Proliferative and Non-Proliferative Lesions of the Rat and Mouse Soft Tissue, Skeletal Muscle and Mesothelium

Abstract

The INHAND Project (International Harmonization of Nomenclature and Diagnostic Criteria for Lesions in Rats and Mice) is a joint initiative of the Societies of Toxicologic Pathology from Europe (ESTP), Great Britain (BSTP), Japan (JSTP), and North America (STP) to develop an internationally accepted nomenclature for proliferative and nonproliferative lesions in laboratory animals. The purpose of this publication is to provide a standardized nomenclature for classifying lesions observed in the soft tissues including skeletal muscle as well as the mesothelium of rats and mice. The standardized nomenclature of lesions presented in this document is also available electronically on the Internet (http://www.goreni.org/). Sources of material included histopathology databases from government, academia, and industrial laboratories throughout the world. Content includes spontaneous developmental and aging lesions as well as those induced by exposure to test materials. A widely accepted and utilized international harmonization of nomenclature for lesions in soft tissues, skeletal muscle and mesothelium in laboratory animals will decrease confusion among regulatory and scientific research organizations in different countries and provide a common language to increase and enrich international exchanges of information among toxicologists and pathologists. (DOI: 10.1293/tox.26.1S; J Toxicol Pathol 2013; 26: 1S–26S)

Residual sodium dodecyl sulfate in decellularized muscle matrices leads to fibroblast activation in vitro and foreign body response in vivo

“…Foreign body giant cells (FBGC), which appear in H&E stained tissue sections as overlapping nuclei with a shared cytoplasm, were visible surrounding the DM‐SDS hi implants (Figure b, left panel). Hemosiderin deposition was apparent in all DM‐SDS hi treated samples (Figure b, right panel), which is associated with the foreign body response and soft tissue necrosis (Greaves et al, ; Higgins et al, ). The number of hemosiderin‐laden cells were counted in 40× fields of view, with the results shown in Figure c.…”

“…In Figure 5a (Figure 5b, left panel). Hemosiderin deposition was apparent in all DM-SDS hi treated samples (Figure 5b, right panel), which is associated with the foreign body response and soft tissue necrosis (Greaves et al, 2013;Higgins et al, 2009). The number of hemosiderin-laden cells were counted in 40× fields of view, with the results shown in Figure 5c.…”

Lung, Pleura, and Mediastinum

“…Primary spontaneous pleural mesotheliomas are rare in rats. They have been induced by exposure to various fibers and chemicals (Greaves et al, 2013;Kane, 2006;Doi et al, 2010;Wagner, 1962;Stanton and Wrench, 1972). Grossly, pleural mesotheliomas may be focal or diffuse along the pleura, and grow by expansion rather than infiltration.…”

Histopathological Evaluation of Skeletal Muscle with Specific Reference to Mouse Models of Muscular Dystrophy

“…Consultation with a veterinary pathologist or researcher experienced in evaluating the mouse model in question is advised before the start of the experiment if the user is unfamiliar with the model. For more detail on spontaneous laboratory animal muscle pathology, the reader is referred to the International Harmonization of Nomenclature and Diagnostic Criteria (INHAND) publication on soft tissue and skeletal muscle (Greaves et al, 2013).…”