Proliferative activity of cells from remaining dental pulp in response to treatment with dental materials

Abstract

Background: The biological examination of pulp injury, repair events and response of dental pulp stem cells to dental restorative materials is important to accomplish restorative treatment, especially to commonly used dental materials in paediatric dentistry, such as glass ionomer cement (GIC) and calcium hydroxide (Ca(OH) 2 ) lining cement. Methods: Healthy patients aged between 9 to 11 years with carious primary molars without pulp exposure were selected and divided into two groups: Group 1 (teeth restored with GIC) and Group 2 (teeth lined using Ca(OH) 2 and restored with GIC). The proliferative activity of stem cells of teeth between these two groups was compared using colourimetric cell proliferation reagent, alamarBlue. Immunocytochemistry and flow cytometry confirmation were performed using mesenchymal stem cell markers, CD105 and CD166. Results: The proliferative activity using alamarBlue TM assay showed that cells derived from the remaining dental pulp of exfoliated deciduous teeth were positive for CD105 and CD166 and exhibited no difference between the two groups. Conclusions: It can be concluded that the use of Ca(OH) 2 or GIC as a lining material in indirect pulp capping procedures has the same effect on cells derived from the remaining dental pulp of exfoliated deciduous teeth which have responded favourably to the restorative treatments.

Cytotoxicity and bioactivity of various pulpotomy materials on stem cells from human exfoliated primary teeth

“…Moreover, the information obtained is both qualitative and quantitative (Lutfi et al . ). With Biodentine and MTA Angelus, the MSC surface molecules, CD73, CD90 and CD105, were expressed at levels >95%, whereas the expression of the haematopoietic markers, CD34 and CD45, was <5%.…”

“…In very deep cavities with remaining dentine thickness between 0.25 and 0.01 mm or in pulp exposure cavities, dental pulp stem cells proliferate, migrate to the site of injury and differentiate to form odontoblastoid cells (Lutfi et al . ). Cell migration is necessary for homoeostatic tissue maintenance and the regeneration of injured organs and tissues (Rennert et al .…”

“…), with adequate biological responses of SHEDs (Lutfi et al . ). On the other hand, eugenol in combination with zinc oxide (ZOE) is used in Paediatric Dentistry as a root canal sealer in pulpectomies and as a pulp sedative in cases of vital pulps.…”

Effect of biomaterials on angiogenesis during vital pulp therapy

“…Lutfi et al showed that glass ionomer cement (GIC) as a lining material can act similar to Ca(OH) 2 in inducing proliferative activity in dental pulp of exfoliated deciduous teeth 51) . Dammaschke et al compared the effect of dentine adhesive Gluma Comfort Bond (GCB) and Ca(OH) 2 on the proliferation of pulp cells and concluded that GCB, due to its missing antibacterial efficacy and foreign body reactions, causes the inflammation of pulp tissue.…”

Effects of mineral trioxide aggregate, calcium hydroxide, biodentine and Emdogain on osteogenesis, Odontogenesis, angiogenesis and cell viability of dental pulp stem cells

“…It is important to study the properties of different capping materials in the context of cell viability of regenerating cells, transcriptional profile and their ability to affect differentiation process. Calcium Hydroxide [Ca(OH) 2 ] is commonly used for direct pulp capping with sufficient biological responses [37]. However, the main disadvantages of Ca(OH) 2 are weak cohesive strength, marginal leakage and inadequate antibacterial effect [2].…”