect of low-dose fish oil supplementation on serum growth factors in healthy humans

“…Con-sequently, research has centred on the possibility of modulating these cells in order to reduce the development of atherosclerotic plaques. In addition to their role in primary lesion formation, smooth muscle cells and platelets are also, at least partly, responsible for the problem of restenosis following angioplasty (MacLeod et al, 1994;Markovitz et al, 1996) and its resultant clinical complications. A number of studies have reported that growth factors, released by vascular and circulatory cells at the site of the endothelial damage, mediate the proliferation of vascular smooth muscle cells.…”

Native LDL Induces Proliferation of Human Vascular Smooth Muscle Cells via Redox-Mediated Activation of ERK 1/2 Mitogen-Activated Protein Kinases

“…Human VSMC were freshly isolated from human umbilical cord veins (nϭ11) using explant technique 28 and cultured in Promocell® smooth muscle cell growth medium (Gibco) including 5% FCS. Cells were passaged by treatment with 0.05% trypsin/0.02% EDTA in PBS.…”