Proliferationsrate beim kleinzelligen Bronchialkarzinom

Kayser, Gian; Gunzenhäuser, I.; Becker, H. D.; Herth, Fjf; Kayser, K

doi:10.1007/s002920100480

Cited by 7 publications

(3 citation statements)

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“…There are indications, however, that a Ki-67 protein expression is an absolute requirement for progression through the cell-division cycle ( 55 ). Some studies ( 56 – 58 ) did not find a correlation with response after chemoradiation, similar to our results, but other studies show a correlation of the proliferation rate with the prognosis ( 59 , 60 ).…”

Section: Discussionsupporting

confidence: 90%

Immunohistochemistry and Radiomic Features for Survival Prediction in Small Cell Lung Cancer

et al. 2020

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Background: The aim of the study was to evaluate the role of different immunohistochemical and radiomics features in patients with small cell lung cancer (SCLC). Methods: Consecutive patients with histologically proven SCLC with limited (n = 47, 48%) or extensive disease (n = 51, 52%) treated with radiotherapy and chemotherapy at our department were included in the analysis. The expression of different immunohistochemical markers from the initial tissue biopsy, such as CD56, CD44, chromogranin A, synaptophysin, TTF-1, GLUT-1, Hif-1 a, PD-1, and PD-L1, and MIB-1/KI-67 as well as LDH und NSE from the initial blood sample were evaluated. H-scores were additionally generated for CD44, Hif-1a, and GLUT-1. A total of 72 computer tomography (CT) radiomics texture features from a homogenous subgroup (n = 31) of patients were correlated with the immunohistochemistry, the survival (OS), and the progression-free survival (PFS). Results: The median OS, calculated from diagnosis, was 21 months for patients with limited disease and 13 months for patients with extensive disease. The expression of synaptophysin correlated with a better OS (HR 0.546 95% CI 0.308-0.966, p = 0.03). The expression of TTF-1 (HR 0.286, 95% CI: 0.117-0.698, p = 0.006) and a lower GLUT-1 H-score (median = 50, HR: 0.511, 95% CI: 0.260-1.003, p = 0.05) correlated with a better PFS. Patients without chromogranin A expression had a higher risk for developing cerebral metastases (p = 0.02) and patients with PD 1 expression were at risk for developing metastases (p = 0.02). Our radiomics analysis did not reveal a single texture feature that correlated highly with OS or PFS. Correlation coefficients ranged between −0.48 and 0.39 for OS and between −0.46 and 0.38 for PFS. Gkika et al. Immunohistochemistry and Radiomics in SCLC Conclusions: The role of synaptophysin should be further evaluated as synaptophysin-negative patients might profit from treatment intensification. We report an, at most, moderate correlation of radiomics features with overall and progression free survival and no correlation with the expression of different immunohistochemical markers.

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Section: Discussionsupporting

confidence: 90%

Immunohistochemistry and Radiomic Features for Survival Prediction in Small Cell Lung Cancer

et al. 2020

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“…In contrast, only two studies to date have evaluated the association between SCLC and the Ki-67 proliferation index, one of which reported that the outcome was poor in patients with biopsy samples yielding a Ki-67 proliferation index lower than the median [12]. Another study has reported that there was no association between survival and the Ki-67 proliferation index in SCLC [13].…”

Section: Discussionmentioning

confidence: 99%

Correlation between the Ki-67 proliferation index and response to radiation therapy in small cell lung cancer

