Proliferation of anterior cruciate ligament cells in vitro by photo‐immobilized epidermal growth factor

Abstract

The purpose of this study was to explore the early treatment potential of anterior cruciate ligament (ACL) injuries using artificial juxtacrine stimulation by photo-immobilization of a growth factor. A photo-reactive epidermal growth factor complex (EGF-Az) was synthesized by conjugating EGF with N-(4-azidobenzoyloxy) succinimide followed by immobilization onto polystyrene culture plates using UV irradiation. ACL cells from human tissues (1 Â 10 5 cells, 100 ml/ well) were cultured as follows: control, no EGF; 50 ml native EGF; 50 ml EGF-Az immobilized; and 100 ml EGF-Az immobilized. The ACL cells were cultured long-term and evaluated for possible differences in their responses to EGF. An in vitro wound closure assay was developed to enable examination of cellular proliferation and migration. ACL cell proliferation was most evident in the photo-immobilized EGF culture group and was seen to increase in proportion to the amount of added EGF. In the in vitro wound closure assay, the lesioned area at 72 h after culture initiation was indistinguishable in the photo-immobilized cultures, but remained clearly visible in the controls. We conclude that photo-immobilized EGF induced rapid proliferation of ACL fibroblast cells by artificial juxtacrine stimulation and speculate that similar EGF immobilization onto bioabsorbable material (e.g., polyglycolic acid or polylactic acid) might contribute to a new therapy for the treatment of ACL injuries. ß

Understanding the Role of Growth Factors in Modulating Stem Cell Tenogenesis

“…EGF is a potent mitogen that participates in MSCs and fibroblast proliferation [12], [13], and is also involved in the initial phase of tendon healing. Besides MSCs proliferation, EGF treatment also preserves early progenitors within a MSC population [12], and increases the paracrine activity of stem cells.…”

Human adipose tissue‐derived tenomodulin positive subpopulation of stem cells: A promising source of tendon progenitor cells

“…As a first approach, subpopulations of hASCs positive for TNMD, STRO-1, CD29, and SSEA-4 markers were isolated and their tenogenic differentiation capacity assessed for 28 days upon culturing cells with various growth factors [GFs: basic-fibroblast GF (bFGF), transforming GF-β1 (TGF-β1) and platelet-derived GF (PDGF-BB)] that are well known regulators of the development and healing of tendon tissue (James, Kesturu, Balian, & Chhabra, 2008;Molloy, Wang, & Murrell, 2003;Woo, Kwon, Lee, & Park, 2007. ) and described to participate in stem cell tenogenesis (Goncalves et al, 2013;James et al, 2008;Nourissat, Berenbaum, & Duprez, 2015).…”

Positive effects of platelet-rich plasma (PRP) and a Sanguisorba officinalis polysaccharide on the proliferation and differentiation of anterior cruciate ligament (ACL) fibroblasts in vitro

“…By comparing with the retention time and peak areas of the standards under the same conditions, GC analysis showed SOWPa was composed of glucose and galactose in a molar ratio of 2.01:1.01. Both proliferation and migration of ACL fibroblasts are essential for repair and regeneration (Woo et al 2007). The results from current studies have demonstrated that PRP, SOWPa (100 mg/kg) or PRP þ SOWPa could significantly promote the proliferation and migration of ACL fibroblasts, especially at 72 h post seeding.…”