Proliferation and neurogenesis of neural stem cells enhanced by cerebral microvascular endothelial cells

Guo, Ying; Shi, De-jin; Li, Wensheng; Liang, Chaofeng; Wang, Hui; Ye, Zhuopeng; Hu, Liping; Wang, Huaqiao; Li, B M Yan

doi:10.1002/micr.20443

Cited by 15 publications

(13 citation statements)

References 15 publications

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“…The wells were viewed regularly under an inverted microscope (Olympus, Japan). Our observations confirmed that the CEC plated in the upper compartment were unable to migrate through the membrane pores into the space containing the BEPC, whereas the secreted cytokines were free to move [Guo et al, 2008]. After 10 days of coculture, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were also used to examine the shape and presence of tight junctions in the induced BEPC.…”

Section: Coculture Of Bepc and Cecsupporting

confidence: 56%

Bone Marrow-Derived Endothelial Progenitor Cells: A Promising Therapeutic Alternative for Corneal Endothelial Dysfunction

Shao

et al. 2010

Cells Tissues Organs

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Background: The global shortage of donor corneas has motivated the development of bioengineered corneas. Although corneal endothelium has been reconstituted using corneal endothelial cells (CEC) and precursor cells with various carrier materials, all of the current options require corneal tissue and are also limited by the scarcity of donor corneas. Here, we explored the feasibility of inducing bone marrow-derived endothelial progenitor cells (BEPC) to differentiate into CEC for the repair of corneal endothelial defects. Materials and Methods: BEPC were isolated from human fetal bone marrow, and identified using several antigen markers. BEPC were cocultured with CEC for 10 days in a transwell system with conditioned medium from CEC, and cell transdifferentiation was then examined. With a porcine corneal acellular matrix (PCACM) as the carrier, the induced BEPC were transplanted onto a cat’s cornea from which Descemet’s membrane and the endothelium had been stripped. Results: The induced BEPC resembled CEC in polygonal shape, expressing aquaporin-1, tightly opposed cell junctions, and neurone-specific enolase. Twenty-eight days after surgery, the transparency gradually returned to the corneas transplanted with the induced BEPC on PCACM. Conclusions: Human fetal BEPC transdifferentiate into corneal endothelial-like cells in vitro. Features of the induced BEPC indicated that they may be useful for the repair of corneal endothelial dysfunction.

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Section: Coculture Of Bepc and Cecsupporting

confidence: 56%

Bone Marrow-Derived Endothelial Progenitor Cells: A Promising Therapeutic Alternative for Corneal Endothelial Dysfunction

Shao

et al. 2010

Cells Tissues Organs

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“…1) An important mechanism seems to be the expression of neurotrophic factors [28]. 2) Progenitor cells are capable to enhance endogenous neurogenesis of neural stem cells in the CNS via angiogenesis [29][30][31]. 3) In addition, in vitro studies have demonstrated the ability of HSCs to transdifferentiate into neuron-like cells [12,32].…”

Section: Discussionmentioning

confidence: 99%

Increased Circulating Progenitor Cells in Alzheimer's Disease Patients with Moderate to Severe Dementia: Evidence for Vascular Repair and Tissue Regeneration?

Stellos

Panagiota

Sachsenmaier

et al. 2010

JAD

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Abstract. Cerebrovascular dysfunction is a common finding in patients with Alzheimer's disease (AD) and may contribute to cognitive decline. Abundant evidence suggests that vascular and neuronal repair mechanisms are mediated by circulating progenitor cells in vivo. Whether CD34 + and, specifically, CD34 + /CD133 + progenitor cells are involved in the pathophysiology of AD is poorly understood so far. In the present study, peripheral blood concentrations of circulating CD34 + /CD133 + and CD34+ progenitor cells were measured in 45 AD patients and in 30 healthy elderly controls by flow cytometry. The severity of dementia was assessed by Mini-Mental Status Examination and Clinical Dementia Rating scale. AD patients were stratified into two groups showing mild (n = 17) and moderate to severe (n = 28) dementia. In the present study, AD patients with moderate to severe dementia, but not those with mild dementia, showed significantly increased circulating CD34 + /CD133 + and CD34 + progenitor cells compared to healthy elderly controls independent of cardiovascular risk factors and medication. In addition, the number of circulating CD34 + /CD133 + progenitor cells in AD patients was significantly inversely correlated with cognitive function, age, and plasma levels of SDF-1, the most potent chemokine for progenitor cells. Our findings suggest a stage-dependent upregulation of circulating CD34 + /CD133 + and CD34 + progenitor cells in AD patients, which could take part in tissue healing processes of the brain in AD.

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“…Consequently, the majority of molecules shown to be essential for dentate gyrus development are most well known for neurogenesis that occurs during early brain development (Doetsch, 2003;Guo et al, 2008;Riquelme et al, 2008;Zhao et al, 2008). Molecules specific to neurogenesis within the more mature dentate gyrus remain elusive.…”

Section: Introductionmentioning

confidence: 99%

ApoE is required for maintenance of the dentate gyrus neural progenitor pool

et al. 2011

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SUMMARYMany genes regulating adult neurogenesis have been identified and are known to play similar roles during early neuronal development. We recently identified apolipoprotein E (ApoE) as a gene the expression of which is essentially absent in early brain progenitors but becomes markedly upregulated in adult dentate gyrus stem/progenitor cells. Here, we demonstrate that ApoE deficiency impairs adult dentate gyrus development by affecting the neural progenitor pool over time. We utilized ApoEdeficient mice crossed to a nestin-GFP reporter to demonstrate that dentate gyrus progenitor cells proliferate more rapidly at early ages, which is subsequently accompanied by an overall decrease in neural progenitor cell number at later time points. This appears to be secondary to over-proliferation early in life and ultimate depletion of the Type 1 nestin-and GFAP-expressing neural stem cells. We also rescue the proliferation phenotype with an ApoE-expressing retrovirus, demonstrating that ApoE works directly in this regard. These data provide novel insight into late hippocampal development and suggest a possible role for ApoE in neurodegenerative diseases.

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Proliferation and neurogenesis of neural stem cells enhanced by cerebral microvascular endothelial cells

Cited by 15 publications

References 15 publications

Bone Marrow-Derived Endothelial Progenitor Cells: A Promising Therapeutic Alternative for Corneal Endothelial Dysfunction

Bone Marrow-Derived Endothelial Progenitor Cells: A Promising Therapeutic Alternative for Corneal Endothelial Dysfunction

Increased Circulating Progenitor Cells in Alzheimer's Disease Patients with Moderate to Severe Dementia: Evidence for Vascular Repair and Tissue Regeneration?

ApoE is required for maintenance of the dentate gyrus neural progenitor pool

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