Proliferative and Migratory Potentials of Human Cord Blood-Derived CD34 - Severe Combined Immunodeficiency Repopulating Cells That Retain Secondary Reconstituting Capacity

Kimura, Takafumi; Wang, Jianfeng; Matsui, Kazuo; Imai, Shiro; Yokoyama, Shigeki; Nishikawa, Mitsuo; Ikehara, Susumu; Sonoda, Yoshiaki

doi:10.1532/ijh97.03158

Cited by 18 publications

(67 citation statements)

References 28 publications

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“…The CB-derived lineage-negative (Lin À ) mononuclear cells were separated using a StemSep device (StemCell Technologies, Vancouver, BC, Canada), as reported earlier. [7][8][9] Purification of Lin À CD34 þ CD38 þ , Lin À CD34 þ CD38 À , and Lin À CD34 À cells…”

Section: Methodsmentioning

confidence: 99%

“…[7][8][9]13 In this study, purified 2.5 Â 10 3 to 2 Â 10 4 CB-derived Lin À CD34 þ CD38 þ , 20-4 Â 10 2 CBderived Lin À CD34 À CD38 À cells, or 2-7 Â 10 4 CB-derived Lin À CD34 À cells were transplanted by IBMI 7-9 into sublethally irradiated (250 cGy using a 137 Cs-g irradiator) 8-to 10-week-old NOD/SCID or NOG mice. The repopulation of human hematopoietic cells in murine BMs was serially analyzed by the aspiration method.…”

Section: Nod/scid and Nog Micementioning

confidence: 99%

“…In LT human hematopoietic cell reconstitution experiments using NOG mice, BMs were serially aspirated from the contralateral sites (right tibia) of IBMI of each mouse. In some of these experiments, the mice were killed 5,8,12, and 24 weeks after transplantation and the BMs from the pairs of femurs and tibiae of each mouse were flushed into a-medium. The repopulation of human hematopoietic cells in murine BMs was determined by detecting the number of cells positively stained with PC5-conjugated anti-human CD45 mAb (Coulter) by flow cytometry (FACS Calibur, Becton Dickinson).…”

Section: Nod/scid and Nog Micementioning

confidence: 99%

“…7 Functional studies revealed that these CD34 À SRCs have different HSC characteristics from earlier-reported CD34 þ SRCs. [7][8][9] These data suggested that the CD34 À SRCs are a novel class of primitive repopulating HSCs that can only be detected by the sensitive IBMI method. [7][8][9][10] On the other hand, Hogan et al 11 clearly showed that CB-derived SRCs present in the CD34 þ cell fraction could be segregated into two subpopulations with distinct repopulation characteristics.…”

Section: Introductionmentioning

confidence: 97%

“…[7][8][9] These data suggested that the CD34 À SRCs are a novel class of primitive repopulating HSCs that can only be detected by the sensitive IBMI method. [7][8][9][10] On the other hand, Hogan et al 11 clearly showed that CB-derived SRCs present in the CD34 þ cell fraction could be segregated into two subpopulations with distinct repopulation characteristics. The CD34 þ CD38 þ SRCs could rapidly repopulate the recipient NOD/SCID mice, but they could only maintain a human cell repopulation for 12 weeks, while showing no secondary repopulating potential.…”

Section: Introductionmentioning

confidence: 97%

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In vivo dynamics of human cord blood-derived CD34− SCID-repopulating cells using intra-bone marrow injection

et al. 2009

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The identification of human CD34-negative (CD34 À ) hematopoietic stem cells (HSCs) provides a new concept for the hierarchy in the human HSC compartment. This study investigated the long-term repopulating capacity and redistribution kinetics of human cord blood-derived CD34 À severe combined immunodeficiency (SCID)-repopulating cells (SRCs) and compared them with those of CD34 þ CD38 þ and CD34 þ CD38 À SRCs using the intra-bone marrow injection (IBMI) to clarify the characteristics of CD34 À SRCs. On the basis of the limiting dilution analyses data, estimated numbers of CD34 þ CD38 þ , CD34 þ CD38 À , and CD34 À SRCs were transplanted to NOD/SCID mice by IBMI. The human cell repopulation at the site of injection and the other bones were serially investigated. Interestingly, CD34 þ CD38 þ , CD34 þ CD38 À , and CD34 À SRCs began to migrate to other bones 2 and 5 weeks after the transplantation, respectively. Accordingly, the initiation of migration seemed to differ between the CD34 þ and CD34 À SRCs. In addition, CD34 þ CD38 þ SRCs only sustained a shortterm repopulation. However, both CD34 þ CD38 À and CD34 À SRCs had longer-term repopulation capacity. Taken together, these findings showed that CD34 À SRCs show different in vivo kinetics, thus suggesting that the identified CD34 À SRCs are a distinct class of primitive HSCs in comparison to the CD34 þ CD38 þ and CD34 þ CD38 À SRCs.

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Section: Methodsmentioning

confidence: 99%

Section: Nod/scid and Nog Micementioning

confidence: 99%

Section: Nod/scid and Nog Micementioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

See 3 more Smart Citations

In vivo dynamics of human cord blood-derived CD34− SCID-repopulating cells using intra-bone marrow injection

et al. 2009

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Human CD34-negative Hematopoietic Stem Cells

Sonoda

2014

Stem Cell Biology and Regenerative Medicine

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CD34-negative hematopoietic stem cells show distinct expression profiles of homing molecules that limit engraftment in mice and sheep

et al. 2017

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We and others have reported that human hematopoietic stem cells (HSCs) are also present in the CD34-negative (CD34) fraction of human cord blood (CB). Here, we examined the hematopoietic engraftment potential of 13 or 18 lineage-negative (13Lin or 18Lin) CD34 cells from human CB in mice and sheep. Both 13Lin and 18Lin CD34 cells efficiently engrafted in mice irrespective of transplantation route, be it by tail-vein injection (TVI) or by intra-bone marrow injection (IBMI). These cells also engrafted in sheep after in utero fetal intra-hepatic injection (IHI). In contrast, neither 13Lin nor 18Lin CD34 cells engrafted in either mice or sheep when transplanted by regular routes (i.e., TVI and fetal IHI, respectively), although both 13Lin and 18Lin CD34 cells engrafted in mice when transplanted by IBMI and exhibited multilineage reconstitution ability. Thus, the homing ability of CD34 HSCs is significantly more limited than that of CD34 HSCs. As for 18Lin, CD34 HSCs are characterized by low expression of the tetraspanin CD9, which promotes homing, and high expression of the peptidase CD26, which inhibits homing. This unique expression pattern homing-related molecules on CD34 HSCs could thus explain in part their reduced ability to home to the BM niche.

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Proliferative and Migratory Potentials of Human Cord Blood-Derived CD34 - Severe Combined Immunodeficiency Repopulating Cells That Retain Secondary Reconstituting Capacity

Cited by 18 publications

References 28 publications

In vivo dynamics of human cord blood-derived CD34− SCID-repopulating cells using intra-bone marrow injection

In vivo dynamics of human cord blood-derived CD34− SCID-repopulating cells using intra-bone marrow injection

Human CD34-negative Hematopoietic Stem Cells

CD34-negative hematopoietic stem cells show distinct expression profiles of homing molecules that limit engraftment in mice and sheep

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