Proliferation of fibroblasts cultured on a hemi-cellulose dressing

Ferreira, Lydia Masako; Sobral, Christiane Steponavicius; Blanes, Leila; Ipolito, Michelle Zampieri; Horibe, Elaine K.

doi:10.1016/j.bjps.2009.01.086

Cited by 20 publications

(12 citation statements)

References 30 publications

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“…In fact, proliferation decreases with time of culture. This is contradictory to what has been reported previously (Ferreira et al, 2010; Andersson et al, 2010; Saska et al, 2012). This difference could be due to the experimental condition used including seeding concentration.…”

Section: Discussioncontrasting

confidence: 84%

Hydroxyapatite bioactivated bacterial cellulose promotes osteoblast growth and the formation of bone nodules

et al. 2012

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The goal of this study was to investigate the feasibility of bacterial cellulose (BC) scaffold to support osteoblast growth and bone formation. BC was produced by culturing Acetobacter xylinum supplemented with hydroxyapatite (HA) to form BC membranes (without HA) and BC/HA membranes. Membranes were subjected to X-ray photoelectron spectroscopy (XPS) analysis to determine surface element composition. The membranes were further used to evaluate osteoblast growth, alkaline phosphatase activity and bone nodule formation. BC was free of calcium and phosphate. However, XPS analysis revealed the presence of both calcium (10%) and phosphate (10%) at the surface of the BC/HA membrane. Osteoblast culture showed that BC alone was non-toxic and could sustain osteoblast adhesion. Furthermore, osteoblast adhesion and growth were significantly (p ≤0.05) increased on BC/HA membranes as compared to BC alone. Both BC and BC/HA membranes improved osteoconductivity, as confirmed by the level of alkaline phosphatase (ALP) activity that increased from 2.5 mM with BC alone to 5.3 mM with BC/HA. BC/HA membranes also showed greater nodule formation and mineralization than the BC membrane alone. This was confirmed by Alizarin red staining (ARS) and energy dispersive X-ray spectroscopy (EDX). This work demonstrates that both BC and BC/HA may be useful in bone tissue engineering.

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Section: Discussioncontrasting

confidence: 84%

Hydroxyapatite bioactivated bacterial cellulose promotes osteoblast growth and the formation of bone nodules

et al. 2012

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“…Furthermore, cellulosic tissue engineering scaffolds derived from decellularized apple slices have shown the ability for mammalian cell attachment and proliferation [22] and were found to be biocompatible when implanted subcutaneously in vivo [23]. Pectin [24] and hemicellulose [25] have also been studied as biomaterials for bone tissue engineering and wound healing, respectively. The innate similarities and apparent biocompatibility of plant ECM spurred us to look across kingdoms and investigate whether plants and their innate vasculatures could serve as perfusable scaffolds for engineering human tissue.…”

Section: Introductionmentioning

confidence: 99%

Crossing kingdoms: Using decellularized plants as perfusable tissue engineering scaffolds

et al. 2017

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Despite significant advances in the fabrication of bioengineered scaffolds for tissue engineering, delivery of nutrients in complex engineered human tissues remains a challenge. By taking advantage of the similarities in the vascular structure of plant and animal tissues, we developed decellularized plant tissue as a prevascularized scaffold for tissue engineering applications. Perfusion-based decellularization was modified for different plant species, providing different geometries of scaffolding. After decellularization, plant scaffolds remained patent and able to transport microparticles. Plant scaffolds were recellularized with human endothelial cells that colonized the inner surfaces of plant vasculature. Human mesenchymal stem cells and human pluripotent stem cell derived cardiomyocytes adhered to the outer surfaces of plant scaffolds. Cardiomyocytes demonstrated contractile function and calcium handling capabilities over the course of 21 days. These data demonstrate the potential of decellularized plants as scaffolds for tissue engineering, which could ultimately provide a cost-efficient, “green” technology for regenerating large volume vascularized tissue mass.

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“…It is synthesized by various bacteria and has already proved to present great potential for wound healing applications [147,148]. Its high mechanical strength, crystallinity and high capacity to retain water mostly arise from its unique nanofibrillar structure [143,147].…”

Section: Cellulose and Its Derivativesmentioning

confidence: 99%

Recent advances on the development of wound dressings for diabetic foot ulcer treatment—A review

et al. 2013

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a b s t r a c tDiabetic foot ulcers (DFUs) are a chronic, non-healing complication of diabetes that lead to high hospital costs and, in extreme cases, to amputation. Diabetic neuropathy, peripheral vascular disease, abnormal cellular and cytokine/chemokine activity are among the main factors that hinder diabetic wound repair. DFUs represent a current and important challenge in the development of novel and efficient wound dressings. In general, an ideal wound dressing should provide a moist wound environment, offer protection from secondary infections, remove wound exudate and promote tissue regeneration. However, no existing dressing fulfills all the requirements associated with DFU treatment and the choice of the correct dressing depends on the wound type and stage, injury extension, patient condition and the tissues involved. Currently, there are different types of commercially available wound dressings that can be used for DFU treatment which differ on their application modes, materials, shape and on the methods employed for production. Dressing materials can include natural, modified and synthetic polymers, as well as their mixtures or combinations, processed in the form of films, foams, hydrocolloids and hydrogels. Moreover, wound dressings may be employed as medicated systems, through the delivery of healing enhancers and therapeutic substances (drugs, growth factors, peptides, stem cells and/or other bioactive substances). This work reviews the state of the art and the most recent advances in the development of wound dressings for DFU treatment. Special emphasis is given to systems employing new polymeric biomaterials, and to the latest and innovative therapeutic strategies and delivery approaches.

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Proliferation of fibroblasts cultured on a hemi-cellulose dressing

Cited by 20 publications

References 30 publications

Hydroxyapatite bioactivated bacterial cellulose promotes osteoblast growth and the formation of bone nodules

Hydroxyapatite bioactivated bacterial cellulose promotes osteoblast growth and the formation of bone nodules

Crossing kingdoms: Using decellularized plants as perfusable tissue engineering scaffolds

Recent advances on the development of wound dressings for diabetic foot ulcer treatment—A review

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