Proliferative activity in human breast cancer: Ki-67 automated evaluation and the influence of different Ki-67 equivalent antibodies

Fasanella, Silvia; Leonardi, Elena; Cantaloni, Chiara; Eccher, Claudio; Bazzanella, I; Aldovini, D; Bragantini, Emma; Morelli, Luca; Cuorvo, LV; Ferro, Antonella; Gasperetti, Fabio; Berlanda, G; Palma, Paolo Dalla; Barbareschi, Mattia

doi:10.1186/1746-1596-6-s1-s7

Cited by 88 publications

(74 citation statements)

References 17 publications

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“…The spontaneous intraductal papilloma from the tree shrew showed a very high percentage of Ki-67 positive cells, suggesting that the tumor cells were highly proliferative. The ratio of positive Ki-67 cells was very close to that of the human Ki-67 labeling index in breast cancers (Fasanella et al, 2011). In contrast, apoptosis is the most effective approach to eliminate transformed cells.…”

Section: Discussionmentioning

confidence: 75%

Characterization of spontaneous breast tumor in tree shrews (<I>Tupaia belangeri chinenesis</I>)

Xia¹,

Wang²,

Zhang³

et al. 2013

Zoological Research

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Breast cancer is a common malignant tumor. It is essential to develop suitable animal models for discovering novel preventive and therapeutic approaches. Tree shrews (Tupaia belangeri chinensis) have a closer evolutionary relationship with humans than do rodents, which have been widely used in laboratory research. Spontaneous breast tumors were identified in tree shrews in 1960s; however, no detailed studies about tree shrew breast tumors have been conducted to date. Here, we characterized a spontaneous breast tumor from tree shrews by Haematoxylin Eosin (H&E) staining. This tumor was identified as a papillary tumor. Immunohistochemical staining (IHC) for progesterone receptor (PR), Ki-67 and cleaved caspase-3 showed that tumor cells were positive for PR, highly proliferative, and less apoptotic compared to normal breast epithelial cells. Thus, the spontaneous tumor of tree shrew is very close to human papillary tumors in terms of morphology and pathology and we concluded that tree shrew may be a suitable animal model for breast cancer research.

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Section: Discussionmentioning

confidence: 75%

Characterization of spontaneous breast tumor in tree shrews (<I>Tupaia belangeri chinenesis</I>)

Xia¹,

Wang²,

Zhang³

et al. 2013

Zoological Research

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“…Although some pathologists favor averaging the Ki-67 score over the entire sampled specimen, 30,47 others have suggested the selected region with the highest level (the hotspots) of Ki-67 should be selected for scoring. 49,50 The current recommendation by the International Ki-67 in Breast Cancer Working Group for determining Ki-67 expression is to count at least 500 invasive cancer cells and to include areas of high Ki-67 expression.…”

Section: Discussionmentioning

confidence: 99%

“…Automated scoring of Ki-67 by other methods has previously reported similar results to visual assessment and has been shown to minimize interobserver variability. 46,47 Analysis of Ki-67 by AQUA also eliminates the necessity of determining a cut point for percent positive nuclei. No consensus has been achieved regarding the ideal cut point between high and low Ki-67 staining in breast cancer, although levels between 10% and 20% are most common.…”

Section: Discussionmentioning

confidence: 99%

Quantitative assessment Ki-67 score for prediction of response to neoadjuvant chemotherapy in breast cancer

Brown

DiGiovanna

Killelea

et al. 2014

Laboratory Investigation

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Measurement of Ki-67, a marker of cell proliferation, has been associated with response to therapy, but methods of measurement are controversial. Here we use a quantitative objective measurement for Ki-67 to determine the best method for assessment of Ki-67 for prediction of response to neoadjuvant chemotherapy. Analysis was conducted on a cohort of 105 consecutive invasive breast cancer patients that received neoadjuvant therapy between 2002 and 2010, and on whom pre-surgical biopsies were obtainable. Ki-67 expression was measured using quantitative immunofluorescence automated quantitative analysis (AQUA) technology. Images for each specimen were collected for 5 to 115 fields of view (FOVs) and summary scores were obtained, corresponding to the average and maximum of all the FOVs. AQUA scoring (using both intensity and area) was comparable to automated calculation of percentage of positive nuclei for prediction of response to chemotherapy (OR: 2.832 vs 2.712). Both the average and maximum AQUA score showed Ki-67 expression was directly correlated to pathological complete response (pCR; average P ¼ 0.0002; maximum P ¼ 0.0011). Although examining the maximum FOV was more predictive of response to therapy (OR: 3.546 vs 2.832), averaging all fields provided more sensitivity and specificity (AUC 0.769 vs 0.732). Ki-67 average (P ¼ 0.0025) and maximum (P ¼ 0.0239) AQUA score were also significant predictors of pCR in a multivariable analysis, including tumor size, nuclear grade, nodal status, ER status, and HER2 status. Measurement of Ki-67 expression by objective quantitative methods shows increased Ki-67 levels are an independent predictor of response to neoadjuvant chemotherapy.

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“…With the application of computer automatic image analysis technology in the detection of prognostic markers in breast cancer [8][9][10][11][12], the counting method of nuclear fission image now becomes more and more accurately. In this study, we watched the relationship between number of cell mitoses and axillary lymph node metastasis.…”

Section: Discussionmentioning

confidence: 99%

Clinical pathological analysis in 104 ductal breast cancer cases

Jin¹,

Dai²

2017

Clin Diagn Pathol

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Ductal breast cancer is a clinically heterogeneous disease; we investigate the correlation between tumor size, comedo necrosis, number of cell mitoses and axillary lymph node metastasis in ductal breast cancer. In this study, 104 ductal breast cancer specimens were collected and divided into 3 groups T1 group (tumor size ≦ 2 cm), T2 group (2< tumor size≦5cm) and T3 group (tumor size＞5.0cm). Among those specimens, 95 cases were diagnosed with invasive ducted carcinoma, 9 cases were ductal carcinoma in-situ (DCIS). Results shown that T3 group has a higher rate of axillary lymph node metastasis than T2 group and T1 group; T2 group has a higher rate of lymph node metastasis than T1 group. The patients with the number of cell mitoses(≧10)were also has a higher rate of axillary lymph node metastasis (P = 0.0139) than the patients with the number of cell mitoses (<10). No significance was found between comedo necrosis lesions and axillary lymph node metastasis, though the frequency of comedo necrosis lesions in patients with axillary lymph node metastasis was higher than those in non-metastatic patients. It is concluded that the tumor size and the number of cell mitoses were risk factors for axillary lymph node involvement in ductal breast cancer.

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Proliferative activity in human breast cancer: Ki-67 automated evaluation and the influence of different Ki-67 equivalent antibodies

Cited by 88 publications

References 17 publications

Characterization of spontaneous breast tumor in tree shrews (<I>Tupaia belangeri chinenesis</I>)

Characterization of spontaneous breast tumor in tree shrews (<I>Tupaia belangeri chinenesis</I>)

Quantitative assessment Ki-67 score for prediction of response to neoadjuvant chemotherapy in breast cancer

Clinical pathological analysis in 104 ductal breast cancer cases

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