Proliferative and morphologic characterization of buccal mucosal fibroblasts in areca nut chewers: A cell culture study

Mathew, Deepu George; Skariah, K Skariah; Ranganathan, Kannan

doi:10.4103/0970-9290.94693

Cited by 10 publications

(12 citation statements)

References 5 publications

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“…Mathew et al reported mitotic (FI, FII and FIII) population of fibroblasts cultured from tissues of patients with normal oral mucosa; they observed that FI population of cells have a spindled morphology which stained positive for vimentin. [12] Similar fibroblast morphology was also observed and reported by Bayreuther et al in skin fibroblasts. [13] Studies done by Waal et al reported that fibroblasts cultured from patients with normal mucosa and no areca nut chewing habit were of the FI (spindle) type when compared to the FIII type (large pleomorphic and epithelioid) in OSMF patients.…”

Section: Discussionsupporting

confidence: 84%

Characterization of oral fibroblasts: An in vitro model for oral fibrosis

Adtani

Narasimhan

Ranganathan

et al. 2019

J Oral Maxillofac Pathol

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Background: Oral submucous fibrosis (OSMF) is a chronic debilitating condition of the oral mucosa that has been classified as a potentially malignant disorder with a malignant transformation rate of 2%–8%. Several in vitro and in vivo experiments have been performed to formulate a treatment modality for OSMF, yet no ideal in vitro primary oral fibroblast model has been developed. Aim: To establish an in vitro primary oral fibroblast model. Setting and Design: In vitro laboratory setting. Materials and Methodology: Primary cell culture protocol was performed after obtaining normal oral tissue. Karyotyping was performed to rule out chromosomal abnormalities. Immunofluorescence staining was carried with a panel of fibroblast-specific markers (vimentin, phalloidin, transforming growth factor-β receptor 1 [TGFβR1] and s100a4) and Masson trichrome staining (MTS) to demonstrate the presence of extracellular matrix (ECM) qualitatively. Results: A monolayer of oral fibroblasts was observed on the 9 th -day postseeding. No chromosomal abnormality was observed in the patient samples. Positive staining was observed with vimentin, phalloidin, TGFβR1 and s100a4, thereby confirming the cell type. MTS revealed fibroblasts with spindle morphology and scanty ECM. Conclusion: The present study lays down a protocol to design and characterize primary buccal fibroblast cell culture model that would aid researchers in performing in vitro preliminary experiments in areas concerning fibrosis.

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Section: Discussionsupporting

confidence: 84%

Characterization of oral fibroblasts: An in vitro model for oral fibrosis

Adtani

Narasimhan

Ranganathan

et al. 2019

J Oral Maxillofac Pathol

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“…It is well accepted that excessive proliferation of fibroblasts is one of the major features of OSF [ 15 , 16 ]. To explore the role of CypA in the regulating fibroblast cell viability, human primary oral fibroblast cell and human fibroblast cell line IMR90 used as in vitro models.…”

Section: Resultsmentioning

confidence: 99%

Proteomic identification of cyclophilin A as a potential biomarker and therapeutic target in oral submucous fibrosis

et al. 2016

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Oral submucous fibrosis (OSF) is a pre-cancerous lesion, which is characterized by fibrosis of the oral submucosa. Despite large body of studies focusing on this disease, the molecular mechanisms underlying the progression of OSF remained unclear. In this study, 2-DE-based proteomic approaches were employed to identify the differently expressed proteins between OSF and normal tissues. In total, 88 proteins were identified with altered expression levels, including CypA. Upregulation of CypA was further validated through immunohistochemistry staining combined with Q-PCR and western blot by using clinical samples. Statistical analyses reveal that CypA expression level is correlated to the progression of OSF. Finally, functional study reveals a pro-proliferative property of CypA in fibroblast cells by using multiple in vitro models. The present data suggest that CypA might be a potential biomarker and therapeutic target for OSF, and will lead to a better understanding of OSF pathogenesis.

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“…1), they contain a subpopulation of fibroblasts-like small spindleshaped cells of non-uniform nature in SMF-F cells too. The [18]. This pleomorphic nature of culture may be due to the lack of natural defence and inherently irreversible DNA double-strand breaks [17,19].…”

Section: Discussionmentioning

confidence: 99%

Buccal Mucosal Epithelial Cells Downregulate CTGF Expression in Buccal Submucosal Fibrosis Fibroblasts

Gottipamula¹,

Sundarrajan²,

Moorthy³

et al. 2017

J. Maxillofac. Oral Surg.

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Proliferative and morphologic characterization of buccal mucosal fibroblasts in areca nut chewers: A cell culture study

Abstract: There was a significant increase in the pro-fibrotic, post-mitotic subpopulation of fibroblasts in areca nut chewers with OSF.

Cited by 10 publications

References 5 publications

Characterization of oral fibroblasts: An in vitro model for oral fibrosis

Characterization of oral fibroblasts: An in vitro model for oral fibrosis

Proteomic identification of cyclophilin A as a potential biomarker and therapeutic target in oral submucous fibrosis

Buccal Mucosal Epithelial Cells Downregulate CTGF Expression in Buccal Submucosal Fibrosis Fibroblasts

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