Proliferation of diversified clostridial species during biological soil disinfestation incorporated with plant biomass under various conditions

Abstract

・YOU Campus Repository System is built to collect, archive and offer electronically academic documents produced by University Consortium Yamagata. ・The copyright and related rights of the article are held by authors or publishers. ・You must require the permission of the copyright owner if you want to use over the curtailment of copyrights. ・Author-version manuscripts may have some differences in layouts and wordings from publisher version. were commonly recognized as closely related species of the dominant clone groups from these soil samples.

Suppression of spinach wilt disease by biological soil disinfestation incorporated with Brassica juncea plants in association with changes in soil bacterial communities

“…Spinach plants cultivated in all BSD-treated soils with B. juncea incorporation showed much lower disease incidences as compared with the non-treated or control plots for both experiments. The results were similar to our previous studies of model experiments (Mowlick et al, 2012(Mowlick et al, , 2013, indicating the effectiveness of BSD using B. juncea plants for actual cultivation of spinach in the field conditions.…”

“…Similar results were obtained from the analysis of bacterial community for the control soil (without biomass) of our model experiment (Mowlick et al, 2013).…”

“…1) contained diversified bacterial groups of different phyla and classes as noticed in case of non-treated soils of the previous studies (Mowlick et al, 2012(Mowlick et al, , 2013). Since we obtained almost similar community profiles for the non-treated and control (without biomass) soils in the previous studies as well as Exp.…”

“…pfennigii, and P. schinkii were also placed as the dominant clone groups for our model experiments (Mowlick et al, 2012(Mowlick et al, , 2013. These diverse clostridial species are known to form various products including VFAs, alcohols or other compounds such as indole or skatole during decomposition of biomass (Macfarlane and Macfarlane, 1995;Rainey et al, 2009;Wiegel, 2009), suggesting the possible role of pathogenic suppression by the fermentation products of the related species in BSD-treated soil in practice.…”

“…lycopersici, wilt pathogen of tomato and F. oxysporum f. sp. spinacea, wilt pathogen of spinach) in two pot experiments using soil from different districts of Japan (Mowlick et al, 2012(Mowlick et al, , 2013. Based on the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and clone library analyses, we found that bacterial communities in the BSD-treated soils were greatly changed and strictly anaerobic groups, especially fermentative members in the class Clostridia, became major bacterial groups in the communities together with some other aerobic or facultatively anaerobic bacteria from the classes including Bacilli and Gammaproteobacteria.…”