Proliferative activities of epithelial and connective tissue cells in the rat periodontal regeneration using argyrophilic nucleolar organizer regions staining

Usuda, Jun; Hashimoto, Sadamitsu; Enokiya, Yasunobu; Inoue, Takashi; Shimono, Masaki

doi:10.1111/j.1600-0765.2004.00721.x

Cited by 7 publications

(2 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All study groups showed a degree of epithelial downgrowth when considering normal structure. This is known to be a common finding in periodontium being regenerated after loss (Usuda et al, 2004). However, our results showed more favorable results with P 11 -4 as shown by the significant decrease in epithelial downgrowth after 4 weeks in the P 11 -4 treated group.…”

Section: Discussioncontrasting

confidence: 44%

An in-vivo Intraoral Defect Model for Assessing the Use of P11-4 Self-Assembling Peptide in Periodontal Regeneration

El-Sayed

Davies

El-Zehery

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Periodontal disease is one of the most common diseases worldwide. It has a significant impact on oral health and subsequently the individual's quality of life. However, optimal regeneration of periodontal tissues, using current treatments, has yet to be achieved. Peptide self-assembly has provided a step-change in nanobiotechnology and regenerative medicine fields. Our aim was to investigate the effects of a selfassembling peptide (SAP; P 11-4) on periodontal regeneration in a preclinical model. Twenty-six bilateral maxillary critical-sized periodontal defects were created surgically in 13 rats. Defects on one side of the mouth were filled with P 11-4 hydrogel; the contralateral defect was untreated (control). Rats were sacrificed immediately post-surgery (time 0) and after 2 and 4 weeks. Retrieved maxillae were processed for histological, immunohistochemical, and histomorphometric assessments. The results of histological analysis showed greater organization of periodontal fibers in defects treated with P 11-4, at both time points, when compared to untreated defects. Histomorphometry showed that treated defects had both a significant increase in functional periodontal ligament length and a reduction in epithelial down growth after 4 weeks. At 2 weeks, treated defects showed a significant increase in expression of osteocalcin and osteoprotegerin as judged by immunohistochemistry. Also, a significantly higher osteoprotegerin/RANKL ratio was shown in treated defects. In conclusion, the results demonstrated enhanced regeneration of periodontal tissues when SAP P 11-4 was used to fill periodontal defects in rats. The findings of this study suggest that SAP P 11-4 is a promising novel candidate for periodontal regenerative therapy. Further investigations are required for optimization before clinical use.

show abstract

Section: Discussioncontrasting

confidence: 44%

An in-vivo Intraoral Defect Model for Assessing the Use of P11-4 Self-Assembling Peptide in Periodontal Regeneration

El-Sayed

Davies

El-Zehery

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…However, recent investigations using autoradiography demonstrated that a significant number of DAT cells are capable of synthesizing DNA, as do basal cells along the connective tissue (9, 10). The results of our previous studies using argyrophilic nucleolar organizer region (AgNORs) staining also support the idea that DAT cells have proliferative activity (20, 21).…”

Section: Discussionsupporting

confidence: 78%

Cytoskeleton and surface structures of cells directly attached to the tooth in the rat junctional epithelium

Ishikawa¹,

Hashimoto

Tanno

et al. 2005

J of Periodontal Research

View full text Add to dashboard Cite

show abstract

Comparison of the proliferative activity in gingival epithelium after surgical treatments of intrabony defects with bioactive glass and bioabsorbable membrane

et al. 2006

View full text Add to dashboard Cite

Guided tissue regeneration is based on preventing the more rapidly proliferating epithelium from growing into the periodontal defect after surgical procedures incorporating barrier membranes. The aim of this study was to compare the proliferative activity of gingival epithelium using proliferating cell nuclear antigen (PCNA) as a marker of cell proliferation after surgical treatments with bioactive glass graft material and bioabsorbable membrane. Using split mouth design, 20 intrabony defects were randomly assigned treatments with bioactive glass (BG group) or bioabsorbable membrane (BM group). Gingival biopsies were taken at preoperative and postoperative 12 weeks. After histological processing, the number of the inflammatory cells was measured in hematoxylin and eosin-stained sections; PCNA expression was determined in immunohistochemically-stained sections. At postoperative 12 weeks, the number of the inflammatory cells was significantly decreased (p < 0.01), PCNA expression was significantly increased (p < 0.001) in both treatment groups compared to baseline data. There was no significant difference in PCNA expression between baseline values of two groups (p > 0.05), while at postoperative 12 weeks, increase in BG group was significantly greater than that in BM group (p < 0.001). These results suggest that epithelial cell proliferation is more prominent after treatment of intrabony defects with bioactive glass compared to the treatment with bioabsorbable membrane.

show abstract

Proliferative activities of epithelial and connective tissue cells in the rat periodontal regeneration using argyrophilic nucleolar organizer regions staining

Cited by 7 publications

References 37 publications

An in-vivo Intraoral Defect Model for Assessing the Use of P11-4 Self-Assembling Peptide in Periodontal Regeneration

An in-vivo Intraoral Defect Model for Assessing the Use of P11-4 Self-Assembling Peptide in Periodontal Regeneration

Cytoskeleton and surface structures of cells directly attached to the tooth in the rat junctional epithelium

Comparison of the proliferative activity in gingival epithelium after surgical treatments of intrabony defects with bioactive glass and bioabsorbable membrane

Contact Info

Product

Resources

About