et al. 2017

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BackgroundIn the breast cancer, the decision whether to administer adjuvant therapy is increasingly influenced by the Ki-67 proliferation index. In the present retrospective study, we investigated if this index could predict the therapeutic response to radiation therapy in small cell lung cancer (SCLC).MethodsData from 19 SCLC patients who received thoracic radiation therapy were included. Clinical staging was assessed using the TNM classification system (UICC, 2009; cstage IIA/IIB/IIIA/IIIB = 3/1/7/8). Ki-67 was detected using immunostained tumour sections and the Ki-67 proliferation index was determined using e-Count software. Radiation therapy was administered at total doses of 45–60 Gy. A total of 16 of the 19 patients received chemotherapy.ResultsPatients were divided into two groups, one with a Ki-67 proliferation index ≥79.77% (group 1, 8 cases) and the other with a Ki-67 proliferation index <79.77% (group 2, 11 cases). Following radiation therapy, a complete response (CR) was observed in six cases from group 1 (75.0%) and three cases from group 2 (27.3%). The Ki-67 proliferation index was significantly correlated with the CR rate (P = 0.05), which was significantly higher in group 1 than in group 2 (P = 0.04). The median survival time was 516 days for all patients, and the survival rates did not differ significantly between groups 1 and 2.ConclusionsOur study is the first to evaluate the correlation between the Ki-67 proliferation index and SCLC tumour response to radiation therapy. Our findings suggest that a high Ki-67 proliferation index might represent a predictive factor for increased tumour radiosensitivity.

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“…Plastination was invented in 1946 by Romaniak, as a technique of tissue preservation in which water and lipids from biological specimens were gradually replaced by curable polymers that then harden, leading to hard, dry, odorless display specimens that should ideally retain the original shape and color of the respective organs [14]. Romaniak utilized unpolymerized resin, and later on, Von Hagens and others used polymerizing resins to gradually replace intermediary solvents such as acetone and xylene [1,2,[15][16][17][18]. Different types of resins have been reported with different results regarding the color and volume of the final display specimens, and the main advantage of the plastination technique is that it allows for the preservation of the complete anatomical topography, and thus the morphological characteristics of the anatomical structures.…”

Section: Discussionmentioning

confidence: 99%

Simple Universal Whole-Organ Resin-Embedding Protocol for Display of Anatomical Structures

et al. 2023

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Whole-organ plastic resin casting is a very useful method for preserving rare pathological specimens for forensic/anatomical studies and for teaching/research purposes. Many techniques have been proposed over time, but most of them use special non-commercially available resin mixtures, lengthy protocols, and are overall not easily implemented in any anatomy/pathology department that might need such a procedure for rapid organ preservation. Here, we utilized anatomical sections of the human brain, heart, kidneys, spleen, large intestine, and lungs from on-display organs that were fixed for more than 1 year in 10% neutral-buffered formalin and from a freshly processed cadaver for teaching purposes in our Human Anatomy Department, and we optimized a fast-processing protocol without the use of any clearing agents, which yields solid, clear, cylindrical resin casting blocks. The resulting protocol, which takes no longer than 4 days, proves that at least three commonly used epoxy resins from hobby shops can be utilized without any restrictions, and the use of resin or glycerin vacuum-forced impregnation even offers two choices of intrinsic contrast, depending on the nature of the preparation. A number of innovations have been included here and compared to existing publications, such as the use of a system of permanent fixation plexiglas rods that maintain the organ in the desired position and become invisible in the final block, the use of UVC sterilization of the tissue to ensure a long shelf life of the block, and the utilization of cheap cylindrical polypropylene food containers as casting molds. Altogether, we present a simple resin-embedding protocol that can be made available to any department/institution without the need for expensive materials and specially trained personnel.

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Proliferationsrate beim kleinzelligen Bronchialkarzinom

Abstract: Immune histochemical determination of the Ki-67 protein is a useful method to estimate the prognosis of patients with small cell lung carcinoma.

Cited by 7 publications

References 0 publications

Immunohistochemistry and Radiomic Features for Survival Prediction in Small Cell Lung Cancer

Immunohistochemistry and Radiomic Features for Survival Prediction in Small Cell Lung Cancer

Correlation between the Ki-67 proliferation index and response to radiation therapy in small cell lung cancer

Simple Universal Whole-Organ Resin-Embedding Protocol for Display of Anatomical Structures

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