Proliferation and β-tubulin for human aortic endothelial cells within gas-plasma scaffolds

Bailey, Steven R.; Polan, Jodie; Munoz, Oscar C.; Agrawal, Mauli; Goswami, Nilesh

doi:10.1016/j.carrad.2004.08.001

Cited by 6 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Synthetic scaffold materials have included poly(lactide-co-caprolactone) [14], polylactide [33], polycaprolactone [34], poly(lactide-co-glycolide) [35], polydioxanone [36], poly(diol citrate) [5], poly(glycerol sebacate) [4] and polyhydroxyalkanoate [37]. These materials have been generated with numerous techniques including wet-spinning [38], salt leaching [39], thermally induced phase separation [6, 35], electrospinning [8, 14, 15, 34, 36] and combination approaches [5, 33, 40–42]. Synthetic polymers have also been blended with structural biomacromolecules collagen and elastin during graft processing [8, 15, 36, 43]…”

Section: Discussionmentioning

confidence: 99%

A small diameter, fibrous vascular conduit generated from a poly(ester urethane)urea and phospholipid polymer blend

et al. 2009

View full text Add to dashboard Cite

The thrombotic and hyperplastic limitations associated with synthetic small diameter vascular grafts has generated sustained interest in finding a tissue engineering solution for autologous vascular segment generation in situ. One approach is to place a biodegradable scaffold at the site that would provide acute mechanical support while vascular tissue develops. To generate a scaffold that possessed both non-thrombogenic character and mechanical properties appropriate for vascular tissue, a biodegradable poly(ester urethane)urea (PEUU) and non-thromobogenic bioinspired phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine-co-methacryloyloxyethyl butylurethane) (PMBU) were blended at PMBU weight fractions of 0–15% and electrospun to create fibrous scaffolds. The composite scaffolds were flexible with breaking strains exceeding 300%, tensile strengths of 7–10 MPa and compliances of 2.9–4.4 × 10−4 mmHg−1. In vitro platelet deposition on the scaffold surfaces significantly decreased with increasing PMBU content. Rat smooth muscle cell proliferation was also inhibited on PEUU/PMBU blended scaffolds with greater inhibition at higher PMBU content. Fibrous vascular conduits (1.3 mm inner diameter) implanted in the rat abdominal aorta for 8 weeks showed greater patency for grafts with 15% PMBU blending versus PEUU without PMBU (67% versus 40%). A thin neo-intimal layer with endothelial coverage and good anastomotic tissue integration was seen for the PEUU/PMBU vascular grafts. These results are encouraging for further evaluation of this technique in larger diameter applications for longer implant periods.

show abstract

Section: Discussionmentioning

confidence: 99%

A small diameter, fibrous vascular conduit generated from a poly(ester urethane)urea and phospholipid polymer blend

et al. 2009

View full text Add to dashboard Cite

show abstract

“…It requires cellular ingrowth from native tissues along with material degradation, then eventually forms a functional vascular replacement. The SDVGs prepared from biodegradable synthetic polymers, such as polylactide [3,4], poly(glycerol sebacate) [5], polycaprolactone [6,7] and polyurethane [8], have been investigated for this purpose. However, the implanted material would induce acute thrombosis and intimal hyperplasia (IH, smooth muscle cell over-proliferation), which are two major barriers to the biodegradable SDVGs.…”

Section: Introductionmentioning

confidence: 99%

Electrospun biodegradable elastic polyurethane scaffolds with dipyridamole release for small diameter vascular grafts

et al. 2014

View full text Add to dashboard Cite

Acellular biodegradable small diameter vascular grafts (SDVGs) require antithrombosis, intimal hyperplasia inhibition and rapid endothelialization to improve the graft patency. However, current antithrombosis and antiproliferation approaches often conflict with endothelial cell layer formation on SDVGs. To address this limitation, biodegradable elastic polyurethane urea (BPU) and the drug dipyridamole (DPA) were mixed and then electrospun into a biodegradable fibrous scaffold. The BPU would provide the appropriate mechanical support, while the DPA in the scaffold would offer biofunctions as required above. We found that the resulting scaffolds had tensile strengths and strains comparable with human coronary artery. The DPA in the scaffolds was continuously released up to 91 days in phosphate buffer solution at 37 °C, with a low burst release within the first 3 days. Compared to BPU alone, improved non-thrombogenicity of the DPA-loaded BPU scaffolds was evidenced with extended human blood clotting time, lower TAT complex concentration, lower hemolysis and reduced human platelet deposition. The scaffolds with a higher DPA content (5 and 10%) inhibited proliferation of human aortic smooth muscle cell significantly. Furthermore, the DPA-loaded scaffolds had no adverse effect on human aortic endothelial cell growth, yet it improved their proliferation. The attractive mechanical properties and biofunctions of the DPA-loaded BPU scaffold indicate its potential as an acellular biodegradable SDVG for vascular replacement.

show abstract

“…The measurement is based on the ability of mitochondrial dehydrogenases of viable cells to cleave tetrazolium salts (2). Two hours after cell plating, HUVECs were treated with PF14 (1 g/ml; Ϸ26 nmol/l) or solvent alone for 48 h. Cell proliferation reagent 4-[3-[4-iodophenyl]-2-4(4-nitrophenyl)]-2H-5-tetrazolio-1,3-benzene disulfonate (WST-1, 10 l/well) (Roche Diagnostics, Meylan, France) was added.…”

Section: Huvec Proliferationmentioning

confidence: 99%

Microfibrils and fibrillin-1 induce integrin-mediated signaling, proliferation and migration in human endothelial cells

Mariko

Ghandour

Raveaud

et al. 2010

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

show abstract

Proliferation and β-tubulin for human aortic endothelial cells within gas-plasma scaffolds

Cited by 6 publications

References 20 publications

A small diameter, fibrous vascular conduit generated from a poly(ester urethane)urea and phospholipid polymer blend

A small diameter, fibrous vascular conduit generated from a poly(ester urethane)urea and phospholipid polymer blend

Electrospun biodegradable elastic polyurethane scaffolds with dipyridamole release for small diameter vascular grafts

Microfibrils and fibrillin-1 induce integrin-mediated signaling, proliferation and migration in human endothelial cells

Contact Info

Product

Resources

About