Proliferative Effect of Tilapia Fish (Oreochromis niloticus) Lectin on BALB/c Mice Splenocytes

Abstract

Background: Lectins have been studied in recent years due to their immunomodulatory activities. Objective: We purified a lectin named OniL from tilapia fish (Oreochromis niloticus) and here we analyzed the cell proliferation and cytokine production in Balb/c mice splenocytes. Methods: Cells were stimulated in vitro in 24, 48, 72 hours and 6 days with different concentrations of OniL and Con A. Evaluation of cell proliferation was performed through [3H]-thymidine incorporation, cytokines were investigated using ELISA assay and cell viability assay was performed by investigation of damage through signals of apoptosis and necrosis. Results: OniL did not promote significant cell death, induced high mitogenic activity in relation to control and Con A and stimulated the cells to release high IL-2 and IL-6 cytokines. Conclusion: These findings suggest that, like Con A, OniL lectin can be used as a mitogenic agent in immunostimulatory assays.

The Deposition of a Lectin from Oreochromis niloticus on the Surface of Titanium Dioxide Nanotubes Improved the Cell Adhesion, Proliferation, and Osteogenic Activity of Osteoblast-like Cells

“…Previous studies showed that mannose-binding lectins as Lens culinaris (lentil) lectin (LcL) and Narcissus pseudonarcissus (daffodil) lectin (NpL), a α-d-mannose-binding protein, did not elicit potent cytotoxicity against osteosarcoma cells [ 56 ]. Furthermore, a study by da Silva et al [ 57 ] showed that OniL was able to favor the proliferation of Balb/c splenocytes without causing significant cytotoxicity to these cells. Furthermore, our fluorescence microscopy assay showed that the cells remained adhered to the surface of TiO 2 NTs, presenting a preserved morphology and nuclei integrity throughout the experiments.…”